全长的

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因，鉴於intersex在果蝇中所扮演性别决定的重要功能，对於此基因在鲤鱼中所担任的功能而感到兴趣，为了进一步探讨cIX的功能，本论文完成cIX全长cDNA，设计并建构cIX的重组DNA，再利用纯化之重组蛋白诱导抗体。

High strength AI extruded profile for convenient installation; AI house with reliable continuity at whole length; Double heads and single bolt joint pack; High conductivity Cu or AI bars; Outlet device with patent; High protection grade--IP65 devices; Tap-off box with interlock and operating handle

方便安装的高强铝合金材料；全长可靠连通的铝合金；双头力矩单螺栓连接器；高导电率的铜或铝导体；专利技术的插口装置；高防护（IP65）装置；带操作手柄和机械连锁的插接箱

Girth flange bolting shall be of stud bolt type, threaded full length, and at least M16 for flange of 600 mm and smaller inside diameter and at least M20 for over 600 mm inside diameter flange.

翼缘法兰螺栓应为双头螺栓，螺纹为全长型，内径600mm 或以下的法兰的螺栓至少为 M16，内径600mm 以上的法兰的螺栓至少为 M20。

These two varieties with strong salt-tolerance are suggested being used directly for wheat production in salinity regions.2. Measurement of some physiological factors related to salt-tolerance in wheat cultivars SW12Using HPLC-ESI-MS technique, Glycinebetaine, proline and malondialdehyde in leaves, which collected from wheat cultivars SW12, Ningchun 4 and Chinese Spring exposed to salt treatments with concentration ranges of NaCl s of 0, 100, 200, 300, 400 and 500 mmol/L and sampling for 1, 3, 5, 10, 20 and 30 days, respectively, were measured.

中国农业科学院博士学位论文摘要3、小麦SW12耐盐相关基因Ta一LDH的克隆利用eDNA一AFLP和RACE等技术，从430 mmol/L盐胁迫下的小麦SW12中首次分离出了一个醛脱氢酶类似基因的全长eDNA，命名为儿胡LDHoTa一乙DH含有1 948个核营酸，编码一条由509个氨基酸组成的多肤，在其3'端，距离终止子约235 bp处有一个明显的加尾信号序列。

RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果：分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体；在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS摘要: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

方法摘要：运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

The Dunes is the 18 hole championship course has been designed to exploit the natural beauty of the stunngly beside the Bass Strait.

The Dunes有18洞的锦标赛球场，它球道设计围绕自然的风光景色，全长6409米的球道，高低起伏独有的特色带来趣味同挑战。

RESULTS: The nucleotide sequence was consistent with that of HBV adr subtype PreS gene.

结果：成功地扩增到 Pre S区全长基因，测出的序列与已知的HBV 病毒的adr 亚型的前 S区基因序列一致。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新