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METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h,MTT法检测ox-LDL对RAW264.7细胞生长的影响;流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡;用特异性siRNA沉默Daxx在RAW264.7细胞中的表达,通过AO/EB染色观察基因沉默后的细胞凋亡形态学改变;高效液相色谱检测细胞内胆固醇含量;油红O染色观察细胞内脂滴的形成情况;Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况;用Western Blot印迹法检测caveolin-1蛋白的表达。

Familiar location of trauma is shaft of tibia and fibula, shaft of thigh, shaft of ulna and ridius, ankle and foot, chest, knee, coax, pelvis, wrist and hand, shoulder, skull, shoulder bone, spine and elbow in turn. The familiar complication and accompanying trauma is shock, trauma of skull and brain, trauma of chest, trauma of nerve and vessel , fat embolism, trauma of organ in abdomen and trauma of urine system. 3. Emphasizing the treatment before and after the hospital can decrease the mortality and improve the quality of treatment. 4 The effect of early treatment is better than delayed treatment , internal fixation is done in 72h is better than after 72h, which can improve the function and reduce the complication menacing life.

常见的并发症和合并伤依次为:休克、颅脑损伤、胸部损伤、神经血管损伤、脂肪栓塞、腹部脏器损伤和泌尿系损伤。3、多发性骨与关节损伤的治疗以早期内固定效果为好,72h内手术并进行早期功能锻炼的患者远期功能恢第一军医大学2004届硕士学位研究生毕业论文复情况优于超过上述时限手术的患者。4、重视院前急救及院内各科室的急诊救治工作,有效的降低了死亡率,提供了早期处理骨关节损伤的良机。5、早期内固定治疗便于早期开展功能锻炼,可以明显改善伤肢功能,减少了威胁生命的并发症。

Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后,采用油红&O&染色,观察细胞内的脂滴,运用液体闪烁计数器检测细胞内胆固醇流出,高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量,用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

GA treatment restrained the respiration rate and ethylene production. 100mg.L-1ABA and 1000 L.L-1 ethephon evidently increased TSS and total titratable acidity acid content of green-mature fruit. The effect of ABA was more significantly than 1000 L .L-1ethephon, this suggested'ABA was the main hormone of influencing scenescence of'brumal jujube'fruit.

白熟冬枣采后冷藏过程中,内源ABA含量逐渐上升,贮藏后期有峰值出现;IAA和GA含量呈逐渐下降趋势;乙烯利和ABA处理都促进了内源ABA的积累和IAA和GA含量的下降,而ABA的作用比乙烯利明显;说明ABA调控果实的衰老是通过影响内源激素的变化而起作用的。

Performance prediction of the pump when transporting water and salt solution is also studied.Results are compared and analyzed with experimental data.6.The salt-out flow field is measured on three dimensions by Particle Image Velocimemter via radial and axial two test models.After image processing, velocity distribution of crystal particles in the pump is obtained.Particle flow field is visualized to reveal the particle motion law intuitionally.

此外对泵送清水和盐溶液时的性能也进行了预测,并将结果与外特性试验数据对比。6、运用PIV在径向和轴向两种测量模式下对泵内不同外部条件下盐析两相流场进行了准三维测量,经图像后处理,获得了盐析晶体颗粒在叶轮内、无叶腔中及轴面上的速度矢量分布,实现了颗粒流场的可视化,更为直观地揭示了泵内盐析颗粒的运动规律。

Results: All of 19 patients who received simple insertion and regular change of ureter stents experienced consistent improved renal function. Of 5 patients treated with antegrache or retrograde endoscopic ureterotomy/dilation and long-term indwelling of Di stent, 4 showed recovered and stable renal function, and 1 changed to nephrostomy because of unsatisfying outcomes. Three patients received permenant nephrostomy after unsuccessful endoscopic attempts and remained functionally uneventful.

