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The phenolic acid content of litter was significantly reduced with the decomposition time prolonging, and the releasing quantity of phenolic acid was the highest at the tenth day. The amounts of soil Aerobic azotobacter, Ammonifier and Aerobic cellulose-decomposer and the soil chemical properties such as pH, phenolic acid content, NH+4-N and available phosphorus were significantly affected by the litter aqueous extract of four decomposition stages.

凋落物分解的60 d中,凋落物残留量在前30 d内变化最大,30 d后无显著变化;凋落物中酚酸含量随分解时间的延长,呈显著降低的趋势,且在分解10 d时,酚酸释放量最大。4个分解动态的凋落物浸提液显著地改变了土壤好气性自生固氮菌、氨化细菌、好气性纤维素分解菌的数量和土壤pH值、酚酸含量、铵态氮、有效磷等化学性质。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状,由精子群、精荚基质及初级精荚壁和次级精荚壁组成,初级精荚壁与精荚基质是相连续的,它们之间无明显界限,两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成,但后者不含精子,后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物,可能与形成精子细胞核中的泡状带和膜状带有关,在交配后的雌虾,其头胸部腹面上粘着的精荚中,其基质内已不见絮状和同心圆泡,仅见稀疏的纤丝。

We successfully identified JSRV mRNA expression and proviruses DNA in sheep lung tissues infected with JSRV and control group has no postive signals,Validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.

用地高辛随机引物法标记exJSRV特异的env片段,制备探针,原位杂交检测SPA肺组织中的RNA及前病毒DNA,结果表明SPA患羊肺组织内有JSRVenv基因mRNA的表达,同时也检测到了前病毒DNA,而相应的阴性对照却无阳性信号,证实外源性病毒特异的DNA探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。

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