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The invention belongs to the fluid measuring technical field and relates to an oil, gas and water three-phase oil well continuous gauging device, which comprises a high-efficient gas-liquid separator; the high-efficient gas-liquid separator is connected with an oil, gas and water three-phase inflowing port; a gas phase outlet of the oil, gas and water three-phase inflowing port is connected with a gas phase measuring pipeline; a gas path temperature sensor, a gas path pressure sensor and a gas path endocone flowmeter with high-frequency differential pressure transmitter are arranged on the gas phase measuring pipeline; a liquid phase outlet of the high-efficient gas-liquid separator is connected with a liquid phase measuring pipeline; a liquid path Coriolis mass flowmeter and a liquid path endocone flowmeter are arranged on the liquid phase measuring pipeline; and the cross section of the pipeline for mounting the liquid path Coriolis mass flowmeter is more than the cross section of the pipeline for mounting the liquid path endocone flowmeter.

本发明属于流体测量技术领域,涉及一种油、气、水三相油井连续计量装置,包括高效气液分离器,高效气液分离器与油、气、水三相流入口相连,其气相出口与气相测量管路相连,在气相测量管路上装有气路温度传感器、气路压力传感器、带有高频差压变送器的气路内锥流量计;高效气液分离器的液相出口与液相测量管路相连,在液相测量管路上装有液路科氏质量流量计和液路内锥流量计,安装液路科氏质量流量计的管路的截面积大于安装液路内锥流量计的管路的截面积。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果:倒置显微镜和透射电镜观察显示,经SAHA处理的细胞增殖速度显著减慢,细胞体积增大,细胞核较小,形状较为规则,核仁数量减少、体积变小,核内常染色质增多而异染色质减少,核质比例减小,细胞质内线粒体数量增多、线粒体嵴发达,高尔基体较为典型,粗糙型内质网增多,呈现出与正常上皮细胞相似的形态变化;MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用,并有明显的剂量依赖和时间依赖关系;免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达;流式细胞仪检测结果显示,SMMC-7721细胞经SAHA处理后,G0/G1期细胞明显增加,S期细胞则明显减少,细胞被阻滞于G0/G1期;RT-PCR检测结果表明,SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加,24h后更为明显。

To predict the accident scenario caused by the total instantaneous inlet blockage of single fuel subassembly at full power in fast reactors, a model was developed according to the SCARABEE experiments. The calculation results for the SCARABEE BE+1 experiment agree well with the experimental observation. The model was then applied to an actual reactor to predict the consequences of TIB.

为了预测正常功率下快堆单个燃料组件入口完全堵流所导致的事故序列,根据SCARABEE系列堆内实验建立了模型,针对SCARABEE BE+1实验的计算结果与实测数据吻合,进一步使用该模型对实际快堆中的单组件完全堵流事故进行了预测。

Practically, there are unsteady flow in the water diversion system of the pumping station as a result of the change of upsteam stage and pump operation stage. It has important significance for the optimum operation and management of the pumping station to study on one-dimensional unsteady flow of water diversion system of the pumping station.

同时在泵站实际运行过程中,由于上游水源水位和泵站运行水位变化的影响,引水系统内往往会产生非恒定流,因此,泵站引水系统非恒定流计算研究对泵站的优化运行和管理具有重要的理论意义和实际应用价值。

Our experimental measurements reified this new theoretical model of describing the capillary filling behavior. The dynamic contact angle depends on Oh and f Re, the capillary filling distance scales with the square of capillary filling time.

依据描述该现象的理论关系式,发现方型微流道内表面张力流毛细填充现象与动态接触角α、欧氏数Oh与无因次化参数f Re有关,藉由实验量测的方式,发现动态接触角为欧氏数Oh及f Re值的函数。

They help us understand the regulation of inner gas-flow field of coal ball mill and lay a foundation for further study in gas-particle property of the machine. At the same time, the conclusions offer beneficial reference for optimizing the machine structure design and controlling the inner flow field.

文中总结出磨煤机内气相流场随磨煤机填充率和进出口管道口径变化的规律,为磨煤机的结构优化设计和实际生产中对磨煤机内部流场进行控制提供了有益的参考。

The asymmetry of gas flow field in a cyclone separator was studied based on the flow field predicted by Fluent 6.1 with ASM model.

采用Fluent6.1软件提供的代数应力模型计算了旋风分离器内的气相流场,并在此基础上讨论了流场的非轴对称特点。

The technique does not have any simplification of drainage holes. In this paper, discussions are mainly conducted on the substructure mesh dividing and its second time automatic dividing, the substructure shape, the continuity among substructures and general elements, the classification and definition of the integrated hydraulic model of drainage substructure, the iteration computation of water level inside drainage hole, the application of the technique in solving unsteady unsaturated seepage field, etc., therefore, the high efficient, precise and quantitative analysis of the complex seepage with densely distributed drainage holes is totally realized.

该技术不对排水孔幕作任何简化,本文着重从子结构网格划分模式及二次自动剖分、子结构单元形态、子结构与总体单元网格之间的连续性、排水子结构完整水力学模型分类及定义、排水孔内水位迭代计算、排水子结构法在非稳定非饱和渗流场中的推广应用等各个方面进行论述,实现了对工程复杂渗流场中密集排水孔幕的高效、精细、定量分析。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞,运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化;用gp120处理并用anti-gp120-FITC进行染色,运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况;用抗磷酸化ERK 抗体免疫荧光方法进行染色,运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Passenger flow models for walkway, upward stairway and downward stairway which illustrate passenger speed/density relationships have been built using statistical method based on the collected data at different types of passenger facilities in urban railway transit hubs.

然后针对北京市几个主要轨道交通枢纽进行了大量乘客交通流数据的观测和采集,建立枢纽内进站通道、出站通道、换乘通道、上行楼梯和下行楼梯处的速度-密度关系的交通流解析模型,得。。。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

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本文综合考虑了几种电弧重燃和熄灭理论。