内切

Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.

采用PCR技术和限制性内切酶片段长度多态性的方法，检测中国北方汉族168个核心家系的基因型。

Methods A PCR-based RFLP procedure was employed to detect the alleles and genotypes of PPARγ gene in 111family trios including 333 subjects of Han descent population in the north of China.

采用PCR技术和限制性内切酶片段长度多态性的方法，检测中国北方汉族111个核心家系，共333个研究对象的PPARγ基因的等位基因和基因型。

Finer surface finishes also may be needed to eliminate unnecessary stress concentrations, especially in areas of machined radii or undercuts.

起光滑表面作用的涂层还应当避免不必要的应力集中，尤其是机加工倒角或内切槽。

Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study.

此外，本研究对该方法的关键步骤，包括限制性内切酶的选择、接头与引物设计等，进行了优化。

Methods A method combining pulsed field gel electrophoresis with low-frequency-cleavage restriction endonucleases was established to analysis DNA of Enterobacter cloacae.

方法利用脉冲场凝胶电泳结合低频限制性内切酶技术对阴沟肠杆菌染色体DNA进行分析。

You can play with two central mid fielders, two wingers and two strikers and still create a third attacking line with movement of other people so if a winger comes inside he can work in between lines and the full back goes forward.

你可以让两名中场、两名边锋和两名前锋一起上场，而且你还可以通过其他队员的跑动创造出第三条的进攻线。所以如果一名边锋内切的话，他可以在两条线之间进攻，同时后卫线也可以前移。

You can play with two central mid fielders, two wingers and two strikers and still create a third attacking line with movement of other people so if a winger comes inside he can work in between lines and the full back goes forward.

当你使用两个中前卫，两个边路和两名射手时，通过其他球员的运动一样可以创造出第三条攻击线。如果一个边锋内切，那么他将会在两条线之间活动并吸引后卫向前移动。

Cervical spine digital models (C3~C7) were reconstructed, and obtained the biggest projection borderline and its inner circle of C3~C7. Then recorded the radius of the inner circle of each inner borderline and its center coordinate point. Simulating fixation screw were entered C3~C7 in different angles of transverse plane direction.

三维重建得到C(下标 3-7)数字模型，从不同方向观察各椎骨形态正常；得到C(下标 3-7)在不同外偏角方向椎弓根通道点云投影及其内边界、内切圈；模拟植入C(下标 3-7)，各个节段不同外偏角方向最大螺钉。

objectiveto explore a newsimple and effective surgery technique of eyebow lackness.methodstake aitem ofintegrity scalp with hair from the rest head or the back of the auris.separate the hair trunk and bursa with special appliance.

目的：寻求一种治疗眉毛缺损的简单有效的手术方法方法：在耳后或枕后发际内切取一条带毛发的完整头皮。利用特殊器械将毛干及毛囊完整分离，用植毛针把带完整毛囊的毛发移植于眉毛缺损处。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新