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Based on the biochemical analysis of the two proteins in vitro, the results of yeast two-hybrid experiment and protein-interaction in both wild type cells and C48 strain, we conclude HetR can form a homodimer through the Cys48 residue. We also show that the dimerization is required for heterocyst differentiation.

基于体外两种蛋白的性质比较和酵母双杂交的结果,并结合相应蓝细菌野生型和突变株C48A中HetR蛋白性质的体内研究表明:HetR蛋白可以通过第48位半胱氨酸残基形成共价结合的同源二聚体,该同源二聚体是异型胞正常分化所必需的。

The structure reveals the catalytic intermediate of DAPDC, in which L-lysine is covalently bonded to PLP to form an external aldimine. This conformation provided the best insight to date on the substrate binding and catalytic mechanism of DAPDC. It also supplied valuable clues for designing specific DAPDC inhibitors.

通过分析我们发现这是DAPDC酶家族的第一个反应中间态结构,即反应产物L-赖氨酸与辅酶PLP共价结合形成了外源醛亚胺的构象,这为了解DADPC的底物结合情况及催化机理提供了迄今为止最详细深入的结构信息,也为针对DAPDC设计特异性抑制剂提供了宝贵的线索。

The conjugation of MMS and Ab (ready for Reagent 1, R1): Chose the 3μm diameter Carboxyl MMS and use the carbodiimide cross-link technique activate the MMS with EDC and NHS in different concentration, then linked mice anti-HBsAg-IgG and mice anti-HCcAg-IgG on them.

磁微粒的活化及其与抗体的连接:选择粒径3μm的羧基化磁微粒,采用碳二亚胺共价交联法,在活化剂EDC与NHS的不同浓度组合条件下活化磁微粒,选择最适的活化剂浓度组合。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面,本文在优化并确定了多基因PCR反应条件的基础上,采用了两种方法对多基因PCR产物进行鉴定:①采用共价结合方法将多个探针固定在Biodyne〓C膜上,将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应,首次建立了GMO的多基因PCR-膜相杂交检测方法,实现了一个反应体系中同时检测多种转基因成分的目标,提高了检测效率与准确率。

In order to mimic this repair process of apoenzyme, we synthesized two model compounds, in which tryptophan and/or tyrosine was covalently linked to cyclobutane thymine dimer, and measured the splitting quantum yield of dimer under 295 nm light. By investigating the photosensitized splitting properties of the dimer, some new insights into the intramolecular electron-transfer process were gained.

为了模拟酶蛋白的这一修复过程,合成了色氨酸和/或酪氨酸与胸腺嘧啶二聚体共价连接的化合物,作为酶-底物复合物的模型,研究了它们在295 nm光照射下氨基酸残基光敏化二聚体裂解的性质,测定了二聚体裂解量子产率,获得一些新的结果并对其进行了分析。

On the bases of a solid solution hardening model, and considering the solubility of isoelectronic impurities in InP crystals and the elastic misfit arised from the differences of the tetrahedral radii between added impurities and host atoms, the pinning forces as large as 〓 have been obtained for isoelectronic impurities in InP.

以InP单晶中的等电子杂质为例,根据固溶强化模型,考虑了等电子杂质在InP单晶中的溶解度以及掺入杂质与基质原子之间共价半径之差引起的弹性失配,计算了这些杂质在InP中对位错的钉扎力可达10〓~10〓达因/厘米〓,从而很好地解释了等电子杂质降低位错密度的作用。

Depending on the amino acid sequence, the length of the ∝-helical portio are held together by hydrogen bonds formed between r grou , by salt linkages, by hydrophobic interactio , and by covalent disulfide(-s-s-0 linkages.

根据氨基酸顺序的不同,此α-螺旋段的长度也不同,并赋予独特的三级结构,这些折叠部分是靠R基团之间所形成的氢键,靠盐键、疏水相互作用以及共价二硫键而结合在一起的。

The quantitative relationship between isomer shift, refractive index and chemical bond parameters were established. The calculated results indicated that the change of crystal parameters and bond length will alter the covalency of chemical bond and peripheral electron density of Fe2+ nuclear, which in turn leads to the change of the isomer shift and refractive index.

根据计算结果发现,晶胞参数及化学键长的变化引起化学键共价性、Fe2+核附近电子云密度的变化等,最终导致同质异能位移的改变,计算结果与实验数据之间较好的一致性验证了此理论的准确性和普适性。

This paper compared the Covalency - Effect and the Nephelauxetic - Effect, applied these two methods in the absorption spectra of DMS Zn1- xMnxTe, and found that both methods can gain fine results. But the results by applying the Nephelauxetic - Effect modifying factor to modify the radial wave functions are much closer to the experimental values.

该文对共价效应和电子云延伸效应在处理稀磁半导体的吸收光谱方面的应用做了详细的比较,并分别应用到了稀磁半导体Zn-xMnxTe上,虽然两种处理方法都能够得出比较好的结果,但用电子云延伸效应修正因子修正波函数得出的结果与实验更为接近。

Adding transition metals into the TiN films by arc ion plating (1) The hardnesses of theN films are higher that that of the TiN films, proving that the alloy hardening effect is a common phenomenon;(2) The structures of the alloyed films are determined by the added element properties such as covalent radius, electro negativity, free energy and lattice parameters of the MN compound;(3) theN film consists of a mixture of multi phases with high hardness but quite brittle, while the other MN films are composed of single phase with the fcc TiN structure and their hardesses, functions of the added amounts of the elements, manifest always a maximum at a certain M percentage.

一、将过渡金属M用电弧离子镀方法添加于TiN薄膜中:1。N薄膜硬度均高于TiN薄膜硬度,合金强化效应普遍存在;2。薄膜相结构决定于添加元素M的周期律信息,包括M的共价半径、电负性,及MN化合物的自由能、晶格常数等;3。N为多相混合结构,薄膜硬度高,但韧性差;其它N的相结构为TiN类型的单一fcc结构,薄膜硬度随M添加量变化并出现峰值;4。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。