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The morphogenesis of floral organs in both srg mutant and wild-type rice Dular (Oryza sativa L.) was observed using the scanning electron microscope. The results showed that the floral primordia were differentiated under irregular pattern in srg mutant. After regular formation of lemma and palea primordia, the floral primordium of srg formed lodicule primordia inner to both lemma and palea, followed by the formation of stamens and carpel. Alternatively, floral primordia in srg were split to form two or more floral primordia. The number and position of stamens and carpels primordia were malformed.

利用扫描电子显微镜观察野生型水稻和裂颖水稻的颖花原基分化情况,结果发现,突变体水稻的幼穗在形成内、外稃原基后,有些颖花在形成浆片后并不是直接发育形成雄蕊原基,而是在外稃或内稃原基内侧又分化出浆片原基;有些颖花原基从中间缢裂,缢裂的颖花原基又分别形成类稃片的结构,颖花发育后期,6枚雄蕊发育畸形,并且观察到不同数目的雄蕊。

These results suggested that the lung Fib were activated and produced excess HA, resulting in the excessive accumulation of HA within the lung at the stage of alveolitis during the process of pulmonary fibrosis. However, increased HA synthesis of lung Fib and increased HA levels in BALF merely reflect the intensity of alveolitis and the disease activity rather than the degree of pulmonary fibrosis.

以上结果提示:肺Fib在肺纤维化的早期肺泡炎阶段就处于活化状态,产生HA增多,导致肺内HA的过多聚积,肺Fib产生HA增多和BALF中HA水平的增高可反映肺泡炎的强度和疾病的活动性,但并不能反映肺胶原代谢或纤维化的程度。

Objective To compare the changes of the number of the blood cell and coagulatory functions in the intracavitary blood before the use of Autologous Blood Recovery System and after.Methods The RBC,Hct,Plt,PT,APTT,FIB,TT of the intracavitary blood were measured in thirty-two patients with acute closing intraabdominal and intrathoracic hemorrhage.

目的 比较自体血液回收机处理后胸/腹腔内积血血细胞量和凝血功能变化的特点方法 32例急性闭合性胸/腹腔内出血病人,分别检测自体血液回收机处理前后体腔内积血的红细胞数、红细胞压积、血小板、凝血酶原时间、部分凝血酶原时间、纤维蛋白原、凝血酶时间。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示:未妊娠时,泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达,波形蛋白在子宫内膜基质细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,牦牛子宫任何部位均不表达角蛋白7;妊娠30天左右时,泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达,波形蛋白在子宫内膜基质细胞和内胚层细胞内表达,平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达,角蛋白7在尿囊细胞内表达,偶尔在腔上皮细胞的细胞核边缘表达;消化法进行原代培养时,组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞;分离得到的子宫内膜基质细胞活率达90%以上,并可在体外传代7次以上;分离得到的子宫内膜腺上皮细胞活率可达85%以上,并可在体外传代5次以上;RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长,维持子宫内膜基质细胞正常生长的FBS添加量为20%,维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富,软骨基质染色均匀,软骨细胞数目最多,Ⅱ型胶原蛋白表达最活跃,平均积分光密度值最高;青少年组软骨膜内血管减少,软骨基质染色出现明显的不均质状,软骨陷窝体积变大,并呈分隔状,陷窝内软骨细胞数目减少,II型胶原蛋白表达较儿童组减弱;成人组软骨膜血管、细胞成分明显减少,软骨膜内的纤维成分明显玻璃样变,钙盐沉积较青少年组时明显增多,Ⅱ型胶原蛋白表达较青少年组减弱。

Methods Constructed the model of fetal scarless wound healing and investigated the expression of collagen in fetal and adult rat wounds by immunohistochemistry and in situ hybridization.

