内切

Zymologic analysis showed that the molecular weight of this β-mannanase was approximately 47 kD. The enzyme was stable when pH ranged from 5.0 to 6.5 and could be activated by Na+ and Ba2+. With an optimal temperature of 50°C. Action mode analysis of TLC re-vealed that the enzyme belonged to the endo-β-mannanase family.

酶学性质分析：该酶分子量约为47 kD，酶的最适反应温度为50°C，在pH 5.0~6.5之间该酶的稳定性较好； Na+和Ba2+对该酶有激活作用，用TLC对酶产物分析，表明该酶为内切β-甘露聚糖酶。

The coden selection of the designed Cecropin AD gene was carried out according to the coden usage of yeast. The designed gene is 140 bp in length.

为适应酵母表达需要，基因中编码氨基酸残基的密码子以酵母偏爱密码为基础，借助DNASIS软件进行适当调整以消除不合适的密码子串联组织、潜在的二级结构和不适当的限制性内切酶位点。

He is particularly effective when playing on the right and bamboozling defenders by cutting inside onto his left foot.

他在右路活动时显得格外有效率，因为他可以选择内切，将球交至左脚迷惑对手。

Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete gene of α subunit and β subunit was obtained by inverse PCR.

以环化的限制性内切酶消化的基因组片段为模板，用反向PCR的方法扩增、克隆并测定了α亚基和β亚基的完整基因序列。

The enzyme activity was determined according to the velocity of the formation of halos on the plates.

建立了双层平板筛选由定向进化方法产生的瑞氏木霉内切葡聚糖酶Ⅲ突变体文库的方法，根据水解圈在平板上形成的速度判断酶活高低。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

应用不同的限制性内切酶消化玉米基因组DNA，以〓p标记的mF2KP cDNA片段作为探针进行Southern杂交分析。

This probe hybridizes to a PstⅠ restriction fragment of 3. 2kb in intact chromatin.

其次用DNaseⅠ处理Jurkat细胞核后，纯化基因组DNA，经PstⅠ限制性内切酶酶解并作Sothern blot分析。

Right backs all across the country were given a torrid time as Overmars ran at them and jinked inside or sprinted onto weighted passes inside the full back from the likes of Petit and Bergkamp.

整个英国的右后卫都受到了奥维马斯的折磨，他带球过人，急转内切，或者用速度硬吃对方，接到佩蒂特或者博格坎普妙传直杀后卫身后。

RESULTS: The coding sequence of a new gene and its spliced variant were cloned and identified successfully.

结果 构建的表达载体经过限制性内切酶分析和DNA序列测定，证实准确无误。

Bioinformatics analysis showed that over 150 AP2 genes in Arabidopsis could be divided into five subfamilies on the basis of number of repetition and sequence of AP2 domain.

AP2基因曾被认为植物所特有，但最近在蓝藻、线虫和病毒中发现了具有AP2结构域和位点特异核酸内切酶的蛋白。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新