共价的

Centromeres could be identified easily on the bivalents at diplotene, so it was convenient to analyse the karyotype.

结果表明，日本沼虾染色体数n=52，2n=104，据双线期二价体的相对长度、着丝点位置以及形态诸方面的特点分析了核型，共分为A，B，C，D4个染色体组，核型组成是N=37M+4ST+11T；初级精母细胞减数分裂前期I可分为细线期/偶线期、粗线期、双线期和终变期5个时期，双线期的二价体存在弥散阶段，核仁明显，且双线期二价体着丝点清晰，给核型分析带来了方便。

Fermentation for 96 h in 15 L fermentor, the average yield reached to 2,103 μg/ml.

该工艺应用于15L发酵罐共十个批次的发酵试验，96h时克拉维酸的平均效价达2103μg/ml。

In the study, research aims at setting up a suitable framework to assess the farmland wetland by introducing proper wetland evaluation technology. Here, a two-stage qualitative 'multiple value evaluation matrix' is proposed and practiced for the 'Eco-park' planning at Fubao wetland in Changhua. Those functions such as sustenance, socio-education and production are approaching to the positive evaluation. On the contrary, the impacts induced by improper restoration planning on normal function of wetland are considered as negative evaluation. There are 70 indicial elements altogether, including some critical-value elements with importance meaning to the sustainable development in wetland. They are enhanced their weigh to strengthen the unique characteristic of wetland.

本研究旨在建立一个合适於农田湿地型态之评价架构，引入适当之湿地评价技术配合评估，并建立定性之两阶段多元评价矩阵评价表格，在此选定彰化福宝湿地之生态园区计画作为案例演练对象，以湿地之永续、社教及生产功能为正向评价，并参考复育计划对湿地功能的影响为负向评价，共设有70项指标性要素，并对部份要素对湿地持续发展具重要性意义者予以纳入「关键价値」要素，加强其权重来突显该湿地之特性。

The results shows that the two-way lead-lag cointegrating price relationship between milkfish wholesale and retail prices. Since the fish wholesale market could not completely and quickly assimilate the retail market information, the speed of wholesale price response to retail price changes is relatively slow, inducing the low pricing efficiency .

研究结果指出虱目鱼生产批发与消费零售价格间具有显著双向因果价格共整合关系，但生产批发价格因应於消费零售价格变动表现出较弱的价格感应度，其反应消费零售交易资讯比较不易完整且快速地传递到生产批发市场，因而造成生产批发市场相较於消费零售市场呈现较低决价效率。

The trivalent chromium passivation film based on zincate plating obtained has compact structure with no obvious crack, and excellent anticorrosion performance. Different compositions are detected from the surface to inner film, the surface mainly contains Cr2O3-Cr3-ZnO compound, the inner (1030 nm to surface) composes of Cr2O3-ZnO-Zn.The forming process of passivation film possibly consists of four steps which are zinc dissolution, basic area appearance, gelatiniform film forming and converting to passivation film.

结果表明：锌酸盐镀锌层的三价铬钝化膜表面结构致密、无明显裂纹，具有较好的耐腐蚀性能；这种钝化膜表层与里层的组成不同，表层的组成为Cr2O3-Cr3-ZnO共混物；里层(离表层10~30 nm处)的主要组成为Cr2O3-ZnO-Zn共混物；钝化膜的成膜过程可能包括镀锌层的溶解、碱性薄层的形成、胶状膜的形成和胶状膜转化成钝化膜4个步骤。

The vaccine, also called heptavalent pneumococcal conjugate vaccine or PCV7, is marketed as Prevnar in the United States and Canada and as Prevenar elsewhere in the world.

疫苗也成为七价肺炎球菌共轭疫苗或PCV7，在美国和加拿大的商品名是Prevnar，而在其他地区称为Prevenar。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Which, sGKz8 varieties in the 2003-2004 national regional trials, seed cotton, lint, lint before frost control were 41 varieties of cotton by 121.1%, 126.4%, 129.2%, ranking first in 2005 through the national approval , is the first of bivalent insect-resistant genes to the three new varieties of hybrid cotton; otherwise GKz28, sGKz6, sGKz34 combination of a total of three test are participating in the National District.

其中，sGKz8在2003-2004年全国品种区域试验中，籽棉、皮棉、霜前皮棉分别为对照品种中棉所41的121.1%、126.4％、129.2%，均居第一位，2005年通过国家审定，是我国第一个转双价抗虫基因的三系杂交棉新品种；另有GKz28、sGKz6、sGKz34共3个组合正在参加全国区试。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation.

构建了同时含有番茄儿丁质酶基因（Chi3）和β-1，3-葡聚糖酶基因的双价抗真菌基因植物表达载体，以西瓜子叶块为外植体，采用根癌农杆菌介导法，将Chi3和Glu-Ac同时导入西瓜栽培种&中育一号&，共获得46株抗性再生植株。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。