内切

In order to clone AVR-Pia, the flanking sequence of MS220K4 was used as a probe to screening the TAC library and 17 positive clones were obtained. Further restriction enzyme mapping and Southern blot analysis on 10 of the 17 positives clones narrowed the avirulence gene to a region of 10 kb.

为克隆AVR-Pia基因，以MS220K4的侧端序列为探针筛选稻瘟菌基因组TAC文库，得到17个阳性克隆，通过对其中10个进行的限制性内切酶分析并结合Southern杂交确定了目的基因所在区域。

The results showed that the microbial community could degrade 39.6% of rice straw gross weight within five days. When the culture medium volume was 1/5 of the whole and pH at 6 on the 5 days culture, the CMC reached the highest of 14 IUml^(-1). During the rice straw degradation, more than ten kinds of products were detected using GCMS. DGGE detected the dynamic change of microbial community composition, and the microbial composition changed greatly in different periods. The phylogenetic tree derived from 16s rDNA sequence was found that the closest relatives belong to Clostridium sp., Brevibacillus sp., Bartonella sp.、Bacteroidetes sp.

5d后稻秆总减重量达到39.6%，在培养基占总体积1/5、pH=6、培养第5天时纤维素内切酶活性表现最高，达到14IUml^(-1)；培养过程中发酵液中有10余种挥发性产物，且不同时期产物的种类和浓度变化很大，从DGGE图谱发现在培养不同时期菌种组成有很大差异，通过各条带近缘种16s rDNA扩增信息构建系统树可见，各条带近缘种分别归属Clostridium、Brevibacillus、Bartonella、Bacteroidetes4个属。

Methods We used the automatic DNA sequencing machine (Model 377) to detect the nucleotide sequence of the inserted part of the recombinant plasmid pBX1 from Borrelia burgdorferi B31 strain.

方法采用377型DNA自动测序仪对莱姆病螺旋体重组质粒pBX1的插入片段进行DNA序列测定，并通过计算机软件对其进行限制性内切酶酶谱分析。

Methods We cut a piece of complete scalp from pulvinar or after the ear, and used a special apparatus separate the hair shaft and follicle, use the hair plantation needle plant the scalp on the place which have the eyelash coloboma.

在耳后或枕后发际内切取一条带毛发的完整头皮。利用特殊器械将毛干及毛囊完整分离，用植毛针把带完整毛囊的毛发移植于睫毛缺损处。

MethodsWe cut a piece of complete scalp from pulvinar or after the ear, and used a special apparatus separate the hair shaft and follicle, use the hair plantation needle plant the scalp on the place which have the eyelash coloboma.

在耳后或枕后发际内切取一条带毛发的完整头皮。利用特殊器械将毛干及毛囊完整分离，用植毛针把带完整毛囊的毛发移植于睫毛缺损处。

objectiveto find an effective and simple operation method to treat the eyelashes coloboma.methodswe cut a piece of complete scalp from pulvinar or after the ear, and used a special apparatus separate the hair shaft and follicle, use the hair plantation needle plant the scalp on the place which have the eyelash coloboma.

目的：寻求一种治疗睫毛缺损的简单有效的手术方法。方法：在耳后或枕后发际内切取一条带毛发的完整头皮。利用特殊器械将毛干及毛囊完整分离，用植毛针把带完整毛囊的毛发移植于睫毛缺损处。

A cDNA for endo-β-1,4-glucanase was isolated by RT-PCR, and rapid amplification of cDNA ends reaction from taro leaves (Colocasia esculenta var. esculenta).

本研究利用RT-PCR及rapid amplification of cDNA ends的方法，自槟榔心芋叶片选殖内切型纤维素分解酵素cDNA。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

In this research, we investigated the edaphon community structure from Gaofeng forest-farm Eucalyptus plantation in Guangxi Nanning by PCR-RFLP technique and 16S rDNA sequence analysis.

本研究利用限制性内切酶片段长度多态性和16SrDNA序列分析相结合的方法研究了广西南宁高峰林场人工桉树林中土壤微生物的群落结构。

Objective To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis and random amplified polymorphic DNA analysis for molecular typing of Neiss eria gon orrhoeae isolates.

目的 评价脉冲场凝胶电泳限制性内切酶分析及随机扩增多态性DNA分析对淋球菌分离株进行基因分型的能力。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新