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The results exhibited that, the cDNA gene entailed 2740bp, had single copy in the rice genome and encoded peptide of 765 amino acids, the peptide showed 92% and 83% identity with that from Mesembryanthemum cystallinum (U84889) and Cathararanthus roseus (X83499), respectively.

结果表明,VB12不依赖型蛋氨酸合成酶cDNA基因全长为2740bp,在水稻基因组中以单或低拷贝存在,编码765个氨基酸,与 Mesembryanthemum cystallinum (U84889)和 Cathararanthus roseus (X83499)的同源性分别为92%和83%。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因,并进行序列分析。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因,并进行序列分析。

The ORF of profilin of Caryota mitis pollen was amplified by RTPCR using specific primers and cloned into the expression vector pET-28a.

结果克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

At water temperature of 16.0-17.2 ℃, the process of embryonic development could be divided into 24 stages, which were described as follow: fertilized egg at 0 min, 2-cell stage at 75 min post fertilization; myotome forming stage at 43 h PF, and hatching-out stage at 94 h PF when the accumulative temperature reached 1616.8 ℃.h, and the average size of larvae was about 3.8 mm in body length.

梭鲈胚胎发育过程可分为24期,在水温16.0~17.2℃范围内,受精75 min开始第1次卵裂,受精后43 h开始形成器官,受精后约94 h开始出膜;刚出膜的仔鱼全长平均为3.8 mm;从受精到孵化出膜总积温为1 616.8℃。h。

Changhong Road Food Street with Jiqingmen Street in its southmost and Shuiximen Street in northmost, is 1.2 kilometers long and 40 meters wide commercial food street, integrating culture, catering and water relaxation.

长虹路美食街南起集庆门大街,北至水西门大街,全长1.2公里,路幅宽40米,是一条集文化、餐饮、亲水休闲于一体的美食商业街。

Objective: To clone the complete gene of nucleoprotein of H3N2 subtype avian influenza virus, and to express recombinant NP in E.coli for the function research.

目的:获得H3N2亚型禽流感病毒核蛋白全长基因,并在大肠杆菌中表达,以用于对NP功能的研究。

Five pieces of ESTs showed strong homology to it, and they were assembled to one 772bp cDNA sequence by DNASTAR software.

结果显示ECRG-4近似cDNA全长序列为772bp,其中含有一447bp的完整阅读框,编码148个氨基酸。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒,并与pCDNA3质粒,按3:1比例混合后,运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO),用新霉素类似物G418初步筛选出阳性克隆。

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