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Methods and materials A549 cells were collected and lysed for total protein after treated with metformin (5 mM) for 0, 15, 30 and 60 minutes. Western blot analysis was performed to detect the expressions of pan- and pho- AMPK, JNK and p38 proteins. Cleaved Caspase-3, 8, 9 proteins were also detected.

以5mmol/L的二甲双胍对A549细胞进行体外干预,在干预的第0、15、30和60min时,分别抽提细胞总蛋白,以Western blot技术检测细胞内AMPK、JNK、p38 MAPK的总蛋白和磷酸化蛋白的表达;在干预后第0、12、24和48h,检测剪切后的Caspase-3、8、9活性蛋白的表达水平。

A conceited German is the worst of them all, and the most hardened of all, and the most repulsive of all; for he imagines that he possesses the truth in a science of his own invention, which is to him absolute truth.

他与安德烈公爵和切尔内绍夫说了几句关于当前战争的话,他的神情仿佛在说,我早就知道一切都会弄糟的,甚至对此抱有得意之色,那脑后一撮撮翘起的头发和匆匆梳过的鬓角都说明了这点。

Properties of these samples are tested by FAST instrument, including bending rigidity, extensibility, cutting resistance and seam puckers at different directions, and subjective evaluations on seam pucker are carried out in accordance with the related standard of AATCC.

在0°~360°范围内沿面料经向每隔10°分别取样并缝制,还包括45°、135°、225°和315°斜向角度,用FAST风格仪测定不同方向面料的弯曲性能、拉伸性能、剪切性能,分别测定面料不同方向的缝缩率,并通过AATCC标准对缝缩进行主观评价。

Mascherano had already been booked for scything through Paul Scholes when he was shown a second yellow card for dissent after questioning Bennett's decision to book Fernando Torres.

马斯切拉诺已经因为对斯科尔斯的铲球犯规拿了黄牌,还由于质问本内特对托雷斯出示黄牌再次吃牌从而被罚下。

Joey Chestnut, from California, inhaled 66 hot dogs in 12 minutes, shooing past six-time winner, Takeru Kobayashi from Japan.

来自加州的切斯纳斯特在12分钟内吃了66个热狗,成功从来自日本的六连胜小林尊手上夺去冠军腰带。

Joey Chestnut, from California, inhaled 66 hot dogs in 12 minutes, shooing past six-time winner, Takeru Kobayashi from Japan.

来自加利福尼亚州的乔伊·切斯特纳特在12分钟内鲸吞66个热狗,击败了来自日本曾经六次夺冠的小林尊

Safehands For the second time in two minutes, Cech rises high and snuffs out aerial danger.9 P c/~$ V9 _- y8 p!

119分–可靠:切赫高高跳起,两分钟内第二度扑灭了对方的高空攻势。

From about 206 Ma there occurred arc-continent collision accompanied by compressional contraction and shearing deformation of the arc crust and development of syn-collision granites. In the late Yanshanian epoch (138-73 Ma) post-orogenic extension came into action and resulted in the generation of A-type granites. During the Himalayan (65-15 Ma) intra-continental orogenic movement a large-scale strike-slip thrusting induced the intrusion of granites and development of pull-apart basins.

据此,再造了造山带的形成过程与演化历史:印支期的大规模俯冲造山作用(238~210 Ma),形成义敦火山岩浆弧;大约自206 Ma始,发生弧-陆碰撞,伴随岛弧地壳挤压收缩和剪切变形,发育同碰撞花岗岩;进入燕山晚期(138~73 Ma),岛弧碰撞造山带发生造山后伸展作用,形成A型花岗岩带;喜马拉雅期发生陆内造山作用(65~15 Ma),岛弧碰撞造山带出现逆冲-推覆和大规模走滑平移,伴随喜马拉雅期花岗岩的侵位和拉分盆地的形成。

Therefore, the face-width of the face gear is limited. According to the conditions of undercutting and pointing, the formulae of minimum inner diameter and maximum outer diameter are derived for the shaft angle face gear .

根据产生根切与齿顶变尖的条件,给出了任意轴交角下齿宽设计的公式,定义了两个无量纲参数,即最小内半径系数和最大外半径系数,来表示齿宽的设计范围。

Ma'anshan De'ermeng Heavy Industry Machinery Co., Ltd. was founded in 2003 which has introduced the United States DNYA vertical machining centers and CNC numerical control machine tools and owned mechanical processing, centrifugal casting, precision casting, forging, heat treatment and other production workshops etc.. Besides, our company is the IS09001: 2008 quality certification company and own sophisticated equipment and completely testing methods. The main products of our company include the series of machine tools , hydraulic notching machine, joint punching machine, metallurgical spare parts type, mill stick road, Cold-rolled straightening roller, wear and heat-resistant lining ,the furnace slip block, roller, reverse roller, pinch roller, rail beam correction circle, saw drilling machine spare parts, mid-thick plate, hot-rolled plate blade, vertical disk Scissors blades, and other demanding mechanical spare parts.

马鞍山德尔盟重工机械有限公司成立于2003年,是一家集工、贸于一体化的企业,长期与意大利、台湾地区技术合作,公司引进了美国DNYA立式加工中心及CNC数控机床,并拥有机械加工、离心铸造、精密铸造、锻造、热处理等生产车间,设备精良,检测手段齐全,系IS09001:2008质量认证企业,主要生产机床类、液压切角机、联合冲剪机、冶金备件类、轧机棍道、冷轧矫平辊、耐磨、耐热衬板、炉内滑块、滚轮、扭转辊、夹送辊、轨梁轿正圈、锯钻床备件、中厚板、热轧板刀片、纵剪圆盘剪刀片、等高难度机械备件。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。