内切

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

Objective To establish an initial system of identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene.

目的 初步建立我国致病性钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类体系。

Objective To establish an initial system of identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene.

目的初步建立我国致病性钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类体系。

Restriction sites polymorphisms of rRNA gene for Leptospira can be used to classify and identify Leptospira in China and also to study on molecular epidemiology for leptospirosis.

rRNA基因限制性内切酶酶切位点多态性可用于钩体分类，而且是钩体病分子流行病学调查的一种好方法。

The restriction enzyme patterns for eight species of mycobacteria were studied.

对PCR扩增片段的酶切样品的预处理和CE条件进行了优化，获得了8种分枝杆菌DNA的限制性内切酶谱图。

Among 90 positive clones in the 16S rDNA library of microorganisms, 23 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, delta subclasses of the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria and Planctomycetes.

采用限制性内切酶HaeIII和RsaI对克隆文库中的90个克隆子进行了酶切分型，根据ARDRA酶切图谱的不同，可将其分为23个OTUs.16S rDNA序列分析结果表明，该克隆文库中主要包括变形菌门的alpha、beta、detla亚类、厚壁菌门、放线菌门、拟杆菌门、酸杆菌门及浮霉菌门等8类细菌及未培养细菌。

Improved high ionic strength medium was adopted to extract the cpDNAs of Yunnan wheat,Tibet semiwild wheat and two cultivars of common wheat (Chinese Spring and Een 1).Restriction fragment analysis of cpDNAs was carried out with the use of 7 restriction enzymes.

亚种。我们采用改进的高离子强度法，提取云南小麦、西藏半野生小麦、普通小麦的两个品种(中国春和鄂恩1号)的叶绿体DNA，并用7种限制性内切酶对它们的叶绿体DNA进行了酶切图谱分析。

In the west of China.Improved high ionic strength medium was adopted to extract the cpDNAs of Yunnan wheat,Tibet semiwild wheat and two cultivars of common wheat (Chinese Spring and Een 1).Restriction fragment analysis of cpDNAs was carried out with the use of 7 restriction enzymes.

亚种。我们采用改进的高离子强度法，提取云南小麦、西藏半野生小麦、普通小麦的两个品种(中国春和鄂恩1号)的叶绿体DNA，并用7种限制性内切酶对它们的叶绿体DNA进行了酶切图谱分析。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

An enzyme that catalyzes the hydrolysis of a nucleotide to a nucleoside and phosphoric acid .

核酸酶水解核酸的酶，如核酸内切酶和核酸外切酶

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。