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If you would attain that Truth, if you would become perfect in the knowledge of that Truth, you must go through this process of renunciation, setting aside those things which have no value, putting aside your little knowledge in order to acquire greater knowledge, putting aside your little wisdom in order to acquire greater wisdom; and so when you reach the abode where there is no cloud of doubt, of misjudgement, where there is no question of perverting judgment, or of false thoughts, of false emotions, of fleeting affections, then you are truly in possession of the Truth, then truly are you like myself -the Beloved.

其他人也许鼓励,其他人也许阻挠,但是在只有在你才有去行走的力量,只有在你才有决心,只有在你才有智慧,如果你想获得那真理,如果你想在真理的知识方面变的完美,你必须走过这个弃绝的过程,把那些没有价值的事情放在一旁,把你的小知识放在一旁为了获得更伟大的知识,把你的小智慧放在一旁为了获得那更大的智慧;并且因此当你到达那住所,在那里没有怀疑的,错误判断的乌云,在那里没有滥用判断的,或者错误的想法的,错误的情感的,暂时的喜爱的问题的时候,那么你就真正地拥有了真理,那么你真正地像你自己——挚爱者。

There were three isozyme (AMY1, AMY2 and AMY3) at the locus of amylase in Qinghai Merino, moreover, there were two genotypes of AMY2A and AMY2O at the AMY2. Monomorphism was tested at the locus of AMY in Gansu Alpine Merino. The degree of genetic variability estimated by Nei's expected average heterozygosity for the 6 blood protein loci was relatively higher in Gansu Alpine Merino and in Qinghai Merino than that in others, which indicated that there are higher genetic variations and richer genetic diversity in Alpine Merino.

从6个血液蛋白位点的Nei氏预期平均基因杂合度所估计的群体的遗传变异以及与本研究其它群体的结果相比来看,甘肃高山细毛羊和青海细毛羊的群体的遗传变异相对较大,而其它群体的遗传变异则相对较小,说明高原型细毛羊群体遗传变异程度较大,遗传多样性较丰富,具有较大的选择潜力,为高原型细毛羊进一步选育提高提供了遗传基础。

Although Ms Roberts makes a valiant attempt to turn the sweep of Montenegrin history away from war, banditry and looting to discuss its contributions to literature and the theme of Montenegrin identity, she cannot get away from the fact that its history has been bloody in the extreme.

尽管罗伯茨女士在写作的时候极力想要避开门的哥罗历史上的战争,盗窃和抢劫,从它在文学上的的贡献以及有关门的哥罗身份的话题展开论述,但她不能回避的现实是----门的哥罗的历史实在是血腥至极。

Taking the Kubelka-Munk laws as foundation,the light interaction of ink and paper by existing models has been analyzed,including surface reflection of ink and multiple intemal reflection of paper,and light absorption and scattering and so on,the Clapper-Yule color prediction model has been modified aiming at the two possibilities of long and short distance of light scattering in newspaper.

以Kubelka-Munk定律为基础,研究了油墨、新闻纸在印刷中墨层的表面反射、承印物纸张的多重反射、墨层光的吸收和散射,针对其中的光散射距离的长短两种可能,修正了Clapper-Yule颜色预测模型,引入代表长短光散射距离的比例系数h,建立了新的颜色预测模型,计算得到h=0.43。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系,应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后,流式细胞仪技术检测CCR1和CCR2的表达,ELISA法检测细胞培养上清液白介素-8的浓度;体实验选用C57BL/6J小鼠,腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg),LPS刺激4 h后收集BALF和肺脏标本,计数BALF细胞总数,应用BCA法检测BALF蛋白浓度,Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜无节幼体期和无节幼体Ⅰ期在的中国对虾胚胎的间接免疫荧光检测,最早在肢芽期胚胎中检测到2A3阳性信号;在膜无节幼体期,2A3阳性血细胞平均直径约7μm,聚集成簇;在无节幼体Ⅰ期,血细胞形态更加完整清晰,平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜无节幼体期胚胎中出现,这些细胞同样成簇存在,在其细胞质外围可见点状荧光。

"One of the major problems with dialysis done in the traditional sense is that it tries to provide a lot of therapy in a short period of time, and it's difficult to clear toxins and fluid in that time," said Dr. Dylan Steer, a nephrologist with Scripps Memorial Hospital in La Jolla, Calif."Nocturnal dialysis provides a greater amount of toxin removal over a long period of time."

加州拉霍亚Scripps Memorial医院的肾脏病学家Dylan Steer博士说:"常规进行的透析治疗就是要在短时间进行大量的治疗,但其实在这么短的时间完全清除毒素和水分是很困难的,而夜间治疗则可以在较长的时间轻松就做到。"

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆再移向质膜外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆再移向质膜外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

The mathematical model and optimized result indicate that the magnetic field in the inner wall of the detecting coils is of non homogeneity. At a ratio R/L .2, the magnetic field along the axial length and within the range of 90% radial direction is nearly homogeneous.

数学模型和优化结果表明:在检测传感器线圈中部范围为非匀强磁场,且呈非线性变化,细长管磁场相对均匀;当选取 R/ L 。2 ,磁场在线圈轴向长度范围,径向在90 %范围可近似看作均匀磁场。

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