具菌托的

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。