内流

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Next the discussion in this study, about an ellipse with an angle of attack and two circular cylinders with different diameters in the flow, the aim of this study is investigated for the ability of the present numerical method by solving the suppression of the vortex shedding flow behind the two-cylinder system which was kind of difficult to reach by a Cartesian grid method. In this study, the suppression of the vortex shedding flow is tested to solve a uniform flow past through two different diameter cylindrical in the flow field with Fractional Step Method and Immersed Boundary Method.The numerical method which we investigated is showed that the results of the problem is appropriable in other literatures.

接著探讨流过具攻角之椭圆柱体流场及流过圆柱抑制涡漩逸出之流场，目的是要研究利用控制圆柱来抑制下游涡流的流场行为，本文采用分步法配合内嵌边界法数值解析一均匀流流经两不同直径的圆柱流场，来测试抑制涡流逸出的问题，根据现有的数值结果发现试验运用了合适的方法且得到令人满意的结果。

The model is used to count several engineering example.Comparing the 2D numerical results with electrical simulations,it is proved that the seepage model can count the seepage surface and the location of search results in dam well and truly.Comparing 3D results with 2D results at close section,the results are tally well except the difference of meshes and the interceptive position of section.So the model is credible and has upper practicality value.In the projects,the spending of account is increasing because of the bigger account field.

将饱和—非饱和渗流模型用于几个工程实例，其二维模拟结果同渗流电模拟实验结果进行了对比，证明本文的渗流模型完全能够准确计算坝内的渗流浸润线以及逸出面位置；三维数值模拟的结果在相近剖面处与二维结果作了比较，考虑三维网格剖分及剖面截取位置的差异，三维结果与二维结果吻合的较好，证明本文的饱和—非饱和渗流模型在三维计算中是可靠的且具有较高的实用价值。

Performance prediction of the pump when transporting water and salt solution is also studied.Results are compared and analyzed with experimental data.6.The salt-out flow field is measured on three dimensions by Particle Image Velocimemter via radial and axial two test models.After image processing, velocity distribution of crystal particles in the pump is obtained.Particle flow field is visualized to reveal the particle motion law intuitionally.

此外对泵送清水和盐溶液时的性能也进行了预测，并将结果与外特性试验数据对比。6、运用PIV在径向和轴向两种测量模式下对泵内不同外部条件下盐析两相流场进行了准三维测量，经图像后处理，获得了盐析晶体颗粒在叶轮内、无叶腔中及轴面上的速度矢量分布，实现了颗粒流场的可视化，更为直观地揭示了泵内盐析颗粒的运动规律。

METHODS: 24 rabbits were divided into four groups: control group; rapid pacing group; adenosine perfusing group; selective adenosine receptor antagonist perfusing group. The index of observed: time of repolarization in monophase action potential (APD90); the active level of A1 adenosine receptor; the level of free calcium in the atrium cells and the ability of atrium cell to release calcium.

新西兰白兔24只，随机分为4组：①对照组：常规克氏液灌流，②腺苷灌流组：灌流液中加入2umol/L的腺苷，③心房快速起搏组：常规克氏液灌流+心房快速起搏，④腺苷受体拮抗剂干预组：克氏液+DPCPX（100nmol/L）灌流+心房快速起搏；观察指标：①起搏前后心房肌单相动作电位复极时间的变化；②起搏组和对照组的心房肌腺苷A〓受体活性对比；③心房肌细胞内游离钙含量以及氯化钾激动后钙释放能力对比。

The method comprises A: separately configuring different common channel priority indication for different service data stream transmitted on same dedicated service channel; B: according to sequence from high to low of public channel priority indication for different data stream, priorly scheduling public channel high priority indication service data stream for transmitting at every moment; C: if service data stream of low common channel priority indication being not scheduled in a preset reference time period, then upgrading the service data stream common channel priority indication, then return to B.

