内切

This paper validate the good or the bad of reachability from three point of view: firstly, putti

针对可达性指标，本文从三个角度进行验证：第一，提出可达时间的概念，给出可达时间的求解方法，从定量的角度来衡量定性指标；第二，从视觉可达的角度来评价可达性的好坏，提出视觉可达性的验证方法：相对位置验证法和视野内切锥验证法，并给出计算机辅助验证流程；第三，从作业空间可达性的角度来评

Finally, all mutations of FV gene in probands and their family numbers were confirmed by .restriction enzyme analysis. Their occurence were investigated in the control group.

用限制性内切酶证实变的FV基因在患者家系成员中的分布情况，并在正常对照人群中进行了多态性分析。

This variation may be a genetic factor for obese subjects in China to develop type 2 diabetes mellitus.

近年来报道表明，位于3′非转录区上的内切酶SacII的限制性片段长度多态性与

These data suggest that the terminal hairpin structure and ITRs of PfDNV play a key role in the rescue and gene correction process: sequence at the junction of the terminal palindrome of PfDNV genome and the pUC119 can form typical Holliday structure, it can be cutted by the special endonuclease, then the exposed 3'hairpin structure serving for a self-primer to initiate viral DNA replication; and the intact extremity acting as a template to repair another altered end within inverted terminal repeats.

说明存在着一种拯救和基因修复机制，推测与PfDNV基因组末端结构（Hairpin、ITR）有密切关系：PfDNV基因组与pUC119质粒连接处可形成Holliday结构而被识别该结构的核酸内切酶所切割，游离下的基因组3'发夹结构作为引物起始复制，然后被置换链的完整的5'ITR作为模板修复部分缺失的另一端ITR，推测这个过程需要病毒NS蛋白以及细胞因子的参与。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave.

NSC-7在14 d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸； NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30 min后，仍能够保持分解能力， 105℃处理30 min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid.

NSC-7在14d的培养过程中，使稻秆分解73.6%；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6％of rice straw within 14d. 10 material were detected in volatile products by gas chromatography mass spectrometry,and it was mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80 and freeze℃-drying for four years. It could remain its degrading ability after 90 of high ℃temperature treating for 30min, and resume its degrading ability after subcultured 2 times after 105 ℃of high temperature treating for 30min in autoclave.

NSC-7在14d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4a后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

Various microarray-based methods depend on different pretreatments including immunoprecipitation and restriction digestion.

各种芯片技术以不同的DNA预处理方法为基础，包括免疫沉淀和限制性内切酶等。

The effect of anti-oxidation of hydrolysate hydrolyzed by Alcalase is close to that of Vc, BHA and BHT.

经过比较，碱性内切蛋白酶水解物的抗氧化性与抗氧化剂Vc、BHT、BHA的抗氧化效果接近。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新