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CaLP's phosphorylation state analysis of different localization showed that when CaLP is overexpressed alone, it is in state of phosphorylation and stays in the cytoplasm; when CaLP is co-transfected with p21, p21 overexpression causes its unphosphorylation and translocated from cytoplasm to nucleus.

对定位于不同部位的CaLP的磷酸化状态的分析表明:当在细胞中单独表达CaLP时,它以磷酸化形式存在,且磷酸化的位点发生在Y139上,CaLP因此定位于细胞质中;当共表达CaLP与p21时,p21的过表达引起CaLP发生去磷酸化,CaLP由细胞质穿梭入细胞核并与p21发生直接相互作用。

When using restricted maximum likelihood for parameter estimation, we need to get the minimum of a function. But if the expression of the function is complicated, it is difficult to obtain the derivatives of the function. We introduced conjugate direction method which avoided the derivatives.

在用限制极大似然法估计参数时,需解决函数的极小化问题,而当函数的表达式较为复杂时,将难以获得其偏导数,本文给出的共轭方向法,避开了求导问题。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖,琼脂0.7%,pH5.8 培养基中25℃暗培养20d 后继代一次,诱导产生Ⅱ型胚性愈伤组织,用镊子将其夹碎成2mm2大小的小块,置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中,侵染时间为45min,共培养3 天后,转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天,再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中,遮光培养20d后,置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化,卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%;同时以甘蔗心叶愈伤组织材料,通过循环水式真空泵,产生负压,设定不同的负压值,在农杆菌菌液中感染5min 后,在负压条件下继续侵染15min 后,筛选出抗性愈伤组织并获得转化植株,其中在负压为-0.05 时转化率达到最大值,其卡那霉素抗性愈伤组织获得率平均为37.5%。

This is, for China and other multiracial countries, very positive and may give some solutions for the future. In the introduction there is an explanation of the reason why I choose this title and also the questions about Switzerland in the field of present research and the meaning of those researches. The first part describes the formation of the community of Switzerland, and there I define the word "community" and the peculiarity of each member of this community, the time the Swiss people take to build this community. The second part describes this formation with more details like geographical problem, language differences, the psychological pugnacity for liberty, the practical art of neutrality and the freedom for religious behavior. Those themes prove the formation of the Swiss community. The third part contains the following topics: the international position of Switzerland and its stability, the balance of economic production, the foundation of the Swiss government and the charm of its feature. For those topics I use the economic, political and cultural angle.

全文分为瑞士多民族社会的形成,在这一部分中重点介绍了民族和民族共同体的界定,瑞士各民族的地理分布及主要特征,瑞士民族共同体形成的历史时间断限,在这一部分中特别提出了民族共同体的概念,为全文的行文打下一个概念基础;在瑞士民族共同体形成的表现部分中,共分为语言和地域差别问题的消弭,崇尚独立自由的心理素质的确立,中立外交政策的奉行,宗教多元化的制衡等几个小的部分,四个部分平衡并立,共同来论证瑞士民族共同体的表现;在瑞士民族共同体诞生的原因中,共分为以下几个部分,有瑞士特殊的国际地位与多民族社会的稳定,平衡发达的经济及其吸引力,多元政治体制的建构和灿烂文化和性格的魅力,也就是从政治、经济和文化三个角度对这个问题进行了阐述;在现代瑞士多民族社会问题部分中,有如下几个部分瑞士的外来人口在瑞士国家的生活状况,外来人口融合中的困难,以及瑞士民族共同体中其他的一些社会问题,主要探讨的是瑞士社会中的外国人的生活状况,以及在融合和同化的过程中的成功经验以及给整个瑞士社会带来的几个突出的社会问题;最后是对现代瑞士多民族社会问题的思考,也就是从和睦性,认同感以及民族平等政策三个角度中进行的分析。

The solutions of ProblemⅠ,ⅡandⅢare discussed by using the generalized conjugate gradient method. When the equation is consistent, the solutions such as symmetric, skew-symmetric, centrosymmetric, centroskew symmetric, reflexive, antireflexive, bisymmetric or symmetric and antipersymmetric are successfully found; When the equation is inconsistent, the least-squares solutions such as symmetric, skew-symmetric, centrosymmetric, centroskew symmetric, reflexive, antireflexive, bisymmetric or symmetric and antipersymmetric are also found successfully. The generalized conjugate gradient method has the following traits:(1) It can judge automatically the information of solutions.

