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The results showed: supplementing fat emulsifier(0.25% MeiFei)selected to diet in broilers, slaughter performance of broiler was obviously increased, upon the same fat concentration, the slaughter rate was increased by 0.79~1.38%, the eviscerated yield with giblet was increased by 1.30~2.62%, and the eviscerated yield was increased by 0.89~1.55%;the abdominal fat was decreased by 0.20~1.88%; The emulsifier excelled volamel in effect of on performance of broiler.

本次试验在23—54日龄肉鸡日粮中添加脂肪乳化剂—0.25%美肥和不同浓度的脂肪,并在日粮中添加脂肪乳化剂—0.05%唯乐美作为比较。结果表明:所选用的乳化剂可显著提高屠体重,半净膛率和全净膛率,降低腹脂率。同种脂肪浓度下,美肥添加组的屠宰率比未添加组高0.79~1.38%;美肥添加组的半净膛率比未添加组高1.30~2.62%;美肥添加组的全净膛率比未添加组高0.89~1.55%;美肥添加组的腹脂率比未添加组低0.20~1.88%;且其效果总体上优于唯乐美。

In Hangchow it has four generations each year and the time of its occurrence is well synchronized with that of the scale.

根据测定,隐斑瓢虫对日本松干蚧各虫态的捕食能力较强,一头成虫的日平均捕食量为:显露若虫13.33头,雌成虫4.27头,卵囊3.67个(每个卵囊内平均有卵265.5粒),雄蛹28.27只;一头幼虫全期(或2—4龄和3—4龄)分别能捕食显露若虫39.20—105.56头,雌成虫40.80—47.79头,卵囊39.38—41.79个,雄蛹220.50只。

The case presentation is a 70 year-old male patient who sought medical attention because of odynophagia and a right neck mass. He had a history of smoking for 50 years. Initial biopsy result was an undifferentiated carcinoma and he received a total laryngectomy combined with partial pharyngectomy and pharyngeal reconstruction and radical neck dissection. The final pathologic diagnosis is small cell carcinoma. He was then given post-operative radiotherapy. More than two years of follow-up shows no recurrence or distant metastases.

本院於2001年8月曾经治疗过1个下咽部小细胞癌之患者,他是一个菸龄50年的70岁男性,因为吞咽疼痛及右侧颈部肿块而来就诊,经切片检查结果为未分化癌,於是住院接受全喉切除并部份咽部切除与重建和右侧根除式颈部淋巴廓清术手术,手术后病理报告证实为小细胞癌,术后并接受放射治疗,追踪至今2年多,并无复发及远处转移的现象。

These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设,我们设计了10种培养条件,在自行设计的无血清培养基中添加不同的细胞分化诱导物,包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等,分离孕10周龄人胎肝细胞进行原代培养,观测肝细胞形态及功能指标变化,并定时给予HBV感染,观察能否在体外诱导胎肝细胞出现HBV易感性;为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA),我们改进了现有的PCR检测法。

Methods The discharge features of hippocampus CA1 pyramidal neurons of 14-day-aged healthy Sprague-Dawley rats induced by low dose (0.3~0.8mol/L) of veratrine were observed by slice patch-clamp technique.

应用脑片膜片钳全细胞记录技术,观察小剂量(0.3~0.8mol/L)Ver灌流后SD大鼠(2~3周龄,共30只)海马CA1锥体神经元的放电特征。

First 27 rabbits of the experimental group were destabilizated by operated on L6、7.Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射麻醉后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射####后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

To acquire more information about gene expression at the whole-genome scale,our lab and National Engineering Center for Beijing Biochip Technology worked together and constructed the first genome-wide oligonucleotide microarray of the silkworm.This microarray was used to detect gene expression of 10 tissues/organs,including midgut,integument,head,hemocyte,testis,ovary,anterior/median silk gland,posterior silk gland,fat body and malpighian tubule.In this study,we performed analysis of these microarray data and confirmed the reliability of the results.

为了在全基因组水平上获得基因表达信息,本实验室与生物芯片北京国家工程中心合作,共同定制完成了世界上第一张家蚕全基因组寡核苷酸基因芯片,并利用该芯片对家蚕5龄第3天幼虫的中肠、体壁、头、血液、精巢、卵巢、前中部丝腺、后部丝腺、脂肪体、马氏管这10个组织中的基因表达谱进行了检测。

Of the 19 cases,19 fetus were found,MRI confirmed 14 cases considered or suspected as fetal corpus callosum agenesis by US(8 cases are completed agenesis of corpus collosom-CACC,6 cases are partial agenesis of corpus collosom-PACC, and 5 cases had took on autopsy),however 3 cases are mild enlargement of lateral cerebral ventricle and 2 cases are leukodystrophy.In these cases,MRI confirmed a Dandy-Walker syndrome and a lipoma of corpus callosum suspected by US,detect a Dandy-Walker syndrome and a microcephalus made missed diagnoses by US.The fetal corpus callosum and additional cerebral anomalies were shown more clearly on MR T_2-weighted images.

胎儿胼胝体长度与额枕径的比值在24~36孕龄阶段相对较恒定。2、19例孕妇共检出胎儿19个,MRI证实超声疑诊的胎儿胼胝体发育不全14例(完全型胼胝体发育不全8例,部分型胼胝体发育不全6例,有胎儿尸检结果对照者5例),3例为单纯侧脑室轻度扩张,2例考虑脑白质发育不良,合并Dandy—Walker综合征1例,胼胝体脂肪瘤1例,检出超声漏诊合并Dandy-Walker综合征1例及脑小畸形1例。

Results Mean number of errors of the mice were 1±0.3, 0.9 ± 0.6 and 1.4±1.0 respectively, all of them were different significantly compared with control on the second, third and fifty-sixth day after the brain irradiated with 20Gy electron beam.

硫喷妥钠麻醉后,采用直线加速器10MeV电子线照射136只4-6周龄昆明鼠全脑,四组动物分别给予20Gy、10Gy、5Gy、0Gy,照射后第1、2、3、4、5天和第14、28、56、84天采用跳台法测定鼠的学习记忆力。

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