关节小鼠

The results show that: This tablet can decrease the rat"s footpad swell induced by Freud"s, inhibit the permeability of the blood capillary in inflammatory joint and decrease the 11-2 level in serum while increasing the rat"s body weight,(2) It can lower the rat"s temperature and the permeability of the blood capillary in inflammatory joint induced by collagen and inhibit the delayed type hypersensitivity,It can lower the stickiness of full blood and the accumulation of erythrocyte obviously inblood stasis model rats,lt can inhibit the formation of hemolysin and decrease the index of carbon granule clearance in mice, It can lower the permeability of celiac blood capillary and inhibit the granulomatous hyperplasia by sc cotton ,It can inhibit the pain induced by acetic acid and raise the pain threshold value of heat stimulation.

结果表明：关络通片能明显降低佐剂性关节炎大鼠左右足趾肿胀度，降低大鼠炎症关节的通透性，抑制其IL-2含量，对大鼠体重增长缓慢有一定的改善作用；能改善Ⅱ型胶原诱导性关节炎大鼠的表征指标及炎症关节的通透性，对其迟发型超敏反应有一定的抑制作用；对血瘀模型大鼠，能显著性降低其全血粘度、血浆粘度、红细胞压积；能抑制小鼠的溶血素抗体生成，抑制小鼠网状内皮系统的吞噬能力，具有一定的抑制机体免疫功能的作用，能抑制小鼠腹腔毛细血管通透性增高，抑制大鼠棉球肉芽组织增生，具有一定的抗炎作用；能抑制小鼠醋酸扭体反应、提高小鼠热板痛阈值，具有一定的镇痛作用。

Methods:Anti-inflammation of XSG was observed by mouse model of auricle swelling and rat model of ankle joint swelling caused by xylol and egg-white respectively,and analgesia was tested by hot-plate caused sole-ache and acetic caused bellyache.Gastric ulcer model rats was duplicated in by Reserpine and Acetate.

采用小鼠二甲苯性耳廓肿法、大鼠蛋清性关节肿法进行抗炎实验观察；采用小鼠醋酸扭体法、热板法进行镇痛实验观察；用利血平、腹腔注射及冰乙酸直接胃壁注射复制大鼠胃溃疡模型，观察香砂六君颗粒对两种胃溃疡动物模型的溃疡指数的影响并计算溃疡抑制率。

RESULTS:The SP and VIP content was evidently reduced in the treatment group compared to the model group; whole blood viscosity of blood rheology index, fibrinogen viscosity and packed cell volume were reduced in the treatment group as compared to the model group, which could evidently inhibit auricular swelling in mice and arthroncus in rats, raise the percentage of pain threshold, phagocytic index and rate of celiac macrophage and effect the spleen, the peripheral immune organ in mice.

结果：治疗组SP、VIP较模型组明显降低；血液流变学指标全血粘度、纤维蛋白粘度、红细胞压积测定，治疗组较模型组降低；能明显抑制小鼠耳廓肿胀和大鼠关节肿胀；提高小鼠痛阈百分率；提高小鼠腹腔巨噬细胞的吞噬指数和吞噬率；对外周免疫器官脾脏有明显影响。

METHODS The models of acute inflammation in ear of mice induced by dimethylbenzene,arthroncus in rats made by carrageenin,subacute inflammation of tampon granuloma in mice and adjuvant arthritis in rats were made,and all above animal models were treated with25,50,100mg/L of the bear bile lyophilized powder injection and observed the curative effects.

方法］ 分别制作二甲苯所致小鼠急性耳壳炎模型，交叉菜胶所致大鼠关节肿胀模型和小鼠棉球肉芽肿亚急性炎症模型。各组均分别给予25，50，100mg/L熊胆冻干针剂，观察疗效。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

Objective To identify the joint -specific expression profile of Borrelia burgdorfri in the murine hosts.

目的 建立伯氏疏螺旋体感染的小鼠模型，研究伯氏疏螺旋体在小鼠关节组织中的关节特异性基因表达谱。

These joint-specific genes might were involved in the survival and arthritis pathogenesis of Borrelia burgdorferi.

伯氏疏螺旋体在小鼠关节组织中存在独特的基因表达谱，这些在关节中特异表达的基因可能与莱姆关节炎的发生、发展有关。

Then the index of ulcer was measured and the ulcer inhibitory rate was measured.Results: The weight of ear of mice treated with XSG was reduced compared with that of normal group,and the joint swelling of rat was less than that of normal group. The latence of sole-ache caused by hot-plate and bellyache caused by acetic were both delayed and the times of skewing body within 15 minute were reduced compared with that of the normal group.

结果：香砂六君颗粒对小鼠二甲苯性耳廓肿有明显对抗作用；对大鼠蛋清性关节肿有一定对抗作用；对热板和醋酸所致小鼠疼痛香砂六君颗粒能明显延长其舔足潜伏期和减少扭体次数；对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒组的溃疡指数从模型组的46.26±1.64mm2和23.02±2.05mm2减少为34.75±0.82mm2和13.84±1.2mm2；对大鼠利血平型及乙酸型胃溃疡香砂六君颗粒的溃疡抑制率分别为51.43%和39.87%。

The results of flow cytometry in normal, B27 single transgenic mice and B27/hβ〓m double transgenic mice were 0.63％, 7. 87％ and 35. 87％ respectively. B27 antigen was also expressed in the cell surface and cytoplasm of colon, skin, testis and eyes of the double transgenic mice by the detection of immunohistochemistry, which was evident on the cell surface. 8 double transgenic mice bearing high copy (7 males, 1 female) developed spontaneous alopecia, psoriasiform dermatosis, joint swelling and nail changes in the rear paw.

免疫组化检测可见转基因小鼠的结肠、皮肤、睾丸和眼睛组织的细胞膜和细胞浆均有B27抗原的存在，尤以细胞膜明显。21只B27/h〓转基因阳性鼠中有8只出现脱毛和皮肤的银屑病样改变，同时伴有后肢的关节肿胀和趾甲变化。8只小鼠均为高拷贝，其中雄性7只，雌性1只。

Methods: The mice for experiment were immunizied with subcutaneous injection of type Ⅱ collagen to induce arthritis after ovariectomy. Severity of joint swelling, radioimmunoassay of serum estradiol (E2),osteocalcin and tumor necrosis factor, and pathological changes of joint, including changes on synovia, articular cartilage and bone, were observed once weekly.

切除小鼠卵巢后，Ⅱ型胶原皮下注射以诱导关节炎；每周1次观察小鼠的关节肿胀程度；组织切片光镜下观察小鼠滑膜、关节软骨及骨的改变；放射免疫法检测小鼠血清雌二醇、骨钙素、肿瘤坏死因子的变化。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。