分别为在同一专用业务信道上传输的不同业务数据流配置不同的公共信道优先级指示；B。在每一时刻，所述专用业务信道按照不同业务数据流的公共信道优先级指示由高至低的顺序，优先调度公共信道优先级指示高的业务数据流进行传输；并C。预设一参考时间段，若公共信道优先级指示低的业务数据流在所述参考时间段内总没有得到调度，则将该业务数据流的公共信道优先级指示升高，然后返回B。

In order to study the intake air flow characteristics in four-valve diesel engines, in this dissertation hot-wire anemometer and PIV twodimension instantaneous measuring technique are used. Extensive experimental study on two intake valve exit flow fields and longitudinal section flow field in cylinder are performed for different type of diesel engines. Great researching progress have been made on the interaction between two intake ports, and the formation process of in-cylinder swirl and flow field structure of cross and longitudinal section for four valve diesel engines. These results are presented in China for the first time and without such kind of research work reported abroad as detailed and deepgoing as this dissertation. The author designs and makes a set of hot wire measuring velocity device to measure the velocity distribution around the intake valve annulus and the cross section flow field in cylinder.

为了研究四气门柴油机进气过程中气体流动的变化规律，本文利用热线测速和PIV二维瞬态测速技术，对四气门柴油机两进气门出口全圆周流速场和缸内横、纵剖面的气体流动流场进行了大量的试验研究，在四气门柴油机两进气道之间相互影响的变化规律，缸内涡流的形成过程及缸内横、纵剖面流场结构等方面取得了重大的研究进展，这一研究工作在国内尚属首次，在国外也未见有如此全面、深入的研究报道。

PDA(particle dynamics analyzer system) was used to measure the flow velocity of particles in the XFD-12 flotation cell, the results show that tangential mean velocity in the radial direction changes from positive to negative in the flotation cell, naught point emerge in the medium of the radial direction ,it is where backward fluid emerge ,shear stress of backward fluid induce the particle detachment from bubbles .

汁算数据与实际矿石筛析结果都表明，紊流强度增大，泡沫中粗粒级含量减小。 4。用PDA在XFD-12型8L浮选机中测定的速度值，进行混合区、分离区、泡沫区紊流运动功率谱计算，得紊流脉动频率，混合区小于20Hz，分离区在20-30Hz之间，泡沫区在25-35Hz之间，浮选槽内紊流脉动以低频为主，且紊流脉动动能是混合区大于分离区大于泡沫区。

Firstly in the paper the research of hydraulic characters and the optimize of structure have being carried out on the four bigger design schemes of permanent internal dissipation discharge tunnel reconstructed from the right banks diversion tunnel of Gongboxia Power Station with a method of theoretical analysis combined with test comparisons. On this basis , two form swirling flow internal dissipation schemes, vertical and horizontal axis are recommended to the construction unit of the station as the schemes to be selected next.

本文采取理论分析与试验比较相结合的方法，首先对黄河公伯峡水电站右岸导流洞改建为永久式内消能泄洪洞的四个较大设计方案进行了水力特性的试验研究与体型优化，在此基础上，向建设单位分别提供了竖井旋流内消能的推荐方案与水平旋流内消能的推荐方案。

Phase-lagged cyclic boundary condition is implemented by a novel method in conjunction with the developed sliding interface technique to accurately simulate the arbitrary pitch ratio rotor/stator interaction without any modifications of the physics of the problem. It's for the first time to compose the computational area into one structured grids including both rotor/stator of centrifugal or axial turbomachinery with arbitrary pitch ratio. This method can eliminate the iteration of boundary conditions on the sliding interface and calculate the unsteady flow within rotor and stator simultaneously.

为了能够在较小的计算区域内不改变叶型而真实地反映出任意栅距比时的内部非定常流场，并避免近似仍采用相位后滞周期性条件，结合本文发展的网格技术，给出了一种新的实施方法，首次将动、静叶片数不等的离心式/轴流式叶轮机械转子、静子计算区域组合在一套结构化计算网格中，动/静叶排间的滑移界面完全按内场处理，避免了每一时层内，滑移界面处边界条件的迭代计算；并保证了每一时层，滑移界面处流动参数的守恒特性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新