染色,Ⅰ、Ⅲ型胶原免疫组化,Ⅰ、Ⅲ型胶原原位杂交。结果胎鼠伤口愈合快,组织结构再生完全,没有瘢痕形成,胶原呈网状正常排列,Ⅰ、Ⅲ型胶原蛋白及表达均增多,同时伴细胞数增多,Ⅲ型胶原首先形成网状排列;成年伤口胶原纤维沉积慢,平行排列的胶原逐渐呈束状填塞伤口以致瘢痕形成,伤口内Ⅰ、Ⅲ型前胶原mRNA表达均增多,细胞数目增多不明显。

There was a significant inverse correlation between sarcomere length and A/I (r=-0.686, P.05). 4 Relations of the skeletal muscle histological characteristics and meat quality The muscle fibre and the myofibrils diameter were thinner, the muscle fiber density was denser, the sarcomere length was longer and the meat color was redder. The A/I was bigger, the meat color was whiter, contrarily was redder. The right amount of fat content may improve the meat brightness. The muscle fiber diameter and the myofibrils diameter were the biggest factors to influence the skeletal muscle shearing force, their assumed the obvious linear correlation. The muscle fiber density influence on the shearing force was only inferior to the muscle fiber and myofibrils diameter. Mensurate the IFA from tectology angle may reflect in intramuscular fat content. However intramuscular fat was not enough to carry weight on the shearing force, it may mostly affected on the smack, but not the tenderness expression.

猪骨骼肌组织学特性与肉质性状的关系肌纤维和肌原纤维直径越小、肌纤维密度越大、肌节长度越长,肉色鲜红;肌节中暗带比例越大,肉色偏白,而明带比例大,肉色偏红;适量的脂肪含量可以改善肉的亮度;肌纤维直径和肌原纤维直径对骨骼肌剪切力的贡献率最大,呈明显的线性相关,肌纤维密度对剪切力的贡献率仅次与肌纤维和肌原纤维直径;从组织形态学角度测定肌内脂肪面积比一定程度上可以反映肌肉内的脂肪含量,而肌内脂肪对肌肉剪切力的贡献率比较微弱,对肉食用品质的影响可能更多表现在改善肉的风味,而不是嫩度的表达。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均,全层或局部区域显著变薄向外膨出,内皮细胞空泡变性或坏死脱落,部分内皮细胞剥离并突入管腔成游离状,可见血栓形成及部分血栓机化;内弹力板变薄、消失或突然中断;在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原;部分动脉瘤壁呈均质状玻璃样变,平滑肌细胞明显减少;中膜和外膜可见大量的炎性细胞浸润;瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞,胞浆内充满脂类物质及胆固醇结晶;部分动脉瘤腔内充满血栓,有的血拴已经纤维化,血栓基部机化,血栓表面有红细胞和纤维素。

Tang Jiamin Heritage Site is located at the city Department of Tang village seven kilometers, the original name for the Tang rich private house, built in the late Ming and early Qing Dynasty, there are a two-phase adjacent to the three homes and other houses of a total of five branches of the house, 100 50 I, the area of 1483 square meters; the salt controller Downtain Civil Mishina a tomb and a stone arch, its architecture combines the north courtyard and garden art Jervois combined in the brick, wood carving, stone carving exquisite, delicate, may be comparable to the National Palace Museum's sculpture, the garden, the roof ridge lying beast flying eaves hook tooth picks, intrigue, terrazzo floors and walls of brick, set with wood, brick, stone

唐家民居位于旬邑县城七公里处的唐家村,原为唐姓财主的私人宅院,修建于明末清初,现存有两进相毗连的三院和其它两院共五院房子,一百五十余间,面积1483平方米;有三品盐运使唐廷铨的陵墓一座和石牌坊一座,其建筑融北方四合院和苏杭园林艺术相结合,内中的砖雕、木雕、石雕精美、细腻,可与故宫博物院的雕刻相媲美,整个庭院,屋顶脊卧兽飞,檐牙勾啄,勾心斗角,墙壁为水磨石砖,镶以木、砖、石雕、造型优美,巧夺天工,至于门栏窗棱更是玲珑剔透,堪称为中国古代建筑艺术的典范。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果:①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后,逐渐呈灰黄色,表面粗糙,出现裂隙;当压力逐渐减小后,变黄、粗糙、有裂隙的软骨颜色逐渐恢复,变得有光泽②光镜下可见表面光滑、平整,细胞分布均匀、整齐,层次清楚的软骨在关节面压力增大后,表面变粗糙、缺损,细胞排列紊乱、染色不均;当关节面压力逐渐减小后,软骨表面逐渐修复,细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达,术后3周内表达逐渐变弱,从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后,软骨细胞逐渐变扁,胞质中内质网膜、高尔基体减少,胶原排列紊乱;当关节面压力减小,软骨细胞形态逐渐恢复正常,胞质中内质网膜、高尔基体数量逐渐恢复;胶原纤维排列逐渐有规则。

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