全长的

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA，并采用RT-PCR方法，根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物，克隆了含开放阅读框的生长激素(Growth Hormone，GH)cDNA序列，测序结果显示，高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp，编码140-170个氨基酸的成熟GH多肽片段；从漠斑牙鲆和大菱鲆脑垂体中提取mRNA，利用SMART-RACE技术建立脑垂体cDNA文库，并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

Sequences analysis revealsthat these 54 strains have as high as a 99% similarity in 16S rRNA gene level. Allthese 54 strains could be classified into six subgroups according to six differentregions on full-length 16S rRNA gene. Even if the regional differences are not evidentto distinguish B. thuringiensis from B. cereus, however the data of the 16S rRNA genesequences will serve to broaden the database for Bacillus, and provide someinformation for the classification of genus of Bacillus. Additionally, seven B.

本研究利用通用引物，对54株苏云金芽胞杆菌和蜡状芽胞杆菌的16S rRNA基因进行测序，并对所得到的序列进行简单的分析，发现苏云金芽胞杆菌和蜡状芽胞杆菌的16S rRNA基因的相似性很大，大约为99％，在其全长含有6个区域的不同，根据这6个区域将Bt和Bc分成6个亚群，而Bt和Bc在不同亚群之间交替存在，说明16S rRNA基因上的差异不足以区分Bt和Bc。

Integrated with the calculation methods of soil pressure, the mechanism of soil pressure of fale is studied systematically and detailedly based on the mode of axial stress distribution of wholly grouted anchor.

在全长粘结式锚杆拉力分布模型基础之上，结合本文的土钉土压力计算方法，本文对面层土压力机理进行了系统详细的研究，提出了面层局部土压力、面层土压力及土钉最大拉力的计算公式和分布模型，理论分析和实例分析表明，本文提出的轴力分布模型和面层土压力机理是合理的，解决了面层土压力取值标准问题，为面层设计提供了定量的依掘，土钉最大拉力可直接用于土钉长度设计。

Homology analysis showed that the sequence of GoCV-yk01 had 91%-93 % similarity to that of Taiwan and Germany strains.

基因组序列分析表明，浙江永康株GoCV-yk01全长1821bp，具有圆环病毒共同的与病毒复制相关的茎环结构和Rep蛋白保守基序等特征，它与德国、中国台湾发表的序列在全基因组水平有91%～93%的同源性，在Rep和外壳蛋白的氨基酸水平有94%～97%的同源性。

The full-length cDNAs of rice and wheat S-adenosylmethionine decarboxylase gene were isolated and characterized. The rice SAMDC cDNA is 1560 bp in length, encoding a polypeptide of 398 amino acids, and the wheat SAMDC cDNA is 1351 bp in length, encoding a polypeptide of 392 amino acids. Both the rice and wheat SAMDC proteins contain the conserved proenzyme cleavage site and the PEST domains, which are commonly found in the SAMDC protein sequences of all eukaryotes.

克隆了水稻和小麦SAMDC基因的全长cDNA序列，所获得的水稻SAMDC基因序列长1560 bp，编码一个由398个氨基酸组成的多肽，小麦SAMDC基因序列长1351 bp，编码一个由392个氨基酸组成的多肽，二者均含有真核生物SAMDC蛋白所拥有的两个保守的氨基酸结构域：酶原剪切位点和PEST结构域。

The result of whole mount in situ hybridization showed that MmeFer was located at the position of shell initiation in trochophore stage, indicating MmeFer plays a role in shell initiation in M. meretrix. The full-length of M. meretrix cathepsin B cDNA was cloned with 3'and 5'RACE. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. From L2 to L4 stages, MmeCB mRNA was detected in the digestive tract, suggesting a possible role of MmeCB in digestion.

根据构建的文蛤幼虫cDNA文库中提供的序列信息，从文蛤中克隆了与铁离子代谢密切相关的铁蛋白的全长cDNA序列；通过Real time PCR发现，MmeFer mRNA的表达量在贝壳形成前后有明显改变；整体原位杂交结果显示MmeFer mRNA在L1期的表达部位刚好是贝壳生成的起始部位，推断文蛤铁蛋白与文蛤幼虫贝壳初始形成密切相关。

The National Institute of Agrobiological Sciences in Tsukuba, Japan, has accumulated numerous rice biological resources and has already successfully produced a high-quality genome sequence, a high-density genetic map with 3000 markers, 30 000 full-length cDNAs, oer 700 expression profiles with a 9000 cDNA microarray and 15 000 flanking sequences with Tos17 insertions in about 3765 mutant lines from about 50 000 transposon insertion lines.

日本的NIAS（National Institute of Agrobiological Sciences ）已经积累了大量的水稻生物学资源，并成功完成水稻全基因组的测序工作，构建了3000个marks的高密度遗传图谱，收集了30000条全长cDNA序列、超过含有9000cDNA条cDNA microarray的700个表达谱，和Tos17插入大约50000个转座子插入系中的3765个突变系的15000条侧翼序列。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法，以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白，获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid／lymphoid or mixed-lineage leukemia 3)等几个候选蛋白，其中NAC1、MLL3与基因的转录调控有关，DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关，而BicD2与高尔基体的调控紧密相关，提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.

测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新