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On the basis of sequences from GenBank,part of exonl and intronl of goat Agouti gene fragments was amplified.

本试验根据GenBank上已发表的牛(X99691)、猪(AJ427478)马(AF288358)和人的Agouti基因序列设计引物,扩增山羊Agouti基因外显子1和内含子1的部分序列片断,内含子1的部分序列与本实验室之前测得的山羊Agouti基因序列片断(GenBank序列号:EF587236)进行拼接,得到5270bp长度的山羊Agouti基因序列。

The whole analysis was completed on a C〓 column in 18min at a flow rate of 0.5 ml/min, with the mobile phase of methanol-water (93: 7, v: v) containing 75 mmol/L TEOLA and 200 mmol/L HAc, or with the mobile phase of ethanol-water (90: 10, v: v) containing 25 mmol/L TEOLA and 200mmol/L HAc pumped at 0.25ml/min. Compared with the methanol system, the ethanol system was a better candidate for the separation of thulium porphyrin complexes in view of selectivity.

在C〓分析柱(5μm,2.5×150mm)上,流动相为:甲醇:水=93:7(v/v内含75mmol/L三乙醇胺和200mmol/LHAc),流速为0.5ml/min和流动相为:乙醇:水=90:10(v/v,内含25mmol/L三乙醇胺和200mmol/L HAc),流速为0.25ml/min,均能使六个铥卟啉配合物在18min内得到有效的分离,相比之下,乙醇体系对铥卟啉配合物更具有分离选择性。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建:设计引物,通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70/5UTR)、HSP70基因3&UTR序列(HSP70/3UTR)及β-微管蛋白基因内含子C序列,通过酶切连接,构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的,以β-微管蛋白基因内含子C序列作为间隔序列,以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70/5UTR-IntronC-HSP70/3UTR,序列测定结果正确。

The contents are often digested by enzymes from lysosomes.

内含物通常被溶酶体的酶消化。

Tannin inclusions in vacuoles have also been demonstrated by the electron microscope.

液泡中的丹宁内含物也已用显微镜证实。

OFTEN SEEN IN INCLUSIONS OF SILICATES SUCH AS GARNET, WHOSE CRYSTALLOGRAPHY

内含物中,而这类物质的晶体结构普遍地在某种程度上类似于金刚石的晶体结构。

It is not known how rare this resemblance is,or whether it is most often seen in inclusions of silicates such as garnet,whose crystallography is generally somewhat similar to that of diamond;?

现在尚不知这种类似稀少到何种地步,也不知道是否它最常见于象石榴石一类的硅酸盐内含物中,而这类物质的晶体结构普遍地在某种程度上类似于金刚石的晶体结构。

It is not known how rare this resemblance is,or whether it is most often seen in inclusions of silicates such as garnet,whose crystallography is generally somewhat similar to that of diamond; but when present,the resemblance is regarded as compelling evidence that the diamonds and

现在尚不知这种类似稀少到何种地步,也不知道是否它最常见于象石榴石一类的酸盐内含物中,而这类物质的晶体结构普遍地在某种程度上类似于金刚石的晶体结构。

Of thick bark usually located in windward habitats. The phloem color was related to the inclusion and contents of the phloem cells, especially to the tannin and resin of the phloem parenchyma and their oxidation.

但是韧皮部的颜色则与细胞内含物的多少和成分有关,尤其是韧皮薄壁细胞内的丹宁和树脂的含量以及它们在空气中的氧化作用有关。

A conventional abrasive mixture pressurising system comprises a pressure vessel (15), a nozzle (13) connected to said compartment, an inlet (12) for fluid under pressure to drive the contents of the compartment to the nozzle and a supply of abrasive mixture.

本发明对传统的磨料混合物供应系统作了改进。传统的磨料混合物增压系统包括一高压容器(15),一与所述室相连的喷嘴(13),一加压流体的入口(12)用以把该室的内含物运送到喷嘴,以及一磨料混合物供应源组成。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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