内切

An enzyme that promotes the hydrolysis of urea to form ammonium carbonate.

核酸酶水解核酸的酶，如核酸内切酶和核酸外切酶

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

The gene CD18 segment was amplified by reverse transcriptase PCR including the mutation site 383 of CD18 mRNA coding sequence, and the PCR product was digested by TaqⅠrestriction endonuclease, which was regarded as the diagnosed technology of BLAD.

用反转录-聚合酶链反应方法扩增含有383位碱基突变的CD18基因片段，然后对扩增产物用限制性内切酶TaqⅠ进行酶切处理，根据电泳后酶切片段来进行诊断。

In the afternoon delivers diploma's picture in the teacher in charge hand, the impresario if a love smiles the old woman, a little corrupt Qian Dan is very lovable, it is a pity the high school entire grade has not taken the group photograph on actually my one, is very regrettable, although does not have the human who is reluctant to part with very much, but keeps a memento well also, what a pity does not have my face, but like this also good, changed attractive they have not also had the evidence saying that the NONO high school was a circle, inscribes circle which Canada circumscribed ha.

下午把毕业证书的照片送到班主任手里，班主任是个爱笑的老女人，有点贪钱但还是很可爱的，遗憾的是高中整年级就却我一个没拍集体照，很遗憾啊，虽然没有很留恋的人，但留个纪念也不错的嘛，可惜没有我的脸，不过这样也好，变漂亮了他们也没证据说NONO高中是个圆，内切加外切的圆哈哈。

In this model, the enzyme system includes the three components of the endo- and exo-glucanase, as well as β-glucosidase.

模型中纤维素降解酶系统包括纤维素内切酶、外切酶和β-葡萄糖苷酶三个组分。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

Mitochondria1 controlregion and cytochrome b gene of Bronze gudgeon in the lower reaches of the Yangtze River were cloned by PCR . Cloned fragments were digested by ten kinds of restriction endonucleases .

对长江下游铜鱼线粒体DNA 控制区和细胞色素b基因进行PCR扩增，扩增片段用10种限制性内切酶酶切。

Methods: The human MDK cDNA was amplied from the BGC823 cell line by high fidelity PCR.

扩增人MDK编码序列基因片段，经限制性内切酶酶切后将目的片段插入pLXSN逆转录病毒载体。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。