结果:19例单纯输尿管内插入内支架管并定期更换,肾功能改善并基本保持稳定。5例采用顺行或逆行的输尿管镜内切开和/或扩张治疗,并长期停留双J管,4例肾功能有所恢复并稳定,1例效果不理想,改行肾造瘘。3例试行窥镜处理失败,改行长期肾造瘘,肾功能保持稳定。

The dynamo runs at rull speed to drive main shaft,then the main shaft makes the emulsifying wings in emulsification grove rotate,which absorbs the liquid in its brand groove into ring groove through guide vanes on upper and lower side s of the groove.The v

由电动机全速运转带动主轴,经主轴转动乳化框槽内之乳化翼片,使其将桶槽内之液体经框槽上、下两侧之导流叶片吸入环状框槽内,经以主轴为中心高速圆周旋转叶片;连续使乳化翼端刀面与乳化框间极小之间系高频率之强力切割剪断、粉碎,再借叶片高速离心旋转排挤,持续循环混合,以极短之时间即可完成均质乳化之处理。

Internally focused" means that victims tend to focus on their emotions when being harassed, not directly fighting back at the harassers. There are two types of internally focused victims: the one who is unaware of itand the one who is aware of it."

所谓「内隐型」的回应模式是指受害者对於性骚扰的回应偏向於处理自己的情绪,属於不直接对抗骚扰者的回应模式,其中又可以分为「未察觉内隐型」及「察觉内隐型」两种。

Under conventional N fertilization and irrigation condition, N2O emissions during 2001-2002 and 2002-2003 in winter wheat and summer maize rotation system were 1959 g N hm-2 and 1250 g N hm'2, respectively; and 68%-86% of N2O emission occurred in summer maize growth season, the emissions mainly occurred in 2 weeks after N fertilization and irrigation. The highest flux rates were 5.06 mg m-2 d-1 and 5.60 mg m-2 d-1 in the rotation period of 2001-2002 and 2002-2003, which were happened at the ten-extended leaf stage in summer maize season when top-dressing N fertilizer was applied.

在冬小麦/夏玉米轮作体系农民传统的水肥管理处理中,2001-2002和2002-2003两个轮作周期内测得的N_2O的排放量分别为1959 gN hm~(-2)和1250 gN hm~(-2),N_2O的排放主要集中在每次施肥和灌水之后的两周内,其中夏玉米十展叶期追肥后两周内N_2O的排放速率最高,2002年和2003年分别达到5.06 mg m~(-2)d~(-1)和5.60 mg m~(-2)d~(-1)。

The treating plant mainly has the following input signal in this set of control system: Che Neinei was mad that outside the temperature signal, the vehicle the external Qi temperature signal, the water temperature temperature signal, the air blower feedback signal, the mix air throttle feedback signal, the back light lamp signal and so on, the output device is the VFD fluorescence vacuum display monitor, the control device is mainly on control panel''s pressed key, realizes the plan acts according to the vehicle speed, Each temperature value''s situation comes the intelligent control air-conditioning system various air throttles electrical machinery and compressor''s work, causes in compartment''s temperature basis hypothesis temperature in the physical sensation comfortable scope, simultaneously controls the blowing through the air throttle electrical machinery''s movement the direction, the cold hot blast proportion, the automatic control, forces to defrost, the inside and outside circulates the wind transformation and the breakdown situation demonstration is the concrete function which this set of control system can realize.

在这套控制系统中处理装置主要有以下输入信号:车内内气温度信号、车外外气温度信号、水温温度信号、风机反馈信号、混合风门反馈信号、背光灯信号等,输出装置为VFD荧光真空显示屏,控制装置主要是控制面板上的按键,实现的方案是根据车速,各个温度值的情况来智能控制空调系统各风门电机和压缩机的工作,使得车厢内的温度根据设定温度在人体感觉舒适的范围之内,同时通过风门电机的动作来控制吹风的方向、冷热风的比例,自动控制,强制除霜,内外循环风的转换和故障情况的显示都是这套控制系统所能够实现的具体功能。

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