内原的

Methods By establishing the apoptosis model of primary culture of hippocampal neurons induced by hyperthermia, the dynamic change of concentration Ca(superscript 2+)in hippocampal neurons and the influence to Bcl-2 expression were observed after using chelerythrine chloride which was the special inhibitor of PKC.

通过建立体外高温诱导海马神经细胞原代培养的凋亡模型，应用PKC特异性抑制剂氯化百屈菜红碱，观察海马神经元胞内Ca(上标 2+)浓度的动态变化以及对B淋巴细胞瘤基因2蛋白(Bcl-2)表达的影响。

Jeffrey Garten, once under-secretary of commerce in charge of international trade and then dean of Yale School of Management, said that a keiretsu restrains trade "because there is a very strong preference to do business only with someone in that family".

原商务部副部长，现任耶鲁大学管理学院院长的杰弗里-戈登认为集团公司遏制了贸易&因为他们有只和集团内的公司做生意的强烈倾向&。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

In the rabbits, every Nd: YAG laser iridotomy smaller than 200μm and argon laser iridotomy, Nd: YAG laser combined with argon laser iridoto- my smaller than 450μm closed by the first postoperative week. The mecha- nism for iridotomy closure was assumed to be iris pigment epithelial prolifera- tion, migration and the reformation of stromal connective tissue once the pos- terior pigmented cells have initially bridged the iridotomy.

四、兔眼虹膜激光损伤后的修复能力较强，小于200μm的Nd：YAG激光孔及小于450μm的氩激光、Nd：YAG激光联合氩激光孔均在术后1周内关闭，前者以基质胶原结缔组织再生较明显，而后者以色素上皮增殖、迁移为著。

Messina were plenty busy this summer signing players from across the world. Their new additions include players from Italy like Nicolas Cordova, Daniele De Vezze, Massimo Minetti, Antonio Ghomsi, Rok Straus, Edgar Alvarez and Angelo Rea. In addition to French player Lavecchia, Mark Iuliano (who was capped for Italy national football team in Euro 2000 and FIFA World Cup 2002) and last but not least Japanese player Mitsuo Ogasarawa from Kashima Antlers.

梅西纳这个夏天非常忙碌的满世界大肆挖人，他们新签球员包括科尔多瓦、德微泽、米内蒂、贡西、斯特劳斯、阿尔瓦雷斯和雷亚，除此之外还签入了法国球员拉维奇亚、尤里亚诺（参加过2000年欧锦赛和2002年世界杯的意大利国脚）还有从鹿岛鹿角加盟的前日本国脚小笠原满男。

The initial threshold could be chosen by using Otsu algorithm, and in the iteration process, an optimal threshold was determined by maximizing the MI between the original image and the segmented image.The effectiveness of the proposed approach was evaluated by applying it to the microphotographic.

首先利用Otsu算法确定全局阈值作为初值，以互信息量为目标函数，在小范围内计算分割图像与原图像的互信息量，互信息量达到最大时的阈值即为最优值，这是将图像配准方法用于分割的一种创新性尝试。

The product contains recombinant human bone morphogenic protein in a collagen scaffold. The protein promotes differentiation of cells into bone-producing osteoblasts when implanted into target areas of the spine.

该产品为一胶原支架，内含重组的人类促骨生成蛋白；当移植入脊椎的目标区时，此蛋白可以促进细胞分化至制造骨质的成骨细胞内。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

One big change, says Doug Paal, a senior China adviser to previous American presidents and now at the Carnegie Endowment in Washington, DC, is that the growth of the internet generation in China nowadays requires its government to heed the strong nationalist sentiment of its own people.

原任职于美国总统手下，现任职于卡内基基金会的中国问题高级顾问道格。帕尔认为，有一个巨大变化是，如今中国网络一族的壮大要求中国政府得留意自己国民的强烈民族情绪。

Methods The 201 cases in 1979～1996 were retrospectively summarized. The clinical features including chief complaints, sex distribution, age at first visit, laterality, type of presentation, ocular deviation in the primary position, refractive errors, amblyopia, globe retraction, change of the palpebral fissure, upshoot and downshoot in adduction, binocular single vision, and its associated ocular and non-ocular anomalies were analyzed.

自1979～1996年回顾性地总结了201例DRS，对它的临床特征，包括主诉、性别分布、初诊年龄、侧向状态、表现类型、原在位的眼位偏斜、屈光不正、弱视、眼球后退、睑裂改变、内转时眼球急速上转和下转、双眼单视及其伴随的眼部和非眼部异常等予以分析。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。