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The effect of tat p53 on the growth of parasites was determined by counting the parasitemia changes between pre and post incubation of the fusion protein with the parasite culture for 12 h. results: tat p53 fusion protein could be transduced into p.f parasites, locating at trophozoit and late schizont stage, whereas p53 protein could not.

结果:tat p53融合蛋白可以突破红内期疟原虫各层细胞膜,进入虫体细胞内,并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体,而p53蛋白不能进入疟原虫内。

The effect of TATp53 on the growth of parasites was determined by counting the parasitemia changes between pre and postincubation of the fusion protein with the parasite culture for 12 h. RESULTS: TATp53 fusion protein could be transduced into P.f parasites, locating at trophozoit and lateschizont stage, whereas p53 protein could not.

结果:TATp53融合蛋白可以突破红内期疟原虫各层细胞膜,进入虫体细胞内,并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体,而p53蛋白不能进入疟原虫内。

The anastomosing occurred between the pleural superficial and the subpleural vascular networks on the capillary level or the precapillary level. The honeycomb vascular networks were also various in size and form at a low magnification. The venule connected with the pulmonary capillary networks directly at a high magnification. The single capillary networks were found in the alveolar septa. The longitudinal crests and dense round imprints of endothelial nuclei and the clear circular constrictions were found on the surface of the larger vessel in the lung parenchyma. The round imprints of endothelial nuclei also occurred on the subpleural venule and capillary.

胸膜面浅层的微血管与胸膜下微血管之间形成毛细血管水平或毛细血管前水平的吻合;低倍镜下肺实质内微血管网也呈现大小不等、形态各异的蜂窝状结构;高倍镜下肺泡毛细血管与微静脉之间联系紧密;在肺泡隔内毛细血管网成单层分布;肺实质内管径较大的微血管表面可以见到纵行的嵴状结构和密集的内皮细胞核的压痕,以及明显的环形缩窄;胸膜下微静脉和毛细血管表面可见圆形内皮细胞核的压痕。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达,由原始生殖细胞产生卵原细胞,髓质退化成为卵巢腔,分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加,它们在分化结束后解体,分化前细胞内有较多的大颗粒,在分化中大颗粒减少,小颗粒增多,在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜,髓质有许多睾丸管构成,管腔内有散落排列的精原细胞。

Abstract] objective to improve the diagnosis of non-ossifying fiboma by analysing its x-ray feature.methods plain x-ray films was performed in all patients.x-ray finding in 8 cases with pathologically-confirmed non-ossifying were analyzed.results the clinical symptom was mild,the lesions occurred usually at the metaphysis of the long bones,the affected bones included femur(n=4),tibia(n=3),humerus(n=1).cortical type was seen in 5 cases,presenting as unilocular or multilacular transparent areas with in the cortex or tightly beneath the cortex,the lesion had a sclerotic margin.which was more obvious at the marrow side.medullary type was seen in 2 cases.the lesion was located at the center of the bone and grew centrally.the tumor was manifested as unilocular or multilocular.tramsparent area with sclerotic border and the bone cortex became thinner with slightly expanding on 11 sides.conclusion plain radiography is the elementally means to detect this disease.based on the typical x-ray signs of non-ossifying fibroma combined with clinical data,correct preoperative diagnosis can be made in most cases.

目的 探讨非骨化性纤维瘤的x线表现以提高诊断水平。方法所有病例均摄有x线平片,分析经病理证实的8例非骨化性纤维瘤。结果临床症状轻,好发于下肢长骨干骺端或骨干,股骨4例,胫骨3例,肱骨1例。皮质型5例,表现为皮质内或紧贴皮质下的单层或多层透亮区,病变向骨内发展进入髓腔,周围有致密硬化带环绕,以髓腔侧明显;髓质型2例,病灶在骨内中央发展,显示为单房或多房透亮区,边缘有硬化,骨皮质菲薄,轻微向周围膨隆。结论 x线为最基本的检查方法,典型病例x线平片结合临床症状即可明确诊断。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

It is also found that the electroosmotic flow is in the opposite direction to that predicted by the classical continuum theory.

模拟结果表明,在双电层的某个区域内,同号离子的浓度超过了对离子的浓度,出现了电荷倒置现象;约占整个通道的三分之二内的区域内,溶液朝着与连续性理论预测的相反方向流动,出现了反转电渗流。

Hemarthrosis and laceration of the disc and the attachment were also observed. 7 days later, the hemarthrosis was seldomly seen. Effusion was seen in some joints at 2 weeks. At 1 month posttrauma, the severely damaged structure of the two joints in one animal was found. We could find the loss of the disc and the cartilage of the condyle. Effusion also existed.

伤后1月,可见有一侧关节盘完全破坏,髁突软骨层消失,关节腔内渗出;对侧关节结构紊乱,关节盘及髁突明显变形、不规则等变化。3月后,多数关节结构基本正常,但关节内的破坏性改变仍然存在,关节内可见较明显的裂隙或囊状改变,软骨组织变薄,关节盘松解,关节腔扩张等变化。

Just obtain ad of Shanghai subway TV 5 years the DMG of the the sole agency to " daily of the first finance and economics " express, had been in the first ten days of a month received an announcement in Feburary, DMG respect points out all inside Shanghai subway railroad car screen does not have frequency signal originally, Shanghai subway from cannot phonate inside requirement railroad car at the beginning, and we belong to information of platform layer operation to show establishment in the screen inside the platform, volume is under 70 decibel, and the advertisement that our bus stands is in formulary limits less than.

刚刚获得上海地铁电视广告5年独家代理权的DMG向《第一财经日报》表示,已经在2月上旬接到通知,DMG方面指出在上海地铁车厢内所有屏幕原本没有音频信号,&上海地铁从一开始要求车厢内不能发声,而我们在站台内的屏幕属于站台层运营信息显示设施,音量都在70分贝以下,而我们公交车站的广告都在规定范围以内。&

Endoplasmic reticula in enlarged C-tapetal cells were aggregated in stacks at first, and then produced numerous ER-vesicles. As inclusions in the vesicles increased, the cytoplasm in these cells decreased until it was replaced by starch grains and lipid droplets.

膨胀的C-绒毡层细胞中,内质网先发生聚集,之后断裂并产生大量内质网小泡;随着内质网小泡内容物增浓,细胞质逐渐变稀;最后,细胞内容物几乎全部变成多糖颗粒和以脂类物质为主的滴状物。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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