利用广义共轭梯度法,讨论了问题Ⅰ、Ⅱ和Ⅲ解的情况:当方程相容时,研究了方程的一般解、对称解、中心对称解、自反矩阵解、双对称解、对称次反对称解及其最佳逼近等问题;当方程不相容时,研究了方程的最小二乘一般解、最小二乘对称解、最小二乘中心对称解、最小二乘自反矩阵解、最小二乘双对称解、最小二乘对称次反对称解及其最佳逼近等问题。

a three steps sequence therapy was designed for patients suffered post dural puncture headache total 45 cases:step one,patient keep supine recumbence,and intravenous injection of ringer's solution 1000ml with intramuscular injection of tramadol 100mg was administrated. if pdph could not relieve within 24hr,the next step should be use. step two,drinking a cup of coffee three times a day for patients who were allowed to eating or intravenous injection ketamine 0.5mg/kg for patient in fasting diet.

选择椎管内麻醉后发生头痛的病例共45例,按设定的序贯疗法分别为a:去枕平卧1d,每天补液量增加1000ml平衡液,肌注曲马多100mg,无效时进入b和c;b:能进食者给予市售咖啡饮品,每天三次;c:不能进食者静脉注射氯胺酮0.5mg/kg,连用2d;以上治疗无效时进入d:行硬膜外填充自体血10ml。

A three steps sequence therapy was designed for patients suffered post dural puncture headache total 45 cases:step one,patient keep supine recumbence,and intravenous injection of Ringer's solution 1000ml with intramuscular injection of Tramadol 100mg was administrated.

选择椎管内麻醉后发生头痛的病例共45例,按设定的序贯疗法分别为A:去枕平卧1d,每天补液量增加1000ml平衡液,肌注曲马多100mg,无效时进入B和C;B:能进食者给予市售咖啡饮品,每天三次;C:不能进食者静脉注射氯胺酮0.5mg/kg,连用2d;以上治疗无效时进入D:行硬膜外填充自体血10ml。

Furthermore, we found that the Sema3F-triggered growth cone attraction was abolished by inhibition of the cGMP signaling pathway but not by inhibition of the cAMP signaling pathway, and the attraction was suppressed when elevating the intracellular cGMP level.

而且,在组织块共培养中,分泌Sema3F的细胞团能使小脑颗粒细胞偏向它迁移。我们发现Sema3F的吸引性作用在cGMP信号通路被抑制时受阻断,在细胞内cGMP水平增加时被削弱。

A large number of twins was found in bainitic midribs for first time. Twins in midribs as well as retained austenite are deformation twins formed by coordinating uniform shear deformation as bainitic ferrite lath grows, twins in bainitic ferritemainly are those inherited in retained austenite as BF thickens by shear mechanism in broad face.

中脊与残余奥氏体李晶是贝氏体铁素体切变共格长大时协调均匀切变形成的形变孪晶贝氏体铁素体板条内孪晶主要是其横向侧面切变增厚时残余奥氏体的遗传李晶,且孪晶面与界面台阶有对应关系。

The third part, we analyze the serial verb construction of serial action by putting it into the serial-verb-predicate pattern. According to the classifies of the tense-aspect, and in the terms of transformational generative grammar, we get 37 types, 26 sorts, 222 the serial verb construction of serial action in all. And then we summarize the freedom degree of each type of tense-aspect get into the pre-pattern and post-pattern.

第三部分,把连续动作连动式放入连续动作谓语句中进行考察,依据第二部分时体的分类,再次从转换生成语法出发,共得出了7大类26次类222个连续动作连动式句型,在这一基础之上,总结出各个时体类型进入前动结构和后动结构的自由度。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。