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The constructed candidate for DNA vaccine was immunized in mice and tree shrew and it's anti-HBc IgG was detected.

以此为候选疫苗分别免疫小鼠和树,并检测其抗HBc IgG。

Its tip flicked twice around the tumbleweed, jerked it off the ground and back to the feed opening in the imitation tree stump within which the rest of the slurp was concealed.

舌尖绕着风滚草圈了两圈,然后猛得将其拉起来,往模仿树残桩里的进食口拖去,啜食者正是隐藏了里面。

With set2 paprameters, at √s=1.17 TeV the tree level and EW corrected sections of γγ-t2t2 increase by 81% and 78% respectively, compared with unpolarized tree level and EW corrected sections. Therefore, in some gaugino-like parameters, the effects of the photon polarization to squark pair production can improve the efficiency of detection at future linear colliders.

t2t2的set2参数条件下,在1.17 TeV处,树图截面和电弱修正的截面分别增加了81%和78%,因此在未来的直线对撞机上以γγ对撞模式产生标量夸克对时,在某些特定的类gaugino的参数条件下,考虑到光子的极化效应对其截面的影响将有助于提高对撞机的探测效率。

Seedling cultivation trials were carried out for the seeds of Malania oleifera from Ba-ma county in Guangxi province,with three different treatments,and their seedlings were transplanted in shaded and unshaded seedbed to observed their height and disease and pests.

对采自广西巴马县的不同母树的蒜头果种子按三种不同处理进行发芽试验,并将萌发后的幼苗移栽到露天苗床和遮荫苗床,观察其株高和病虫害情况。

In bathhouse of an open air, a flock of sturdy workers are bathing, watch on the tree that a few meddlesome monkeys mount bathhouse edge, bian Xiao looks by the side of the monkey only among them, look to feel comical more more, laugh finally so that drop to cultivate, roll about on the ground, other monkey base become aware questioningly, it comes to uprear, ask its place laugh why, that monkey still laughs ceaseless, path: Ha...

在一个露天澡堂里,一群强壮的工人在洗澡,几只好事的猴子爬上澡堂边的树上观看,其中一只猴子边看边笑,越看越觉得好笑,最终笑得掉下树来,在地上打滚,其他猴子基觉诧异,扶起它来,问其所笑为何,那猴仍笑个不停,道:哈哈。。。

Pyramidal lattice vector quantization has drawn extensive attention by its promising property recently. The coding method based on zerotree coding[1] has made a great coup during the last few years.

近年来,金字塔格型矢量量化方法由于其本身的特点和性质引起了人们的广泛兴趣,而文献[1]介绍的基于零树的编码也获得了极大的成功。

A new method for indexing large amounts of points inhigh-dimensional space is proposed.The basic principle is as follows:uniformly partition the data space by Lattice vector quantization,store the lattice points by Inverted File,organize the inverted file by Trie tree,and fast access the inverted file by Trie parallel search algorithm.

本文提出一种新的高维空间中点数据的索引方法,其基本原理是用格矢量量化均匀划分数据空间、用倒排文件存储格点、用Trie树实现倒排文件的组织和存储、用Trie并行搜索算法实现倒排文件的快速访问。

Far in the interior of the wood, where a noisy swarm of laborers were working, stood Waldemar Daa and his three daughters, and all were laughing at the wild cries of the birds, excepting one, the youngest, Anna Dorothea, who felt grieved to the heart; and when they made preparations to fell a tree that was almost dead, and on whose naked branches the black stork had built her nest, she saw the poor little things stretching out their necks, and she begged for mercy for them, with the tears in her eyes.

远在国内的木材,在嘈杂的群劳动者工作,站在瓦尔德马的DAA和他的三个女儿,和所有人都笑呼声野生鸟类,除一,最年轻的,安娜多萝西,谁觉得难过心脏;,当他们作好准备,下降了树几乎死亡,其赤裸裸的分支机构的黑鹳建造了她的巢,她看到了可怜的小东西伸展自己的脖子,她乞求宽恕他们,同泪水在她的眼睛。

The purification of black wattle tannin by gel column chromatography showed little effect on the complex stability constants.

用柱层析提纯的黑荆树单宁对其金属络合物的稳定常数的影响不大。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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