内含物

Common buckwheat ( F. esculentum ) is an allogamy crop, which is heterogony. There is heterogeneity in the species. To reveal the genetic diversity within cultivar population, In order to explore the sampling strategy that can reveal genetic diversity of buckwheat resources on the molecular level. PCR with intron-splice junction primers and long random primers was employed to reveal the genetic diversity within nine common buckwheat varieties and one tartary buckwheat cultivar population among different individuals. We established the sampling strategy in its genetic diversity study. Then we used the established sampling strategy, and the same PCR markers technique,to analysis the genetic diversity of 86 common buckwheat resources, the main results are as follows

甜荞是花柱异长自交不亲和的异花授粉作物，品种内存在一定的异质性，为了准确揭示甜荞遗传资源的遗传多样性，本研究采用因子分析法对86份甜荞遗传资源农艺性状数据进行分析；采用长随机引物和内含子切接点引物的PCR分子标记技术，对9个甜荞品种和1个苦荞品种不同个体间的遗传多样性进行了分析，建立了甜荞资源遗传多样性研究的取样策略；并利用建立的取样策略，采用PCR标记技术，对这86份甜荞资源的遗传多样性进行了分析，取得如下主要结果

However, in human congenital system proteins associated with BSP in function have not been reported up to date.

此外，由于内含子序列的存在，因此扩增结果与引物设计的部位直接有关，选用3′UTR部位的引物为好。

The present invention relates to a rubber composition including 100 parts by mass of a rubber component including a natural rubber and/or a diene rubber and 0.5 to 80 parts by mass of a biodegradable aliphatic polyester.

本发明涉及相对于含有天然橡胶及/或二烯烃系橡胶的100质量份橡胶成分，进而在0.5～80质量份的范围内含有生物降解性脂肪族聚酯的橡胶组合物。

Fatty acid desaturase gene was cloned from genomic DNA and cDNA of Euglena gracilis through PCR with primer designed acroding to the reported equences.This gene had an open reading frame of 1266bp that encodes 421 amino acids.

根据已报道的Δ8-脂肪酸脱氢酶基因设计引物，分别从小眼虫藻基因组DNA和cDNA中扩增得到该基因片段，序列分析结果表明，结构基因长1266bp，编码421氨基酸，而且没有内含子，这是国内外首次报道。

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞：弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等；腺上皮下的基底膜区有少量结缔组织存在；扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况；透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞；近基底膜区观察到富含粗面内质网的细胞；基底膜间为薄层疏松结缔组织，内含肌细胞及神经末梢等结构。

The results showed:intercomparison sequence from Exonl to part Intronl fragment amplification by primers 1 of two individual selection from each swinery with pig L-FABP gene sequence published in GenBank,found that base site of 237、258、315 of all individuals were A、T and A respectively,different with DQ182323 base sequence of G、C and C,and existence G403A mutation between individual.

结果表明：引物1扩增的外显子1到部分内含子1片段每个猪群选择两个个体经测序得到的序列与GenBank上已发表的猪(DQ182323)的L-FABP基因序列比对，发现所测所有个体237位点、258位点和315位点碱基分别为A、T和T与DQ182323序列的碱基G、C和C不同，而且所测个体间存在G403A突变。

The introduction combines traditional Chinese and Western medicine below the experience of enteritis of noxiousness of fine ailment of remedial Tibet mastiff, in order to offer reference. 1 hair sick condition and clinical expression ill dog are 2~4 month more age young dog, majority is come on suddenly, anorexia or abandon absolutely, mental depressed, systemic symptom worsens quickly, produce acuteness sex vomiting and diarrhoea, puke is first feed rotten, afterwards is yellow or olive bubble mucus and hematic type thing, have diarrhoea dung has the sticky stiff thing of grey yellow, turn after for rare dung of effluvial shape of embedded mucous membrane, then shows hematic dysentery, because ill dog is acuteness vomiting and diarrhoea are rapid dehydration, eyeball cave in, temperature is shown two-way and calorific,℃ of the 40~41 at the beginning of disease, 1~2d falls it is normal to come, after 3~4d answer elevatory, die very quickly next, 4~5d of course of diseases, the temperature when on one's deathbed drops more to normal temperature the following. Bottom of stomach of dog of die in one's bed of 2 analyse check has haemorrhage sex inflammation, show cardinal, small intestine mucous membrane falls off, alvine wall attenuates, there is gules mucus inside, mix inside large intestine have show wine blood excrement and urine, there are a lot of haemorrhage places on mucous membrane, haemorrhage of rectum mucous membrane is more, mesentery lymph node enlargement, sometimes afterwards sends intussusception, alvine tangent plane bleeds, cardiac muscle is loose, color becomes weak, cystic plentiful, liver is qualitative fragile and brittle. 3 diagnose 3.1 epidemiology to diagnose this ill much hair at young dog, rather dog of epidemic disease have an inoculation feels the most easily, be affected directly or affect secondhand, basically pass enteron infection, this disease all can happen all the year round, but with cold...

下面介绍中西医结合治疗藏獒细小病毒性肠炎的心得，以供参考。1发病情况和临床表现病犬多为2~4月龄幼犬，多数为忽然发病，食欲减退或废绝，精神沉郁，全身症状急剧恶化，发生剧烈性呕吐和腹泻，呕吐物先为食糜，继为黄色或黄绿色泡沫黏液和血样物，泻粪有灰黄色的黏稠物，后转为恶臭的内含黏膜状稀粪，继而呈血痢，由于病犬剧烈呕吐和腹泻迅速脱水，眼球下陷，体温呈双向发热，病初40~41℃，1~2d降至正常，3~4d后又复升高，然后很快死亡，病程4~5d，临死时体温多下降至常温以下。2剖检病死犬胃底部有出血性炎症，呈深红色，小肠黏膜脱落，肠壁变薄，内有红色黏液，大肠内混有呈暗红色血液粪便，黏膜上有许多出血点，直肠黏膜出血较多，肠系膜淋巴结肿大，有时继发肠套叠，肠切面出血，心肌松软，颜色变淡，胆囊充盈，肝质脆而易碎。3诊断3.1流行病学诊断本病多发于幼犬，未免疫接种犬最易感，直接感染或间接感染，主要通过消化道感染，该病一年四季均可发生，但以寒。。。

Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.

通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对，在两端外显子上分别设计上下游引物：Psf：5'CAAGGATGAGAAACTGTGCAA3'；Psr：5'GAATATCTTCTGCTCCTACTG3'；再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因，最后经过10％聚丙烯酰胺凝胶电泳、银染，根据带型判断性别，其中雌性为两条带，雄性为一条带。

Leucorrhea women is vaginal mucosa exudate, cervical and endometrial gland secretions of a mixture containing Bacillus vaginal and genital tract mucosa of exfoliated cells, the amount of leukorrhea and the traits and the level of estrogen-related.

妇女的白带是阴道粘膜的渗出物、宫颈腺体及子宫内膜的分泌物混合而成，内含阴道杆菌及生殖道粘膜的脱落细胞，白带的量及性状与雌激素水平的高低有关。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了