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Morphological and flourescent changes were assessed using confocal microscopy in whole-mount organ of Corti preparations. Results (1) After the animals were exposed to broadband noise at 122 dB SPL in 4 h/day for 2 days, both apoptosis and necrosis appeared in OHCs. The single strand DNA in apoptotic OHCs were observed both in guinea pigs and mice.(2) In normal OHCs, EndoG was distributed outside of nuclei. EndoG translocated from outside to inside of the nuclei in both apoptotic and necrotic OHCs following noise exposure.(3) The MNNG cochlear perfusion and noise exposure both caused the transloctation of AIF from the mitochondria to the nuclei. The translocation of AIF took place in both apoptotic and necrotic OHCs.

结果 (1)暴露于120 dB SPL的白噪声环境中每天4小时,连续2天后引起豚鼠和小鼠耳蜗外毛细胞凋亡时,其细胞核内产生ssDNA,而在正常细胞内没有三ssDNA;(2)在正常情况下,EndoG分布于耳蜗毛细胞的细胞核外,在暴露于上述噪声后发生凋亡和坏死的豚鼠耳蜗外毛细胞中,EndoG从细胞核外转移到细胞核内,细胞核中的EndoG显著增加;(3)豚鼠耳蜗外淋巴灌流烷化剂MNNG后发生耳蜗外毛细胞凋亡和坏死,在凋亡和坏死的耳蜗外毛细胞中,AIF自线粒体转移到细胞核,其变化与噪声损伤引起耳蜗外毛细胞凋亡和坏死时一致。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系,应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后,流式细胞仪技术检测CCR1和CCR2的表达,ELISA法检测细胞培养上清液内白介素-8的浓度;体内实验选用C57BL/6J小鼠,腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg),LPS刺激4 h后收集BALF和肺脏标本,计数BALF内细胞总数,应用BCA法检测BALF内蛋白浓度,Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h,MTT法检测ox-LDL对RAW264.7细胞生长的影响;流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡;用特异性siRNA沉默Daxx在RAW264.7细胞中的表达,通过AO/EB染色观察基因沉默后的细胞凋亡形态学改变;高效液相色谱检测细胞内胆固醇含量;油红O染色观察细胞内脂滴的形成情况;Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况;用Western Blot印迹法检测caveolin-1蛋白的表达。

Three kinds of swirl meter are used to measure the swirl in different cross section of a diesel engine cylinder on a stationary flow test bench. This paper analyses the influence on incylinder swirl measurement of the type of swirl meter, measuring position and flow distribution in-cylinder.

本文在稳流模拟试验台上,利用三种涡流计测量了不同横截面高度的缸内涡流,分析了涡流计型式及测速位置对缸内涡流转速测量结果的影响,阐述了缸内速度分布对涡流测量影响的变化规律。

In the recent 10 years, at the three special fields of dynamic aerodynamics, although the circumferential Reynolds number gap between wind tunnel free flight and its corresponding real flight is as high as one or two magnitudes, wind tunnel free flight experiment results succeed in predicting or reproducing the dynamic aerodynamic characteristics in the three special fields, which appear in the real flight phase producing severe effects on flight security and flight performance.

近10余年来,在3个动态气动专题领域内,地面风洞自由飞实验与对应的天空飞行器的绕流雷诺数虽然相差1~2个量级,但风洞自由飞实验结果多次预测或再现了天空飞行器出现的对飞行安全、飞行性能产生严重影响、其它地面风洞实验方法难以预测或再现的这3个专题领域内的动态气动特性,这至少为3个专题领域内天地动态气动相关中的雷诺数影响,提供了一个新的理解。

Results All patients underwent percutaneous cholecystostomy with technical success without major periprocedural complications. 93 (96%) patients responded well clinically showing disappearance of right upper abdomen pain and fevers within 72hr. 4 patients complicated with common bile duct stones were underwent PTCD one day after the procedure because of no response clinically. After PTCD, all 4 patients attained clinical symptom relief within 72hr.

结果 所有97例患者均一次置管成功,无任何并发症。93例(96%)患者临床体征如右上腹疼痛、发热在72h内缓解;同时,血常规中的WBC及中性粒细胞均于72h内降至正常范围。4例患者由于肝总管内结石合并胆管炎临床症状缓解不明显而于第2天行经皮肝穿胆管引流术,术后临床症状于72h内亦缓解。

The invention is characterised in that the gaseous fuel containing hydrogen or consisting of hydrogen is fed into the swirl generator (1) largely axially and/or coaxially to the burner axis, forming a fuel flow, with a largely spatially defined flow form (9) which is maintained inside the burner and opens up in the region of the burner outlet.

本发明的特征在于,所述含有氢气或者由氢气所组成的气态燃料在涡流发生器(1)内相对于燃烧器轴线尽可能轴向地和/或同轴地、在形成燃料流的情况下以尽可能空间受限制的流动形状(9)被输入,该流动形状(9)在燃烧器内得以保持,并且在燃烧器出口的范围内绽开。

And some knowledge of grid topology is also introduced. The nucleation of condensative steam in the whole flowing field has been displayed. Furthermore, the characteristic of two-phase complex flow is analyzed. In the stationary blade's passage, the flowing status is stable relatively. The steam expanding is comparatively relaxing and the nucleation area of steam is small. In the moving blade's passage, the expanding of steam is tempestuous relatively because of dual-velocity effect; the degree of condensation of steam is considerably distinct.

最后,对有凝结的蒸汽在三维叶栅中的流动进行了模拟,得到了蒸汽凝结流动在整个流道内的流动情况,分析了这种两相复杂流动的特点,在静叶内,流动状态基本稳定,蒸汽膨胀较为缓和,发生凝结的区域很小;在动叶内,由于双重速度的作用使得蒸汽膨胀较为剧烈,相对蒸汽凝结程度比较大。

It is also found that the electroosmotic flow is in the opposite direction to that predicted by the classical continuum theory.

模拟结果表明,在双电层的某个区域内,同号离子的浓度超过了对离子的浓度,出现了电荷倒置现象;约占整个通道的三分之二内的区域内,溶液朝着与连续性理论预测的相反方向流动,出现了反转电渗流。

The invention belongs to the fluid measuring technical field and relates to an oil, gas and water three-phase oil well continuous gauging device, which comprises a high-efficient gas-liquid separator; the high-efficient gas-liquid separator is connected with an oil, gas and water three-phase inflowing port; a gas phase outlet of the oil, gas and water three-phase inflowing port is connected with a gas phase measuring pipeline; a gas path temperature sensor, a gas path pressure sensor and a gas path endocone flowmeter with high-frequency differential pressure transmitter are arranged on the gas phase measuring pipeline; a liquid phase outlet of the high-efficient gas-liquid separator is connected with a liquid phase measuring pipeline; a liquid path Coriolis mass flowmeter and a liquid path endocone flowmeter are arranged on the liquid phase measuring pipeline; and the cross section of the pipeline for mounting the liquid path Coriolis mass flowmeter is more than the cross section of the pipeline for mounting the liquid path endocone flowmeter.

本发明属于流体测量技术领域,涉及一种油、气、水三相油井连续计量装置,包括高效气液分离器,高效气液分离器与油、气、水三相流入口相连,其气相出口与气相测量管路相连,在气相测量管路上装有气路温度传感器、气路压力传感器、带有高频差压变送器的气路内锥流量计;高效气液分离器的液相出口与液相测量管路相连,在液相测量管路上装有液路科氏质量流量计和液路内锥流量计,安装液路科氏质量流量计的管路的截面积大于安装液路内锥流量计的管路的截面积。

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