内流的

Numerical Simulation of Flow Field in Pulsating Fluidized Bed Under special entry conditions, the CFD is used to simulate the three-dimension fluidized bed with different oscillation intensity and frequency in order to research the effect of sound oscillation on flow field in the fluidized bed. The results indicate that the sound oscillation can make the distribution of the flow field more uniform and can promote the heat and mass transfer.

脉动流化床流场模拟在特定的入口条件下，应用计算流体力学技术对三维流化床在不同振动强度、不同频率扰动作用下的流场进行模拟，得到声波扰动对流化床流场分布的影响，结果表明声波振动可以使流化床内的流场分布更加均匀，促进热质传递。

It was important that the thixotropic agent,surface active agent and solid lubricating agent are added,the surface of magnetizable particles were suitable treated,at the same time,the stability and lubricating property of MRF were analyzed and discussed.

通过触变剂与表面活性剂复合使用，同时对磁性悬浮相表面进行适当的处理，并加入适当的固体润滑剂合成了一种稳定性和润滑性能良好的磁流变体材料，该磁流变体材料性能为：0η≤1000mPa.s，τ0.5T≥60kPa，半个月内无明显沉降，无板结，"四球机法"测量其润滑性能为磨斑直径。75mm，该类型磁流变液用于磁流变阻尼器200万次振动试验取得了理想的试验效果。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results: The light microscope pictures of group A(no ［α-32P］ ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq ［α- 32P］ATP in the perfusate) showed that distribution of numerous ［α-32P］ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel.

结果：灌流液中不加［α-32P］ATP的A组光镜照片显示大鼠肝细胞内外均未见特异性标记银粒显影，而灌流液中含37 MBq［α-32P］ATP的B组光镜照片显示大鼠肝细胞内可见多个大小不等的［α-32P］ATP自显影银粒分布，而肝窦及肝小叶中央静脉等血管内则几乎无黑色自显影银粒分布。

The model is used to count several engineering example.Comparing the 2D numerical results with electrical simulations,it is proved that the seepage model can count the seepage surface and the location of search results in dam well and truly.Comparing 3D results with 2D results at close section,the results are tally well except the difference of meshes and the interceptive position of section.So the model is credible and has upper practicality value.In the projects,the spending of account is increasing because of the bigger account field.

将饱和—非饱和渗流模型用于几个工程实例，其二维模拟结果同渗流电模拟实验结果进行了对比，证明本文的渗流模型完全能够准确计算坝内的渗流浸润线以及逸出面位置；三维数值模拟的结果在相近剖面处与二维结果作了比较，考虑三维网格剖分及剖面截取位置的差异，三维结果与二维结果吻合的较好，证明本文的饱和—非饱和渗流模型在三维计算中是可靠的且具有较高的实用价值。

The effects of longshore currents ,Stokes mass transport by waves,large scale eddies and the instability of longshore currents on the transport of pollutant in a surf zone are discussed based on the experimental results.

通过物理模型实验对海岸波浪作用下污染物运动特性进行了分析，重点分析了质量输移流、沿岸流、沿岸流不稳定运动及破波带内旋涡运动等海岸水动力因素的影响。

The structure, flow field distribution and the dust separating procedures of inner speeding vortex cyclone collector are very different from that of traditional cyclone dust collectors.

混流区和中心旋流区是内旋流加速旋风集尘器的主要特征之一。

Numerical simulation of the steady cavitating turbulent flow in the whole flow passage of a Kaplan turbine has been carried out by using a full cavitation model and a mixture two-phase flow model.

采用一种完整空化模型和一种混合流体两相流模型，对轴流式水轮机全流道内的定常空化湍流流动进行了数值模拟。

Flow characteristics and blocking flow effect of refrigerant in electronic expansion valve during regulating process are analyzed.

深入分析液体在调节阀中的各种流动状态及其闪蒸和气蚀现象产生的条件后，针对电子膨胀阀内制冷剂流动状况，进行了制冷剂流态判定，揭示了节流过程中电子膨胀阀内制冷剂流动特性和阻塞流影响。

The gas-solid flow s,especially,the flow distribution and separation efficiency in VQS with different isolate cyl.

采用Fluent软件对VQS系统内气-固两相流进行了数值模拟，重点考察了A、B、C型隔流筒的流场分布和分离效率。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！