全长的

Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

Cylinders with a full-length eductor tube, or what is sometimes called a full-length dip tube, have a tube that runs from the inlet of the cylinder valve to the bottom of the cylinder .

带有全长排放管，或者有时称为全长汲取管的钢瓶有一个从钢瓶入口一直伸到钢底部的管子。

The venom of elapid snakes contains a number of small proteins that display a broad spectrum of pharmacological activities.In study on the neurotoxin from the venom gland of Bungarus multicinctus,five cDNA sequences (MNCTL1-a,MNCTL1-b,MNCTL1-c,MNCTL1-d and MNCTL2) encoding two novel proteins were obtained from the total RNA by reverse transcription-polymerase chain reaction.

采用SMART技术和RT-PCR方法，从同一个银环蛇毒腺中克隆到了5 种尚未报道过的心脏毒素类似物全长cDNA序列（MNCTL1-a、MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2）。5种序列中，MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2的长度均为505 bp，包括5′非翻译区32 bp，3′非翻译区212 bp和其余261 bp组成的一个完整开放阅读框；MNCTL1-a 由于3′非翻译区24 bp的缺失，全长cDNA仅为481 bp。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA，其全长为2421bp，开放阅读框为1416bp，编码471个氨基酸，与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Ssp. pekinensis Olsson. cv. Huangya-14, Raphanus sativus L.cv. Yuanbai and Orychophrogmus vi-olaceus L.

品种圆白和诸葛菜Orychophrogmus violaceus）3个材料中扩增到3个完整的cDNA全长，分别命名为BcCYP86MF，RsCYP86MF和OvCYP86MF，它们的cDNA全长分别为1 854，1 818和1 876 bp，分别编码524，526和534个氨基酸残基。

The length of hand and width of palm and the height in 569 young people (young women=289, young men=280) were measured and the data were analyzed regressively by digital computer.

对569名青年（男＝280，女＝289）进行了手全长、手掌宽和身长的测量，并将测量数据输入计算机中进行回归分析，得出手全长、手掌宽推测身高的直线回归方程。

The main construction task includes (1) total length is 3.5km modification project of trunk canal of Xiao River in the Private Operated Land, drainage cross section is 2.5×3m spear plate culvert,(2) infrastructure of the road drainage and protecting against flood of both ends of Huitong Road and Medicine Land B area, total length is 6000m.

项目建设规模及内容：项目计划占地面积100亩，主要建设内容包括⑴民营园内潇河干渠的改造工程全长3.5公里，排水断面2.5 X3M的盖板涵。⑵汇通路两侧及医药园B区的道路排水及防洪设施，全长6000 米。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了