内切

The cell lysate was used for Western blotting to examine the expression of the target protein.

结果 PCR、酶切和DNA测序结果均表明重组质粒pcDNA3-flag/HPC2构建正确，并可在HEK293细胞内高效表达。

Cut the fish fillet into the small chunks, marinate with salt for 25 minutes.

将鱼肉改切成小块，放入碗内，加盐腌上25分钟

Methods: A bonygroove was made on the upper rim of the acetabulum, and extended medially and upward on the ilium.

切取带有髂骨内板骨膜和软骨的全层骨块，嵌入骨槽中，形成杯口状，骨膜伸展贴附在髂骨外板上。

Based on this background, maxillary central incisors was prepared as residual roots model, the materials which has the similar physical properties and capabilities as that of the alveolar bone and periodontal membrane respectively were chosen.

基于上述情况，本实验以人离体上中切牙制备实验模型，以力学性能近似的材料模拟牙槽骨、牙周膜和桩冠修复后的上前牙，制作与上前牙口内受力角度相同的夹具及压力头，形成加载测试系统。

The voltage fluctuation and the harmonic distortion are limited within the demand of the national standard with the thyristor controlled reactor/transformer, multigroup switchable capacitor banks and hybrid active filter in the 10KV grid.

在10KV侧采用分组投切的滤波器，晶闸管控制的电抗器/变压器及混合滤波技术，使电网的电压波动及谐波电压因素限制在国家标准的范围内。

In this paper an attempt is first made to apply the above-mentioned three geothermometers in mylonite by using syn-tectonic neogenic muscovite, biotite and chlorite of mylonites in a ductile shear zone in the southern segment of the Tanlu fault zone.

本文利用郯庐断裂带南段韧性剪切带内糜棱岩中同构造新生的白云母，黑云母和绿泥石，首次尝试上述3种地质温度计在糜棱岩中的应用。

Chievo have some important players out, but they have assimilated Del Neri's ideas well and they are in good form.

切沃有些很重要的球员不能上场，不过他们会很好的听从主教练内利的意见，他们目前处于很好的状态中。

The Ca level was also significantly decreased after the operations but it only had statistic significances in group Ⅰ. Conclusion Both subtotal parathyroidectomy and total parathyroidectomy with parathyroid tissue transplantation in the forearm have therapeutic effects for renal secondary hyperparathyroidism and the effects had no significant differences in the short term.

对内科治疗无效的肾性继发性甲状旁腺功能亢进患者，甲状旁腺次全切除术及甲状旁腺全切＋前臂移植术都是有效的治疗方法，且两种手术方式在术后短期内（术后1天及6个月）的治疗效果无统计学差异。

The effect of division platen superheater arrangement on velocity deviation has been studied for the first time.

通过对炉内流场的详细测量，研究了切向燃烧锅炉水平烟道烟速偏差的形成机理。

Platina puts Martino's "Libro" in regional context, writing about ingredients coming from the regions, including perch from Lake Maggiore, sardines from Lake Garda, grayling from Adda, hens from Padua, olives from Bologna and Piceno, turbot from Ravenna, rudd from Lake Trasimeno, carrots from Viterbo, bass from Tiber, roviglioni and shad from Lake Albano, snails from Rieti, figs from Tuscolo, grapes from Narni, oil from Cassino, oranges from Naples and moray from Campania.

普拉提纳了马蒂诺的"书"的区域范围内，写成分来自各地区，包括来自马焦雷湖鲈鱼，沙丁鱼从加尔达湖，格雷从阿达，母鸡从帕多瓦，橄榄从博洛尼亚和皮切诺，大菱鲆从拉文纳，陆克文湖Trasimeno ，胡萝卜从维泰博，低音从伯， roviglioni和鲥鱼阿尔巴诺湖，蜗牛从瑞耶提，从Tuscolo无花果，葡萄从Narni ，石油从蒙特卡西诺，橙子从那不勒斯和鳝由坎帕尼亚。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新