内切

However, CDH has little synergism with cellobiohydrolase or endoglucanase in cellulose degradation. But, if the substrate was pretreated with CDH in the presence of cellobiose, the rate of the hydrolysis of the substrate by cellobiohydrolase or endoglucanase increased.

CDH与纯化的外切纤维素酶或内切纤维素酶或内切纤维素酶之间没有协同作用关系，但在纤维二糖存在条件下底物事先用CDH处理后再用上述纯化的酶水解，作用明显增强。

And obtained recombinant plasmid PGEM-VP3 by linking prokaryotic expressionby vecto r PGEM-4T-1,and identified by PCR、double restriction enzyme analysis and sequence analysis,the result showed that it's true.

用EcoR I和XhoⅠ双酶切该片段并回收该片段，将此基因片段克隆至相同酶切回收后的PGEM-4T-1原核表达载体中，获得重组质粒PGEM-VP3，经PCR鉴定，限制性内切酶双酶切分析，证实了重组质粒的正确性。

Total 122 entophytic bacterial strains were categorized preliminarily to 35 groups based on colony morphology. 16S rDNAs were amplified with universal primers of bacteria, all the strains could produce a single band about 1500bp. The PCR products were digested with restriction endoenzyme HaeⅢand Msp Ⅰrespectively, and the different patterns were obtained for each enzyme, by combining all the restriction patterns for the two enzymes, the 122 strains could be grouped into 39 16S rDNA genotypes. The results indicated that endophytic bacteria from oilseed rape were genetically diversity.

分离获得的122株内生细菌，根据菌落形态可以划分为35类；利用细菌通用引物对16S核糖体DNA进行扩增，获得约1.5 kb片段，分别用内切酶HaeⅢ和MspⅠ对扩增产物进行限制性酶切，产生不同的酶切图谱；根据酶切图谱聚类分析结果，所有供试菌株被归为39类，这一结果从遗传上显示油菜内生细菌类群的多样性。

The formation of silique dehsicence susceptibility was investigated in terms of water content, synthetic rate of ethylene, enzyme activity of exo polygalacturonase and end cellulase , cell alteration in dehiscence zone in silique and silique dehiscence susceptibility during silique development.

从油菜角果发育过程中角果含水量、乙烯合成速率、外切 PG 酶和内切纤维素酶活性，以及角果裂区切片观察等方面研究了角果开裂敏感性形成的原因⒚花后50d 的角果果柄端壳状果瓣和线状果瓣间的裂区薄壁细胞膨大，在高倍光学显微镜下可见胞间空隙开始形成⒚此前6d 内乙烯合成速率迅速上升并出现第二次合成高峰，与之同步发生外切 PG酶和内切纤维素酶活性急剧上升，而乙烯合成第二高峰出现于角果含水量低于80％以下⒚由此看来，角果失水引起乙烯合成第二高峰出现，从而导致水解酶活性上升，最终导致果瓣裂区薄壁细胞膨大，分离和胞间空隙形成及角果开裂敏感性产生

749Mg/ml and 0.778u/ml, respectively. 5. Study on the viscosity changes of reaction solution and analysis of enzymatic hydrolysis products by TLC, HPLC showed that ChiB was an endo-type cleavage enzyme. It decreased viscosity of reaction solution, hydrolyzed β-(1, 4) glucosidic bond of chitosan, acted on GlcN-GlcN, GlcN-GlcNAc and/or GlcNAc-GlcN glucosidic bond. It did not act on GlcNAc-GlcNAc glucosidic bond. Hydrolysis products of chitosan were composed of chitotriose, chitotetraose and chitopentose.

通过测定酶解液体系粘度和对酶解产物的TLC、HPLC分析，在国内首次深入地研究并报道了内切、外切壳聚糖酶的酶解作用模式，结果表明：酶ChiB是一种内切酶，以内切方式水解壳聚糖的β-（1，4）糖苷键，即作用于GlcN-GlcN、GlcN-GlcNAc和／或GlcNAc-GlcN糖苷键，不能作用于GlcNAc-GlcNAc糖苷键；酶解终产物以壳三糖、壳四糖、壳五糖为主。

The CORNELL/COPERSUCAR/USPA project for the application of RELP technology in sugarcane encompasses three objectives: aEstablishing measures of genetic distance among and within species of the Saccharum complex and hybrids. bDevelopment of a genetic linkage map applicable to the saccharum genera.

CORNELL/COPERSUCAR/USPA 合作的多倍体的核酸内切酶内切限制断段变化技术应用于甘蔗，包括三个目的：a建立甘蔗属的合成物和杂种种内和种间的基因距离的测定。b发展应用于甘蔗属的基因链锁图。c努力建立分子标记和农学特性之间的链锁。

PCR productsdigested by SnaB Ⅰ and Not Ⅰ were inserted into the correspondingrestriction site in the expression plasmid pPIC9K.

用限制性内切酶 SnaBⅠ和 NotⅠ酶切 NGF cDNA 的 PCR 产物，将其插入到载体 pPIC9K 中相应的限制性内切酶位点，构建表达载体pPIC9K-NGF.3。

All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.

利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。

MCP could effectively delay the softening of climacteric fruit, such as Starking and Fuji apples and Feicheng peach, but as different fruits had different softening mechanism, the mechanism of 1-MCP inhibiting different fruit softening was different: in Starking apples, the key enzyme to the softening is endo-and exo-PG, while during Feicheng peach fruit softening, the key enzyme was EGase before the 20th d and endo-PG after 20th d during storage at 0℃. 1-MCP inhibited fruit softening mainly by inhibiting PG activity in Starking apple while in Feicheng peach, by inhibiting the activity of EGase in the earlier storage time and endo-PG in the latter time. It was also found that LOX accelerated the initiation of the softening and was regulated by ethylene and 1-MCP in apple and peach.

2.1-MCP能较好的延缓红星、富士和肥城桃等跃变型果实硬度的下降，但是不同品种/树种的果实软化机理不同，1-MCP抑制其软化的机理也不一致：在红星果实中，对软化起主要作用的酶一直为内切和外切两种PG，而在肥城桃软化过程中，前20d以纤维素酶活性起主要作用，20d后软化以内切PG为主。1-MCP主要通过抑制红星果实中PG酶活性来抑制果实硬度的下降；在肥城桃果实中，1-MCP通过抑制前期纤维素酶和后期内切PG活性，延缓了果实硬度的下降。

First, according to the parts of materials processing operations, cutting tools and other relevant factors and choose the outline of the handle and part characteristics identify nine tools were cylindrical rough turning, cylindrical precision turning, external grooving cutter , internal thread cutter, Boring tool within the inner notch cutter, 93 ° left side knife, 93 ° right-side knife.

第一，根据本零件材料的加工工序、切削用量以及其他相关因素选用刀具及刀柄和零件的轮廓特点确定需要9把刀具分别为外圆粗车刀、外圆精车刀、外切槽刀、内螺纹刀、内镗孔刀、内切槽刀、93°左偏刀、93°右偏刀。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。