内层

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Dat document, the free curve which connected by the multiple valid points was formed.

dat文件，形成由多个数据点连接的自由曲线，依次读取第2 层、第3 层、第4 层至第87 层，形成兔股骨内、外轮廓的堆叠图。

After reviewing lots of previous work on cell layout generation and standard-cell and BBL placement and routing technique, algorithms for transistor placement and intra-cell routing in cell layout generation, timing-driven standard-cell placement, multi-layer detailed area routing and multi-layer constrained via minimization are presented and implemented.

论文在较为深入研究和总结国内外有关单元版图自动生成及集成电路布局布线技术方面研究成果的基础上，对标准单元版图自动生成技术中单元内晶体管布局和单元内布线、时延驱动的标准单元布局、多层金属工艺的区域详细布线及多层布线通孔最少化进行算法研究、设计和实现。

In order to deeply analyze the distribution of maximum tensile strain during asphalt surface for Semi-rigid base asphalt pavement and compound base asphalt pavement , two types of typical structure were chosen, taking the Shell designing software BISAR 3.0 as calculation tool, considering two kinds interface contact condition of complete continuousness and not incomplete continuousness, then a comprehensive analysis was made on the maximum tensile strain of every structure on the basis different interface contact condition.

摘 要：为深入分析半刚性基层沥青路面和复合式基层沥青路面面层内的最大拉应变的规律，选取两类沥青路面的典型结构，采用壳牌设计软件 BISAR 3.0为计算工具，考虑层间完全连续状态以及不完全连续两种状态，对各结构在不同的层间结合条件下沥青层最大拉应变进行了比较和分析。

In the single crystal of C60 with a FCC structure,positron mainly appears outside the C60 molecule.The main annihilation space is the interspace between molecules.The calculated positron bulk lifetime in C60 is 352ps, which agrees with experiment value of 356ps in literature.In carbon nanotube bundles with different dimeters,as the diameter of carbon nanotubes increases,the main space where positron appears changes from the interspace of carbon tubes to the space inside carbon tubes,the radio between positron annihilation with valence eletrons and core eletrons becomes larger,the positron bulk lifetime in carbon nanotube increase rapidly first and come to be a constant at the end.The calculated positron lifetime of carbon nanotube with a dimeter of 0.8～1.6ns is 332～470ps,which agrees with the experiment value of 394ps.Positron annihilation has been studied in widly used compound semiconductors.

计算结果表明：在片层结构的石墨晶体中，正电子主要在石墨层间的空隙中湮没，计算出的石墨中的正电子寿命为208 pS，与文献中的实验结果215 ps符合很好；在金刚石单晶中，正电子主要在碳原子之间的空隙中存在并发生湮没，计算出的金刚石中的正电子寿命为115 ps，文献中的实验结果110 ps左右符合；在面心立方结构的C60晶体中，正电子主要在C60分子球壳内外侧及分子之间存在，C60球形分子中心正电子分布很少，正电子的湮没区域集中在C60分子之间的空隙区域，计算出的C60中的正电子寿命为352 ps与文献中的实验结果356ps相符合；对于不同管径碳米管束中的正电子分布，随着碳纳米管直径的增加，碳纳米管束中的正电子由主要在碳纳米管管间的区域出现转变为主要在碳纳米管管内中心的区域出现：碳纳米管束中的正电子与碳原子的价电子的湮没概率变得越来越大，与核心电子的湮没概率变得越来越小；碳纳米管束中正电子的湮没寿命先迅速增大，而后趋于一定值。

During this stage, the cells group into three layers, the first is the 'ectoderm' which then in turn generates the 'mesoderm' and 'endoderm' layers.

在这一阶段，细胞分化为3层，第一层是"外胚层"，它最终会形成中胚层和内胚层。

In addition, certain endoderm marker genes expressed in the early stage during in vitro pancreatic differentiation means that these cells are endoderm-like cells.

此外，有内胚层的标的基因表现在体外胰脏诱导分化过程的早期阶段，显示这些细胞为与内胚层相似的细胞。

The endoderm cells in the induction group were numerous with polygon shape, while in the control group were small amount of long fusiform.

加入全反式维甲酸的诱导组内胚层细胞呈多边形且数量较多，对照组内胚层细胞呈长梭形且数量少。

The mean somatotype data of male was 6.4-2.7-3.0, which focused on balanced endomorphy. The mean somatotype data of female was 4.8-2.3-3.0, focused on ectomorphic endomorph.

青岛高校男性大学生平均体型值为6.4-2.7-3.0，属于均衡的内胚层体型；女性平均体型值为4.8-2.3-3.0，属于偏外胚层的内胚层体型。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。