内切

Phasianus ; Chrysolopus ; Mitochondrial DNA ; Restriction map ; Molecular phylogenetic tree

雉鸡；锦鸡属；线粒休DNA ；限制性内切酶图谱；分子系统树

86 hypertensive patients with ischemic stroke . 85 hypertensive patients without ischemic stroke and 90 healthy blood donors were randomly enrolled in this trial . p-fibrinogen gene -455G/A polymorphy was analyzed from all subjects by polymerase chain reaction with restrictive enzyme HaeIII(PCR-RFLP method).

随机抽取住院高血压脑梗死病人86例，高血压非脑梗死病人85例，健康老年人90例，β-纤维蛋白原基因启动子区域-455G／A的多态性分析用多聚酶链反应加HaeⅢ内切酶检测。

However, research on bacterial digestive enzymes identified a prolyl endopeptidase that can break the chain into innocuous components.

但是，对名为脯氨基内切脢的细菌性消化脢进行研究发现，其能将链分解成无毒成分。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

设计合成一对特异引物，分别带有 SnaBⅠ和 NotⅠ的限制性内切酶的识别位点，以 pPIC9K-NGF重组质粒为模板，用 PCR 方法扩增 NGF cDNA 片段。

It could be included that the PGase from the strain NT 33-6 is a thermostable, alkaline and endo-type enzyme.

据此可以得出结论，嗜碱芽孢杆菌突变株NT 33-6产生的PGase是一种热稳定碱性内切酶。

Therefore,a sequence specific artificial zinc finger proteinis created when replacing the amino acids in the specific sites of C2H2 zinc finger protein and then fusing the protein to other activation or repression domains,while remaining the backbone of the zinc finger protein unchange...

ZFP可以介导靶基因的转录调控，抑制或激活特定基因的表达与配体依赖的靶基因激活或抑制；对DNA进行修饰，如人造限制性内切酶，重组酶，整合酶；抗病毒感染等。因此，人造锌指蛋白应用前景广阔，研究价值显著，是未来人类基因治疗的革命性的工具。

The unmethylated probe can be cut in the recognition site by restriction endonuclease, causing the restoration of the fluorescence of TAMRA.

限制性内切酶可切割未发生甲基化修饰的探针，导致探针的发夹结构遭到破坏，引起TAMRA荧光信号的恢复。

Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele or by conditioning on genotype with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups.

采用聚合酶链反应-限制性内切酶片段长度多态性方法检测T2DM患者（病例组121例）和健康对照（对照组142例）3个cPLA2基因（PLA2G4A、PLA2G4B和PLA2G4C基因）的基因型；借助UNPHASED分析平台，利用等位基因条件分析和基因型条件分析方法分析基因的联合作用与T2DM的遗传相关性。

A strain KX6, producing thermotolerant endoglucanase, was isolated from compost. The morpholo-gical identification and 16S rRNA sequence analysis showed it belongs to Streptomyces xylophagus.

从堆肥中筛选到一株产耐热内切葡聚糖酶的放线菌菌株，通过形态观察和16S rRNA序列分析，鉴定为蚀木链霉菌。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究，碳源为1%羧甲基纤维素钠，氮源为1%豆粕粉，250ml三角瓶30 %装液量，接种量为2%，培养基初始pH为8.0，培养温度为40℃，200r/min培养48h后，发酵液中内切葡聚糖酶活达到0.538IU/ml。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新