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At last instar nymph and after flying stage, c-fos immunoreactivity of procerebrum was weak, but the female and male termites displayed significantly increased number of c-fos labeled cells in the protocerebrum at flying stage.

本研究首次证实白蚁婚飞行为与其前脑部神经活动有密切关系;原癌基因c-fos在调节与性行为有关的前脑神经细胞核内基因表达方面有重要作用。

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下:在成熟卵细胞中多聚核糖体数量不多,且细胞代谢活性较弱;初期合子内,核仁大量合成核糖体前体物质,胞质中多聚核糖体数目众多,细胞代谢活性较强;休眠期合子的核仁变小,胞质中核糖体数量急剧减少,仅有少量多聚核糖体,细胞代谢活性较弱;合子分裂前期和二细胞原胚期,核仁显著,胞质中核糖体的密度增加,出现大量多聚核糖体,细胞代谢活性较强。

Drugs inhibiting collagen hydroxylation and accumulation are expected to improe this situation, making prolyl 4-hydroxylase (P4H), the key enzyme of intracellular collagen processing, a rational target for pharmacological interention.

抑制胶原羟化和沉积的药物被临床医生所期盼,生成的羟脯氨酸酶{P4H},肝内细胞胶原生成的关键酶,是药物介入的理想目标。

MethodsIn the solvent of 1 mol/L NaOH, using the reaction of the water-soluble phenolic acidity in Radix Salviae Miltiorrhizae and the NaNO_2-Al(NO_3)_3, the total components of water-soluble phenolic acidity in Radix Salviae Miltiorrhizae injections and drip solution(calculated with protocatechualdehyde) were determined by colorimetric s...

方法利用丹参中水溶性酚酸类成分与亚硝酸钠和硝酸铝溶液在碱性条件下的显色反应,比色法测定该两种制剂中水溶性酚酸的总量。结果原儿茶醛在3.336~14.46mg/L范围内呈良好的线性关系,回归方程为:A=0.02777C+0.01279(r=0.9996),加样平均回收率为100.8%,RSD为0.96%。结论该法简便快速,结果准确,重现性好,可用于该制剂的质量控制。

Methodsa gradient hplc system was used in the quantitative analysis of the danshensu ,protocatechuic aldehyde and salvianolic acid b in 6 hours,and its ph was determined at 25℃for 24 hours.

方法采用高效液相色谱法梯度洗脱测定配伍液中6 h内丹参素、原儿茶醛、丹酚酸b的含量。并在25℃时24 h测定配伍液的ph值。

Results Protocatechuic acid has linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系;平均回收率为97.76%,RSD=1.01%。

Methods:The RP-HPLC method was applied and protocatechuic aldehyde was used as the internal standard substance.

采用RP-HPLC法,以原儿茶醛为内参照物,色谱柱:Luna C_(18)柱(250mm×4.6mm,5μm),流动相:0.05%磷酸水溶液-乙腈,梯度洗脱程序为:0min时A-B(100∶0),80min时A-B(67∶33),85min时A-B(60∶40),100min时A-B(60∶40),流速:1.0mL·min~(-1),检测波长:280nm。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系;平均回收率为97.76%,RSD=1.01%。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

效果 原儿茶酸在0.103~0.7725 μg领域内呈良好的线关连;均匀接纳率为97.76%,RSD=1.01%。

These results also provedthe presence of the same acute phase response profile in amphioxus,as observed ineutherians.Thus it pushed the evolutionary existence of the eutherian-like acute phaseresponse back to the protochordate lineage.Fourthly,we determined CAs,including epinephrine、norepinephrin anddopamine,with HPLC method optimized after LPS challenged amphioxus.Theresults demonstrated that E,DE and DA are present in amphioxus humoral fluid,andE was firstly found in amphioxus.

第三,我们用生物化学方法,选取海洋中广泛存在、众多水产动物的常见致病菌-溶藻弧菌作为天然反应原感染文昌鱼,从另外一个角度探讨并丰富文昌鱼体液中的八种炎性蛋白的急性时相反应,结果显示,与LPS感染文昌鱼相比,六种正APPs的浓度变化幅度相对较小,曲线相对平缓,但仍然呈正急性时相反应,蛋白电泳也证实了这一点;而二种负APPs:RBP和白蛋白(Albumin,ALB)浓度实验周期内总体呈下降的趋势,呈负急性时相反应,结论与上述LPS相同。

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