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To further investigate their roles in cell proliferation apoptosis and differentiation, three human leukemia cell lines (K562, U937 and HL60) exposed to various concentration of hexamethylene bisacetamide, a potent inducer of differentiation of transformed cells both in vivo and in vitro, were used as an in vitro model for the study of cell proliferation, differentiation and apoptosis.

为了进一步研究cyclin A和p21在白血病细胞增殖,分化,凋亡中的作用,我们用在体内,外均有抗肿瘤作用的药物六亚甲基二乙酰胺在体外抑制白血病细胞株K562,U937和HL60增殖,诱导其分化,成功地建立了与药物干预浓度呈剂量-效应关系的,具有不同增殖,分化,凋亡状况的实验细胞模型。

Chinese patent medicine ; Zilongjin ; hexamethylene bisacetamid ; cell cycle ; oncogenes ; genes ; tumor suppressor

中成药;紫龙金;环六亚甲基二己酰胺;细胞周期;癌基因;基因;肿瘤抑制

In this paper,we used hexamethylene bisacetamide as a inducer to induce human mucoepidermoid carcinoma cell line MEC-1 cells to differentiate in vitro and vivo.The nucleolus organizer regions was investigated by means of the argyrophil technique.

本实验以六亚甲基二乙酰胺作为分化诱导剂,对涎腺粘液表皮样癌MEC-1细胞进行了体内外诱导,并通过银染技术对对照组和HMBA诱导组癌细胞核内核仁组成区的数目、大小及分布特征进行了观察。

When 45% methanol solution containing 0.5 mmol/L hexamethylene tetraamine was used as the pump carrier, the pump flow rate and output pressure achieved 0.58 mL/min and 0.79 MPa at 4950 V, respectively. By using the pump carrier as the mobile phase, benzene and naphthanlene were separated on baseline by the EFA system.

在4950 V的外加电压下,以含0.5 mmol/L六亚甲基四胺的45%甲醇溶液为泵载流,电渗泵的流量和输出压强分别为0.58 mL/min和0.79 MPa,以泵载流为流动相,该系统对苯和萘进行了反相色谱分离。

Objective To investigate the effects of the Chinese drug Zilongjin and hexamethylene bisacetamid on expression of oncogenes(c-ras,c-myc),and tumorsuppressor genes(Rb,p53,p21)of MGc803 cell in cell cycle.

目的 探讨中药紫龙金与环六亚甲基二己酰胺对人胃癌MGc80 3不同周期细胞癌基因c ras、c myc、抑癌基因Rb、p5 3、p2 1以及蛋白激酶PKC a基因表达的影响。方法采用Northernblot印迹法检测基因表达情况。

Jinzhou Chemical landing six branches now have the world's larger 5,000 tons / year, Central Sulfolane a plant to produce 1,500 tons mainly isopropylamine / devices in a production, domestic production of methyl iso unique ---A, two isobutyl ketone and methyl isobutyl ketone methanol mainly 1,500 tons / year of a production plant.

锦州六陆化工分公司目前拥有世界上较大的5000吨/年环丁砜生产装置一套、以生产异丙胺为主1500吨/年生产装置一套、国内独有以生产甲基异丁基酮甲酮、二异丁基酮和甲基异丁基酮甲醇为主1500吨/年生产装置一套。

Models of K562 cells inducing differentiation to lineages of megakaryocyte, erythrocyte and macrophagocyte were established with inducers TPA (tetradecanoylphorbol 13-acetate), Hu and HMBA respectively.

用Western blot检测以十四烷酸佛波醇-13-乙酯(tetradecanoylphorbol 13-acetate, TPA)、羟基脲(hydooxyurea, Hu)及六甲基双乙酰胺(hexamethylene bisacetamide, HMBA)为诱导剂分别诱导K562细胞向巨核细胞系、红细胞系及巨噬细胞系分化时bcl-6表达变化,用PCR、克隆、直接DNA测序方法分析bcl-6基因5'调控区序列变异情况。

Results Shuichangpu increased the resting tnesion of longitudinal and cicular muscule of fundus and bodys.Atropine and hexamethonium inhibited the effect of shuichangpu partially.

结果 水菖蒲水煎液增高胃底和胃体纵、环行肌条的张力,阿托品和六烃季胺部分阻断水菖蒲增高胃底纵、环行肌及胃体环行肌张力的作用。

The bonds of nitrogen-nitro mainly affect the sensitive of blast and the stability of Nitrogen heterocyclic nitramine.

另外对DNDC和NPI这两个同属于六元环的氮杂硝胺类的炸药分子进行计算研究。

Considering the low morphological stability of dye-doped polymer thin film and based on the above multi-chromophoric organic small molecules, six novel copolymers have been synthesized by the co-polymerization, in which there are three functions: 1, 8-naphthalimide as emitting moiety, oxadiazole as electron transporting moiety, and N-vinyl carbazole as hole transporting moiety.

考虑到有机小分子掺杂到聚合物膜中低的形态稳定性,我们在上面多发色团有机小分子研究的基础上,通过自由基引发共聚合成了六个新型的共聚物。在该类共聚物的侧链上含有三个功能团:1,8-萘酰亚胺单元、噁二唑单元和N-乙烯基咔唑单元。

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Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在,所有的孩子都要在综合学校继续学习,所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地,使人们普遍认识到发展不能以牺牲农业为代价。

Detailed Intrinsic Reaction Coordination calculations were carried out to guarantee the optimized transition-state structures being connected to the related tautomers.

同时,做了详尽的内禀反应坐标计算,以保证所得到的过渡态连接相应的始末异构体。