内切

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

At a constant shearing rate,the influences of montmorilonite loading on the shearing stress and apparent viscosity were similar.The reduction of the viscous activation energy with the increm...

PA611/蒙脱土纳米复合材料的粘流活化能随剪切应力的增大而降低，说明在恒定剪切应力下其可在较宽的温度范围内加工、成型。

Consumable mainly are: Italy Biesse high quality 0.38 and 0.55mm types; British Cheshire high quality ceramic anilox roller and chrome anilox rollers; Doctor blades from Swiss, Sweden, Germany and Janpan; Australia Rotometrics diecutting cylinders, magnetic cylinders, flexible die and hot foil cylinders; Recyl waterbased and UV general cleaning solution, Cobra anilox roller cleaning materials, steel brush from France ; Screeny from Swiss and US; High quality printing cylinders with harden gear; idle cylinders; cooling cylinders; ink rollers and so on.. Related equipment mainly are: Alphasonics ultrasonics cleaning machine; Non-stop splicer, rewinder and sheet delivery; Plate mounter;Offline Printing quality inspection machine with slitting faction; cold foil stamping; hot foil stamping unit with saver and hologram function; web gravure unit; GEW UV curing machine; spare parts and UV lamp.

耗材主要有：意大利BIESSE比艾斯高质量双面胶；英国CHESHIRE高质量网纹辊；瑞士，德国和日本刮墨刀；澳大利亚ROTOMETRICS模切辊，磁性辊，磁性刀皮和烫金辊；法国RECYL水性，UV通用清洗液，COBRA网纹辊清洗膏，钢丝刷；进口丝网；英国超声波清洗机用浓缩清洗液；高精度印版辊带有精磨齿轮，导纸辊，水冷辊；配套设备主要有：英国ALPHASONICS超声波网纹辊清洗机；不停机放卷，不停机收卷和模内标收集台；装版机；分切复卷和质量检测设备；冷烫金装置，带有印刷定位烫功能的热烫金装置；凹印单元； GEW UV固化设备，零配件，UV灯管。

Consumable mainly are: Italy Biesse high quality 0.38 and 0.55mm types; British Cheshire high quality ceramic anilox roller and chrome anilox rollers; Doctor blades from Swiss, Sweden, Germany and Janpan; Australia Rotometrics diecutting cylinders, magnetic cylinders, flexible die and hot foil cylinders; Recyl waterbased and UV general cleaning solution, Cobra anilox roller cleaning materials, steel brush from France ; Screeny from Swiss and US; High quality printing cylinders with harden gear; idle cylinders; cooling cylinders; ink rollers and so on..

耗材主要有：意大利BIESSE比艾斯高质量双面胶；英国CHESHIRE高质量网纹辊；瑞士，德国和日本刮墨刀；澳大利亚ROTOMETRICS模切辊，磁性辊，磁性刀皮和烫金辊；法国RECYL水性，UV通用清洗液，COBRA网纹辊清洗膏，钢丝刷；进口丝网；超声波清洗机专用浓缩清洗液，印版辊，导纸辊，水冷辊；配套设备主要有：超声波网纹辊清洗机；不停机放卷，不停机收卷和模内标收集台；分切复卷和质量检测设备；冷烫金装置，带有印刷定位烫功能的热烫金装置；凹印单元； GEW UV固化设备，零配件，UV灯管。

Methods One hundred and forty patients were divided randomly into two groups and underwent open lateral sphincterotomy or midline spincterotomy.

采用随机对照临床观察方法，比较140例患者采用侧位内括约肌切扩缝合术和后正中切扩术的疗效、并发症及愈合时间。

We have got the following conclusions from the simulation in the thesis:(1) In even diameter hole, the tangental velocity has the characteristic of combination vortex; with the boundary of points for maximal speed, the insides is norm forced vortex, the outside is norm free vortex; the axis symmetry of tangental velocity is very well.

等直径工件孔内的螺旋流场，其切向速度分布具有组合涡的特点，以最大切向速度点形成的面为界，内部是强制涡，外部是自由涡，其轴对称性很好。

While with the Coriolis force omitted, the flow in the cavity is under the influence of centrifugal buoyancy force, which can develop from stable to unstable as this force enhances. The centrifugal buoyancy force could lead to flow unstably.

哥氏力垂直于〓与〓构成的平面，对径向速度导致切向作用力，对切向速度导致径向作用力，在该力的作用下盘腔内轴向通流诱导的旋涡被压扁，尽管涡强有所增加，但是盘面的平均换热基本减弱。

Hot dogs in 10 minutes 美男子10分钟吞68热狗 Joey Chestnut, 25, Saturday chomped down a record 68 hot dogs in 10 minutes, capturing his 3rd straight annual July 4 hot-dog eating contest at Coney Island, New York, CNN reported.

25岁的美国男子乔伊·切斯纳特打破了吃热狗比赛的世界纪录。据CNN报道，7月4日在纽约康尼岛举办的年度吃热狗大赛中，切斯纳特在10分钟内吃掉68个热狗，这是他连续第三次夺冠。

Cysts form anywhere in the incisive canal located in the palatine bone and behind the alveolar process of the maxillary central incisors, or in the palatal soft tissues that overlies the foramen, called the cyst of the incisive papilla.

无论是在切牙管任何位置的囊肿都位于上颌骨，上颌骨中切牙牙槽突的后方，或者在覆盖于壳顶孔上方的软组织内，这种又被称为龈乳头囊肿

The dish washer includes one washer body with inside washing tank, rotary door in the front of the washer body, and one sealing latch set between the washer body and the door to raise the sealing property of the door. The sealing latch includes one hook unit set inside the hole on the washer body side, one sealing unit exposed outside the washer body and contacting closely to one side of the door in close state, one central slot for telescoping the hook unit, and one expansion joint in the joint part to avoid concentration of stress.

本发明包括洗碗机内部具备了洗涤槽的本体，设置在本体的前面转动的门，设置在本体前面和门之间，当关闭门时提高门和本体前面的封闭性的碰销；其中碰销包括：在本体前面一侧形成的插入孔中设置的挂钩部，露出于本体外部，在门关闭时，紧贴门一侧面的密封部，为了使挂钩在插入孔内直径方向伸缩，而在其中央部位形成的切缝，其特征在于密封部上形成了与切缝相通，防止挂钩伸缩时应力集中到挂钩部和密封部之间结合部位上的伸缩槽。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新