全长的

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中，利用PCR克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码PPT乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。

Based on the correlation analysis between Bouguer anomalies and station elevations, nine terrains and thirteen large faults were recognized.

中文摘要：作为亚东—格尔木地学断面研究项目的一个重要组成部分，于1987年完成了亚东一格尔木及东拉一尼木的重力补点测量1：50万）工作，共布设了226个测点，全长1670 km在这些测量数据的墓础上，计算了研究域内的莫霍深度和均衡异常，分析了地壳和上地幔的均衡信息。

BUTCH:"Worst... The worst was also the funnest,'cause I got up early Thanksgiving day and we went to the Burwick Marathon, but it's a nine-mile road race. Just a crusher."

巴奇：最糟糕的……最糟糕的一次也是最有趣的一次，因为感恩节那天我起床很早，我们去搞柏威克马拉松比赛，但全长只有9米远，一下子就跑完了。

Expression vectors pET-TPO and pBV-TPO were constructed to express full-length TPO cDNA in E.coli . Influence of SD-AUG and promotors on the expression level was studied.

在克隆并分析了TPO cDNA的基础上，将全长hTPO cDNA克隆到原核表达载体pET-15b和pBV220中得到了表达，并且以pBV220为载体探讨了SD序列与AUG之间的距离对cDNA表达效率的影响；为了进一步提高表达效率，将hTPO cDNA 5？

Waste water drainage system of Longhu Street, total length is 3.7km, drainage cross section is 2.5×3m spear plate culvert,(4) presently, capable of connecting with city drainage system 1.5km across 108 National Road to planned sewage treatment plant, it is a 3×5m drainage canal.

龙湖街污水排水系统，全长3.7公里，排水断面2.5 X 3M的盖板涵。⑷穿越108国道至规划的污水处理厂目前可接入城市排水系统1.5公里，为3 X 5M的排水明渠。

The successful cloning of fulllength gG2 of HSV2 and a successful forecast to B cell epitope of gG2 provide evidences and references for developing diagnostic kits for herpes virus infection and finding better target sites of HSV2 vaccine.

成功构建单纯疱疹病毒2型（HSV2）gG2全长基因克隆，为单纯疱疹病毒感染的诊断试剂的研发及抗单纯疱疹病毒疫苗研究寻找更好的靶位点提供研究材料和参考。

The successful cloning of fulllength gG2 of HSV2 and a successful forecast to B cell epitope of gG2 provide evidences and references for developing diagnostic kits for herpes virus infection and finding target sites of HSV2 vaccine.

成功构建单纯疱疹病毒2型（HSV2）gG2全长基因克隆，为单纯疱疹病毒感染的诊断试剂的研发及抗单纯疱疹病毒疫苗寻找更好的靶位点提供研究材料和。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

Or that almost 60% of the total length of the 2,906 kilometre-long Brahmaputra River that floods India and Bangladesh every year is located in Tibet?

而每年在印度和孟加拉国发生水灾的全长2906公里的雅鲁藏布江大约有60%的部分绵延在青藏高原？

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。