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Methods (1)Speciments of periphral blood were collected from 25 patients with JOAG ,their 10 relatives and 25 normal subjects. Polymerase chain reactionwas usd to analyze the sequence of MYOC gene consisting of all 3 exons and adjacent non-coding regions.

(1)应用聚合酶链反应方法,对25例开角型青光眼患者及其10例一级亲属和20例正常对照者的MYOC基因的3个外显子、部分内含子及部分启动子(7对引物)的各个片段进行扩增。

Pure precious essence complex richly contains needed fatty acid and vitamin B5 and E oil, offer your hair power of nutrition, moisture and soft texture.

纯正的珍贵精华油合成物,内含丰富纯天然的必需性脂肪酸和维他命 B5及 E 的滋润油,发挥特强的营养滋润,保湿和柔顺力量。

RT-PCR was used to detect the expressions of COL1A1 mRNA in 20 patients with idiopathic congenital talipes equinovarus.

采用半定量RT-PCR方法检测20例单纯性马蹄内翻足患儿下肢肌肉及肌腱组织中COL1A1基因mRNA的表达,根据COL1A1基因转录调控区-1 031 bp~+30 bp及第1内含子的序列,设计8对引物, PCR扩增后,采用变性梯度凝胶电泳技术筛查突变并测序。

Modern medicine confirmed that the B-Kudingcha acid-containing resin alcohol,蛇麻lipid alcohol, wood Kok,桷Paper-natural selenium-containing amino acids, such as more than 250 kinds of health medicinal substances, for its flavonoid content This tea味苦, sexual-ping, is the bitter food treasures (Fresh Five, are beneficial to the human body, generally at least to the bitter water, and its role is great).

现代医学证实,苦丁茶内含果酸B—香树脂醇、蛇麻脂醇、木角、桷皮素、天然硒化含物、氨基酸等250多种保健药用物质,其黄酮含量为绿茶的1.6~14.7倍,而咖啡碱含量仅为绿茶的1%。本茶味苦、性平,是苦味食品中的珍品(人食五味,皆有益于人体,一般以苦味食之最少,而其作用却很大)。

Results The tissue of 3 cases of ACC in the esophagus was mainly composed of square shape gland epithelial cell and variant myoepithelial cell, mesh shape cavity crack and the tubular, the entity shape arrangement, the cavity crack content basophilia and acidophilia the secretion. There was transmigration of superficial epidermis in 1 case of ACC; All 3 cases were positive of CK, H-CK, P63, S-100 (endochylema /nucleus), EMA, and Bcl-2. Case 3 was positive of CerbB-2 and P53. Case 2 was CD117, AAT, SMA, PAS/AB.

结果 3例食管腺样囊性癌的癌组织主要由立方形腺上皮细胞和变异的肌上皮细胞组成,呈筛孔状腔隙、管状、实体状排列,腔隙内含嗜碱性和嗜伊红分泌物;3例食管腺样囊性癌中例1表面上皮有移行;免疫组化:3例CK、H-CK、P63、S-100、EMA、Bcl-2均,例3 CerBb-2,P53,例2 CD117,AAT、SMA,PAS/AB。

Intercomparison.Sequence from Intronl to Exon2 fragment amplification by primers 2 of two individual selection from each swinery with pig L-FABP gene sequence published in GenBank,found 7 polymorphism loci existence in Individual.

引物2扩增的部分内含子1到外显子2片段每个猪群选择两个个体经测序得到的序列与GenBank上已发表的猪(DQ182323)的L-FABP基因序列比对,发现所测个体间存在7个多态位点。

Acini contain a small amount of secretions, but few have amyloid precursor.

腺泡内含有少量分泌物,但很少有淀粉样体。

University of Tokyo , Tokyo 11328657 , JapanAbstract : The genome DNA was extracted from the blood of six horse breeds including SH , B H ,XN , WZ , TB , AI. Four pair of primers were designed based on the sequence of human , mouse and ferret DRD4 gene cDNA to amplify the corresponding horse gene. The PCR products were ligated into a linear plasmid pMD17 , and sequenced respectively.

从6 个品种马的血液中提取基因组DNA ,根据GenBank中已发表的人、鼠、雪貂的DRD4 cDNA 基因保守区序列,设计3 对特异性引物,另1 对由文献所得,对6个品种马的DRD4 基因分4 段进行PCR 扩增,经电泳检测,分别呈4条特异条带,将其分别克隆入pMD192T 载体中,进行序列测定并拼接序列,得到包括所有内含子的DRD4 基因序列。

Results: After cryofixation,basement membranes of skeletal muscle consisted of only one electron dense layer, T tubules were round, core cylinders were observed in terminal cisternae and there were thread-like protein particles on the membranes of terminal cisternae.

结果:冷冻固定后,缝匠肌基膜仅仅由电子密度高的一层组成;横小管为圆形;终池内含有电子密度高的环形物,终池膜上存在一排线状电子密度高的蛋白颗粒。

Two phylogenetic footprints, 35 bp and 17 bp in length respectively, were found in 5' intron sequence.

在内含子的5'端发现了两个高度保守的序列——进化印记,长度分别为35 bp和17 bp,35 bp的核苷酸片段能与HeLa细胞和鸡胚细胞抽提物中的蛋白结合,可能是一种调节元件。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。