全长的

The phylogenetic analysis of Gymnothorax was reported in this paper by being based on DNA sequences of mitochondrial Cytb gene.17 species of Cytb gene full-length sequences were obtained and their length of Cytb gene was 1140bp.All sequences were analysised by using biological software, the phylogenetic tree was constructed by using three kinds of common contribution phylogenetic tree methods and the phylogeny of 17 species of Gymnothorax was deduced.

本论文采用线粒体Cytb基因为分子标记，共获得裸胸鳝属鱼类17种Cytb基因序列全长为1140bp的部分序列，应用分子生物学软件对结果进行分析，使用3种常见的建树方法：NJ法、ME法和MP法重建系统发育树，对裸胸鳝属17种鱼类的系统关系进行研究，为探讨裸胸鳝属鱼类的分子进化，确定它们之间的关系提供分子生物学方面的证据。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性，研究结果表明含有第1～685位和含有第22～655位氨基酸的蛋白片段对小菜蛾的毒性，与全长Cry1Ba3蛋白相比，没有改变；含有第54～655位氨基酸的蛋白片段对小菜蛾的毒力明显降低；而含有第22～627位和含有第85～655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

The superstructure uses the standard drawing, the substructure has used the gravity T-shape abutment and the rectangular buttress, in the design carries on the size to the abutment and the bridge pier to draw up, simultaneously satisfies the design the principle, the abutment uses 9.3m, in satisfies the structure and under the operation requirements premise, the abutment uses 9.3m to be long, because the geological condition decided, the pillar body uses 12m to be high, the open excavation foundation, the process load computation and the load combination, has carried on the bridge abutment and the pillar bottom section stress separately to the abutment and the bridge pier examines calculated, examines calculated the result conforms to the standard requirement, the bridge span uses the straight line build, and established this bridge construction organization plan, including the engineering project characteristic, the construction plan, the job practice, the bridge building construction technology, the measure has drawn up the top cap and the tray structure and matches the muscle chart, finally has drawn up Shui Xigou the bridge overall and the horizontal plan.

内昆铁路水溪沟大桥，中心里程为DK608+393.00，该桥的设计方案有三个，通过技术、经济比较，采用4×32m预应力混凝土简支梁桥方案，桥梁全长149.5m，I级单线铁路，设计时速为120km/h，设计荷载为中活载。上部结构采用标准图，下部结构采用了重力式T 形桥台和矩形桥墩，设计中对桥台和桥墩进行了尺寸拟定，在满足构造和使用要求的前提下，桥台采用9.3m长，由于地质条件决定，墩身采用12m高，明挖基础，经过荷载计算和荷载组合，对桥台和桥墩分别进行了台底和墩底截面应力的检算，检算结果符合规范要求，桥跨采用直线布设，并且编制了该桥的施工组织设计，其中有工程项目的特征、施工方案、施工方法、桥梁工程施工技术，措施绘制了顶帽及托盘构造及配筋图，最后绘制了水溪沟大桥的总体及平面图。

Originally, the fourth day's tour was planned to ride from Tainan to Fong Bi Tou Harbor, a 71-kilometer ride. However, because the riders felt stronger and more used to long distance riding after the first three days' cycling accomplishments, they decided to change today's finishing point to Dapeng Bay National Scenic Area in Donggang Township, an 82-kilometer ride.

进入第四天的A-Team自行车环岛行程，由台南市出发，原订终点为凤鼻头渔港，全长71公里，不过考量到车队成员们经过3天的磨练之后，体能逐渐上升，也习惯了长途的骑乘，71公里的路途稍嫌太短，於是临时将当天的终点拉远至东港的大鹏湾国家风景区，全程共82公里。

Tainan City, Taiwan –Coming to National Cheng Kung University, if you want to plan a full day long trip, you definitely cannot miss the nearby well-known tourist attractions and historic sites. Such as Dasyue Road, Yule Street, Shengli Road and Changrong Road are excellent must-visit places for a variety of delicacies with reasonable price. Of course, if you have enough energy, you can also challenge yourself riding on a total of 11.9 kilometres long bikeway. Let's go on an environmental-friendly and substantial cycling trip!

来到成大附近想要省荷包规划一趟充实的一日游行程，除了不能错过成大校园内的著名景点与古迹，校园周边的大学路、育乐街、胜利路、长荣路，也是您寻找平价美食不能错过的必游之地，当然，体力够的话也可以往外延伸，一口气骑完全长共11.9km的美丽公园自行车道，让您来一趟既环保又充实的省钱之旅逍遥游。

The giant arching sweep of the new terminal at Beijing Capital International Airport, designed by Britain's Norman Foster, is the first thing that will greet most Olympic arrivals. T3 is one of the world's largest enclosed spaces, designed to handle an estimated 50 million visitors a year: It is just over two miles long, with 101 gates, 31 miles of baggage conveyers, 98 moving walkways, 168 escalators and 179 elevators.

对大多数为奥运而来的宾客而言，最先映入眼帘的将是由英国的诺曼·福斯特设计的北京首都国际机场新航站楼那宏伟的穹拱形屋顶。3号航站楼是世界上最大的封闭空间之一，年接待量预计将达5，000万人次：航站楼全长约两英里，有101扇门，31英里长的行李传送带，98条电梯步道，168部自动扶梯和179部电梯。

The giant arching sweep of the new terminal at Beijing Capital International Airport, designed by Britains Norman Foster, is the first thing that will greet most Olympic arrivals. T3 is one of the worlds largest enclosed spaces, designed to handle an estimated 50 million visitors a year: It is just over two miles long, with 101 gates, 31 miles of baggage conveyers, 98 moving walkways, 168 escalators and 179 elevators.

对大多数为奥运而来的宾客而言，最先映入眼帘的将是由英国的诺曼·福斯特设计的北京首都国际机场新航站楼那宏伟的穹拱形屋顶。3号航站楼是世界上最大的封闭空间之一，年接待量预计将达5，000万人次：航站楼全长约两英里，有101扇门，31英里长的行李传送带，98条电梯步道，168部自动扶梯和179部电梯。

The full-length cdna of bin2a is 2606bp, encoding 637 amino acid. its amino acid sequence has nine conserved blocks of glycosyl hydrolases family 35, including an active site glu210 of proton donor, but lacking another active site glu279 of nucleophile.

bin2a的全长cdna为2606bp，编码×××个氨基酸。bin2a的氨基酸序列中包括glycosyl hydrolases family ×××家族中的9段相对保守的序列，其中包括210位的活性中心glu，但是缺少另外一个活性中心，279位的glu。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新