关节刀

Objective:To treat the acute soft tissue injury of four limbs and around big joints, and evaluate it's effect by the change of hematome, swelling and ecchymosis, the functional recovery of limbs and the severity of pain before and after treatment.

目的：通过针刀对四肢肢体及较大关节周围急性软组织损伤的治疗，观察血肿、水肿及皮肤瘀血斑吸收情况，肢体功能恢复速度和疼痛的强度及持续时间，评估疗效。

Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损，骨刀切除软骨下骨到髓腔渗血为止(厚约4mm)，压迫后FG止血；取髂骨骨块，并尽可能保留松质骨，取下的骨块用PBS反复清洗，以除去血细胞，将松质骨填充在骨缺损处，松质骨面朝向关节腔，高度与周边软骨下骨齐平，把骨膜片生发层朝向关节腔，用无创伤缝合线缝合在周边的软骨或滑膜组织上；向EP管中加入1/2悬液体积的FG主体胶溶液并混匀，再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中；同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞；C组造成骨软骨缺损，FG覆盖创面后单纯用骨膜修复缺损。

Using acupotomology incise and separate the hip joint articular capsule and ligament and the periosteum on the collum ossis femoris and the soft tissue nearby the joint can cause slight hurt and haemorrhage. The hurt factor is release from the local tissue. Human self-rebuilding system is activated, Building bone function of osteoblast is accelerated, new bone is built quickly. New blood vessel and nerve ending is rebirth. Local tissue's blood supply and nerve nourishment is improved. The developing of bone necrosis is prevented. The necrotic part is re-blood vessel. The conditions of the necrotic's absorbing tissue and the new bone's replacing are established.

针刀治疗股骨头无菌性坏死是通过针刀对髋关节囊、关节囊韧带、股骨颈的骨膜和髋关节相邻周遍组织切割分离铲拨松解可以产生轻微创伤、出血、局部组织释放创伤因子，激发人体自我修复重建反应，激活成骨细胞造骨功能，加速新骨生成，促进新血管和神经终端组织再生与重建，使局部组织血流加速，改善血液供应和神经营养状况，阻止坏死进一步发展，使股骨头颈坏死区域再血管化，为坏死组织吸收和为新骨再生爬行替代过程创造条件。

The patients were divided into a needie-knife treatment group treated with needle-knife therapy at the upper and lower interspinal ligaments of the affected vertebral body and bilateral posterior joint capsules; and the acupuncture control group were treated with acupuncture at Laozhen, Ashi points and cervical Jiaji points, etc.

采用多中心临床随机对照试验方法，将患者分为针刀治疗组（在患椎上下棘间韧带和两侧后关节的关节囊处行针刀治疗）与针刺对照组（穴取落枕、阿是穴、颈夹脊等），在疗程结束时和治疗结束后6个月分别统计近、远期疗效。

The characteristic of the treatment by manipulation with acupotomy in tensive position is manipulating the adhesions and contractures of capsule in direction: including the zona of intertubercular sulcus, inferior-acromial and the decocted later part of the capsule, and with hydraulic distension and manipulation. Compared with manipulation maneuver this treatment is less traumatic, short of course of treatment, easy to perform and really an effective procedure to treat the frozen shoulder.

粘连挛缩带紧张位针刀松解治疗方法特点在于针对性地松解肩前结节间沟区、肩峰下和肩关节囊后下部等三处粘连挛缩带，在此基础上配合关节腔内注液扩张和术中术后注重肩肱关系的手法松解，与推拿治疗冻结肩相比，本法明显改善了冻结肩患者的疼痛与功能障碍程度，创伤小，疗程短，见效快，疗效可靠，且中远期随访显示疗效稳定。

Methods:keep supraspinous ligament,interspinous ligament ,thoracolumbar and the periosteum of lamina.The supraspinous ligament and interspinous ligament was cut off below affected spinal canal.The lamina was cut off longitudinally with a chisel along the outside of lamina,detach ligament flavum bilaterally,and reverse upward lamina and the compound structure of back to open spinal canal.

将部分腰背筋膜、棘上韧带、棘间韧带及椎板上骨膜保留，于病变节段下一椎间隙切断棘上棘间韧带，用骨刀（骨刀宽面与矢状面成角10~15度）在上下关节内侧2mm处自下而上并保持骨刀宽面进入椎板深度与椎板厚度相一致切断两侧椎板，牵开椎板及棘上棘间韧带，常规进行椎管减压，椎板及棘上棘间韧带回植。

MethodsThe OA rabbit model was established by immobilized in full extension for up to six weeks. Eighteen rabbits were randomly divided into the DEA group, the acupotomy group (treated with acupotomy and manipulation) and the control group (treated with manipulation alone). The treatment was applied once every three days for 1 month as a course. NO in articular synovia was measured by nitrate reduction method, and the apoptosis rate of chondrocyte was determined by flow cytometry.

方法应用关节制动的方法复制骨关节炎模型，18只造模成功的中国家兔，随机分为高频电火花水针组、水针刀组和对照组，A组用高频电火花水针加手法治疗，B组水针刀加手法治疗，C组仅予以手法治疗。1次/d，疗程为1个月，用硝酸还原法检测关节滑液中NO含量；用流式细胞仪检测软骨细胞的凋亡率。

Eighteen rabbits were randomly divided into the dea group, the acupotomy group (treated with acupotomy and manipulation) and the control group (treated with manipulation alone). the treatment was applied once every three days for 1 month as a course. no in articular synovia was measured by nitrate reduction method, and the apoptosis rate of chondrocyte was determined by flow cytometry.

方法应用关节制动的方法复制骨关节炎模型，18只造模成功的中国家兔，随机分为高频电火花水针组、水针刀组和对照组，a组用高频电火花水针加手法治疗，b组水针刀加手法治疗，c组仅予以手法治疗。1次/d，疗程为1个月，用硝酸还原法检测关节滑液中no含量；用流式细胞仪检测软骨细胞的凋亡率。

In present study, we used the adult rabbits articular chondrocytes as the object of study, we researched the effect of TGF-β1 to promote chondrocytes proliferation and preserve the synthetise of special stroma in order to provide theortically and clinically basis of culture and proliferation in vitro of a-dult people articular chondrocytes.

材料与方法 1。成年兔关节软骨细胞分离、培养：取18月龄的成年日本大耳白兔1只，处死，片状切取关节软骨，解剖刀将软骨切成1.0mm~3大小碎块，消化，过滤，离心，收集关节软骨细胞，加入DMEM培养液(含20％胎牛血清、青霉素100U／ml，链霉素100U／ml)，调整细胞密度为2.5×10~5／ml，置于37℃，5％CO_2孵育箱内培养。

Substantial for: drill, punch, tungsten steel cutter mold, stamping mold, deep drawing forming, watches shell, all kinds of knife, knife juice, stirring knives, razors, hair cutting knives, alloy knives, knife tablets, bearings, artificial joints, stamping die, gear, all kinds of precision steel parts, electronic components and automotive parts motorcycle piston ring and so on.

大量用于：钻头、冲头、钨钢刀具模具、冲压模、深拉成型、钟表外壳模具，各种餐刀、榨汁刀、搅拌刀、剃须刀、剪发刀、合金刀、刀粒，轴承、人造关节、冲压模具、齿轮、各种精密合金钢零配件，电子零件及汽车摩托车配件活塞环等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。