内流的

Experimental study on hydraulic characteristics of underflow for energy dissipator with step-down floor

现对跌坎式底流消能工消力池内水流流态的演变进行了初步分析，随着入池能量的变化，消力池中会产生混合流、淹没混合流、淹没底流以及远驱底流流态。

The first step of simulation method is to establish the one-dimension parabolic partial differential advection diffusion equation that was used to describe the mass transportation model of air-pollution distribution as a block program model of the controllable plant. Then, applying the Fuzzy logic theory to operate tunnel jet-fans and control the tunnel wind quantity and adjust the wind average velocity would not let the air-pollution concentration over the standard safety value too much.

模拟方式首先将常被用以表示隧道内空气污染物质传理论模式之一维向平流扩散抛物线型偏微分方程式建构成一组可控制对象的方块程式模拟模型，再运用模糊逻辑理论模拟控制喷流式通风机之运转风量来调节隧道内平均风速以控制隧道内空气污染物浓度不至於高过标准安全值太多。

In this thesis, the studies as follows:(1) The axis and radial flow field distribution of CFB were analyzed by the particle flow visualization software, and the criterion of turbulence to circulation fluidization situation was reported.

本文以管式布风条件下流化床内流动特征为研究对象，主要研究内容如下：(1)通过开发的颗粒流场可视化软件，对循环流化床内轴径向的流场进行了分析。在循环流化床试验台上分段测量了全场颗粒矢量速度，得到了循环床上升段内的整个流场分布。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

It simulation results proves that when the inner pipes in the concentric annulus pipes stand still, the turbulent flow nucleus area approaches inner pipes; and when inner pipes circumvolve, the turbulent flow nucleus area approaches outer pipes. The shearing velocity near the inner pipes is the biggest, and the shearing velocity increases with the velocity of the inner pipes increasing.

模拟结果表明：在同心环空管道中当内管静止时，紊流核心区即轴向速度最大值区域靠近内管壁；当内管旋转时，紊流核心区即轴向速度最大值区域靠近外管壁；靠近内管壁处的切向速度最大，且随着内管转速的增加切向速度增大。

The simulation results of the cylinder-block model were given in the article, consistent with that of Middlemen"s. It was shown that the block part could improve the uniformity of the flow distribution. The analysis of the flow field of some materials with different power-law factor in the die designed by Mckelvey was close to that of Mckelvey"s. It was shown that the flow distribution was uniform in the die that can producesheets with 1200mm in width. During the analysis the flow channel was divided into several parts to get the details about the fluid flow in each area. A conclusion was drawn after analyzing the influence of the die pressure on the flow distribution, that the higher pressure is good for the output and bad for the uniformity of the distribution. Simulation of the die applying the cylindrical main runner showed the fact that when the cylinder replaced the cone the change of uniformity was trivial. The uniformity of the non-block flow channel was so poor that the block in the proper position of the die channel was necessary. The project of the die for the wider sheet was put forth, and the analysis of the fluid flow in the die was shown.

本文模拟了圆柱管缝隙流模型的三维流动场，得到了与Middlemen结论一致的流动均匀性，并通过模拟直观地展示了采用阻尼块可以改善挤出均匀性的现象；分析了多种幂率指数的流体在Mckelvey等人设计的流道模型中的流动分布，得到了与Mckelvey的分析结果接近的压力降和流量偏差；本文建立了1200mm幅宽机头流道的三维有限元模型，并逐个分析各个区域和多个截面的流动分布状况，由此揭示了1200mm幅宽L-型机头流道内的流北京化工大学硕士学位论文动分布基本均匀，并在分析了机头压力对于挤出流动分布的影响后得出结论：较高的机头压力可以获得较大的产量，但是不利于物料均匀分布；模拟了采用圆柱管主流道时机头流道内的流动分布，得出结论：主流道由圆锥管改为圆柱管时对流动均匀性影响不大，通过设置适当的阻尼仍然可以达到挤出平衡；模拟了两端进料式圆柱管无阻尼流道的流动均匀性后得到结论：两端进料式无阻尼结构的流动均匀性较差，宽机头中仍然有必要设置阻尼块；确定宽幅机头流道的基本方案，并设计了一种宽幅防水卷材挤出机头流道的结构，通过流动模拟表明该种结构的流动均匀性较好；考察了宽幅机头对于不同厚度片材的挤出适应性，得到结论：当生产不同厚度的片材时，阻尼块高度需要调整。

Through the analysis of intake valve exit three dimensional flow field and its angular momentum flux of four valve diesel engine under different plans, the law of in cylinder air angular momentum flux by the influence of disturbance between two intake ports was discovered.

利用测得的四气门柴油机气门口三维流场，分析了进气门出口流场产生的缸内空气动量矩流率，揭示了四气门柴油机两进气道的相互干扰对缸内空气动量矩流率影响的变化规律。

Accoding to the gained velocity and pressure fabric, fluids' flow mechanism in the flow channel was researched, and the effects of changes of many parameters such as geometical, material and processing parameters on the flow field's pressure and velocity fabric in the flow channel of pipe die for extrusion were analyzed and discussed.

代入管材常用挤出塑料的参数，利用ANSYS有限元软件进行了三维流场计算，对物料在流道内的流动过程进行了模拟，求出了流场的速度分布和压力分布，研究了物料在机头流道内的流动机理。

Ultimately, we calculate steam function field, velocity field, pressure field and stress field.

最终，得到了粘弹性流体在扩张流道内流动时的速度场、流函数场、压力场和应力场以及在带横向槽流道内流动时的速度场和流函数场。

Instable flowing of plastics in extrusion die head often occurs as the sprue in the die head for plastic profiled bar inner rib is designed excessively dependable on experiences.

针对塑料异型材挤出模头内筋流道设计中过多依赖经验，造成模头作业不稳定的状况，提出了稳定性设计问题，并以实例介绍了运用计算机数值模拟技术辅助设计内筋流道结构参数的过程，提供了一个可行的内筋流道稳定性设计方法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！