全长的

The cloning of Actin lay a the theoretical foun- dation for the further expression and biological analysis of Actin full-length cDNA sequence.

土耳其斯坦东毕吸虫肌动蛋白的全长cDNA的克隆为进一步表达及其生物学性能的分析提供了理论基础。

Results The whole genome of CVB4 jlu06 strain consisted of 7 395 nucleotides encoding 2183 amino acids, which showed high homologies to other CVB4 strains, with the mutations of 10 amino acids. Phylogenetic analysis proved that CVB4 jlu06 strain showed close relationship to HumanCB4 and POLG_CXB4 strains while showed remote relationship to CXB2 strain. Unlike other non-diabetogenic strains, CVB4 jlu06 strain showed common amino acids with diabetogenic strains at 4 sites in 3A region of non-structural protein.

结果 CVB4 jlu06株基因组全长7395个核苷酸，编码2183个氨基酸，与其他CVB4株的同源性较高，且发生了10个氨基酸的变异，与HumanCB4和POLG_CXB4亲缘关系较近，与CXB2亲缘关系较远；CVB4 jlu06株与具有潜在致糖尿病性病毒株，在非结构蛋白3A区4个位点有共同的氨基酸，与非致病毒株不同。

Standard of ornamental design acceptance of work: Ornamental design project is finishing dry hind, just can check and accept; Check and accept ornamental design project, whether does the breed that should check ornamental design, norms, design accord with design requirement; Ornamental design should be installed firm, its quality asks to reach tolerance, should accord with: The level of form ornamental design and perpendicular tolerance, every meters must not be more than 5 millimeter, must not be more than 3 millimeter endlong; The tolerance of position of alone ornamental design, must not be more than 10 millimeter; Ying Guangjie of ornamental design surface, design clarity, juncture rigor, must not interstitial, warp, edge damage drops the blemish such as horn; Back up spends exterior Ying Guangjie, design clarity, without break.

花饰工程验收标准：花饰工程完工并干燥后，方可验收；验收花饰工程，应检查花饰的品种、规格、图案是否符合设计要求；花饰应安装牢固，其质量要求及允许偏差，应符合：条形花饰的水平和垂直允许偏差，每米不得大于5毫米，全长不得大于3毫米；单独花饰位置的允许偏差，不得大于10毫米；花饰表面应光洁，图案清晰，接缝严密，不得有裂缝、翘曲、缺棱掉角等缺陷；裱花表面应光洁，图案清晰、无裂缝。

The identification was made by means of restriction enzym eanalysis, PCR and DNA sequencing. Results: The murine entire coding cDNA of 2508bp was enamplified from spleen cells. There was 100% homogenous nucleotide sequence as compared with standard sequence (NM021297) of TLR4 from Genbank.

结果：扩增得到的小鼠TLR4基因cDNA全长2508bp，编码536个氨基酸，Blast结果分析表明，该cDNA与基因库中发表的序列(NM021297)具有100%的同源性。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

Capitata L., were dispersed along the chromosome lengths, with a predominant distribution in the pericentromeric or proximal regions and some heterochromatic arms.

大基因组的玉米和大麦的所有染色体都被密集地标记，并在染色体全长显示出强标记区与弱标记或不标记区的交替排列。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

A full length cDNA of 849bp with an entire ORF was obtained and its structure and function was predificated.

延伸了1个新基因片段的全长序列，获得501bp完整的开放阅读框架，表达166氨基酸，并验证其在多组织中的表达。

The genomic DNA were extracted from the leaves of Polygonatum roseum in Xinjiang and the primers were designed according to conservative sequences of Polygonatum lectins gene.

用百合科黄精属植物凝集素基因的保守序列为引物，从新疆黄精的叶中克隆出黄精凝集素的全长cDNA。

Coasting north rises to visit the big mouth stream outlet of have a common boundary with Heibei, the rubiginous needle stream outlet that visits have a common boundary with Jiangsu arrives south, full-length 3121 kilometers, occupy the 1/6 of countrywide coasting overall length about.

海岸线北起与河北省交界的大口河口，南到与江苏省交界的锈针河口，全长3121公里，约占全国海岸线总长的1/6。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。