全长的

Because the full length DFF45 recombinant protein was unstable in the solution, it means that either the DFF45 protein has uncompacted region in the structure or the protein has several domains.

由于细菌表达的全长DFF45重组蛋白表现出不稳定易降解的特点，说明该蛋白可能具有无固定结构的区域，或者含有多个结构域。

This work would lay the foundation for further study on regulating and expressing mechanism of MyoG in Gansu wapiti.

为此，本研究对甘肃马鹿MyoG基因的启动子区进行了克隆和生物信息学分析，以期为鹿MyoG基因全长序列的克隆、结构特征、表达调控机制及其突变与鹿产肉性能等方面的研究提供基础资料。

Witching Mechanism At 5' end of the RNA Transcript is a technology used in biology researching, So far, there is no review only about SMART technology.

MART (Switching Mechanism At 5' end of the RNA Transcript)技术是一项应用于生物科学研究领域的方法，能保证全长cDNA的获得，目前尚未见有关该技术的综述发表。

It is join Erie and an aqueduct that install rude lake, the river is winding and labyrinthian, the cloth way that state of American new York has south is much, north installs the Yang Ge of rude province to press down to Canada, full-length only 54 kilometers, altitude falls continuously from 174 meters however reach 75 meters, bizygomatic breadth of upriver reach river 3 kilometre of 2 ～, surface fall only 15 meters, current also relatively delay.

是连接伊利湖和安大略湖的一条水道，河流蜿蜒而曲折，南起美国纽约州的布法多，北至加拿大安大略省的杨格镇，全长仅54公里，海拔却从174米直降至75米，上游河段河面宽2～3千米，水面落差仅15米，水流也较缓。

AY506804 gene from apsella bursa-pastoris by rapid amplification of cDNA ends . The full-length cDNA of Capsella bursa-pastoris ICE1 gene (Cbice53) including complete 5'-UTR and 3'-UTR was 1811bp long, containing a 1476bp open reading frame which encodes a 492 amino acids protein.

本研究在荠菜还得到了一个新的ICE1基因（基因bank登录号：AY506804），该基因（Cbice53）全长cDNA为1811bp，含有一个1476bp的开放阅读框，预测编码一个492个氨基酸的蛋白。

A new CBF (Genbank accession no.: AY391121) gene was cloned from Gapsella bursa-pastoris by rapid amplification of cDNA ends . The full-length cDNA of Capsella bursa-pastoris CBF gene was 1034bp long and contained a 657bp open reading frame encoding a putative DRE/CRT -binding protein of 219 amino acids.

本文克隆得到了荠菜Cbcor15b转录激活因子Cbcbf（基因bank登录号：AY391121）全长cDNA为1034bp，含有一个657bp的开放阅读框，预测编码一个219个氨基酸的DRE/CRT的结合蛋白质。

Methods The Wistar rats were used as the experimental animal. On the back of each rat, a skin flap, measuring 1.5 cm×6 cm, caudally based, was elevated. Wound edges and the flap bed were separated from the flap itself by a silicone sheet. The VitC, bFGF, Magnesium sulfate and Papaverine were taken in the areas between flap and sheet. With 8 rats in each group, the drug-taken group was as trial group, while the physiological saline group was as control group. On the seventh day after the flap elevation, flap survival length was measured.

以鼠背部中线为中轴，以两侧髂嵴连线为蒂，形成一个1.5cm×6cm的单蒂皮瓣，其全长自皮肌层下与深筋膜游离，下垫置医用硅膜，硅膜以不露出切口，又起到阻隔真皮血运为宜，以小三角针用3-0丝线先缝合皮瓣两侧切口，然后将上述4种药物加入皮瓣与硅膜之间，再缝合皮瓣的头端将维生素C、bFGF、硫酸镁、罂粟碱分别作为实验组，以生理盐水作为空白对照组，每组8只动物，术后单笼喂养7d，取材并检测皮瓣存活的长度。

An infectious clone of the sat-RNA of BBSV-X was also constructed by placing the cDNA under the control of the 35S promoter. This plasmid could infect Chenopodium amaranticolor with the help of in vitro transcripts of BBSV-X.

构建了35S启动子控制下的BBSV卫星RNA全长侵染性克隆，侵染性检测表明，所获得的3个克隆均有较好的侵染性。

Methods: Five 10-23 deoxyribozymes (DZ1-DZ5) with 2 phosphorothioate groups at 5' and 3' end were designed and synthesized. Full sequence ET-1 RNA was transcripted and FAM-labeled 10-23 deoxyribozyme was used to select cleavable 10-23 deoxyribozyme in vitro and to detect intracellular uptake. The content of ET-1 mRNA was measured by semi-quantitative RT-PCR after 10-23 deoxyribozyme was transfected into cultured neonatal rat cardiomyocytes.

体外转录ET-1全长RNA底物，设计并合成5条ET-1 10-23脱氧核酶(DZ1~DZ5)，其5'及3'端各有2个核苷酸硫代修饰，体外切割ET-1RNA底物，筛选有效脱氧核酶；5'标记荧光素FAM的10-23脱氧核酶瞬间转染新生大鼠心肌细胞以观察对10-23脱氧核酶的摄取；采用半定量RT-PCR检测ET-1基因的表达。

Hutiao Corge Situated between the Haba Snow Mountains in the southwest of Shangri-la County and the Yulong Snow Mountains in the northwest of Lijiang, Hutiao Corge, 20 kilometers long and 213 meters high and with 18 dangerous shoals, is a gorge celebrated worldwide. It is divided into three sections: the upper Hutiao, the middle Hutiao and the lower Hutiao.

虎跳峡 是世界著名的大峡谷，位于香格里拉县东南部的哈巴雪山与丽江西北部的玉龙雪山之间，海拔落差3900多米，全长16公里，迂回线长约20公里，落差213米，分上虎跳、中虎跳、下虎跳三段，共18处险滩，江面最窄处仅30余米。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。