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Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后设计引物,经RT-PCR扩增、测序,获得了大白菜苹果酸脱氢酶基因的cDNA全长序列,并已将其登录到GenBank,登录号为:FJ208590,该cDNA全长1 209 bp,编码403个氨基酸。

On the D110, when mean total length reached about 86.5±5.9 mm, ovarian cavity grew bigger, and oocytes were firstly detected. Differentiation of testis was firstly observed in juvenile flounder with mean total length of 63.5±3.4 mm on the D90 when spermatogenous cell proliferated rapidly and spermaduct formed.

在90日龄、平均全长为63.5±3.4 mm的雄性牙鲆中,精原细胞快速增殖,并观察到了输精管结构;进一步的细胞学分化则出现在100日龄、平均全长为76.0±8.6 mm的个体中,此时可以看到精小叶的形成;在平均全长为140.0±15.2 mm时,精巢中出现初级精母细胞,标志着性腺分化的基本完成。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库;(2)经蓝白菌落筛选和斑点杂交,正向消减文库获得307个阳性克隆,反向消减文库获得78个阳性克隆;(3)正向消减文库挑选55个克隆成功测序,经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95%~100%),这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等;(4)反向消减文库挑选31个克隆成功测序,经同源性检索和比对最终确定差异表达基因片段16个,其中15个与人类全长基因具有很高相似性(95%~100%),包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性,表明它可能是未被克隆的人类基因EST片段。

However, the food composition of the M. albus with body length more than 100mm were quite consistent, with Chironomidae, Oligochaeta, Pheretima, Cladocera, Copepoda and insect larvae as the major food items.

而全长101-200mm的幼鳝及全长大于200mm的成鳝的食性,随全长变化相对稳定,且幼鳝和成鳝的食谱基本相同,其主要食物组成均包括摇蚊幼虫、水生寡毛、蚯蚓、昆虫幼虫、枝角类和桡足类等。

A gene sequence of the anthropogenic adiponectin spherical fragment obtained by the invention absolutely accords with a known gene sequence, and compared with the full-length adiponectin, the hydrolyzed anthropogenic adiponectin spherical fragment has stronger biological activity, fast reaction after dosage, good bioavailability and better clinic application prospect, especially when the diabetes patient receives a recanalization treatment after a myocardial infarction, the anemically myocardial damage of the diabetes patients can be eased by the exogenetic dosage.

本发明公开了一种人源性脂联素球状片段用于治疗糖尿病缺血性心脏病药物的应用,本发明所得到的脂联素球状片段的基因序列与已知的基因序列完全相符,与全长的脂联素相比,其水解以后的脂联素球状片段有着更强的生物学活性,而且给药后反应迅速,生物利用度好,有着更好的临床应用前景,特别是对糖尿病患者在发生心肌梗死后接受血管再通治疗时,通过外源性给药,以减轻糖尿病患者缺血心肌损伤。

Transcriptome comparison between indica and japonica (KOME full-length cDNAs) showed 2,693 NCGR unique cDNAs and 7,388 NCGR-KOME cDNA homologue pairs.

籼稻与粳稻的转录组(KOME全长cDNAs)比较分析表明,10,081条广陆矮4号cDNA中,7,388条与粳稻日本晴的全长cDNA有同源匹配,2,693条与粳稻日本晴的全长cDNA无同源匹配。

Through adding several the tuber of stemona full-length film is mixed thousands of collect is full-length TV program, the video below cereal song banner shared website YouTube to enter a brand-new period.

通过增添数百部全长电影和数千集全长电视节目,谷歌旗下视频分享网站YouTube进入了一个全新的时代。

The new developed anti-explosion structural model test devices are used and the integral anti-explosion reinforcement effect comparative simulation tests and study are made on cavities and free type anchor cables, full length grouted one and free type anchor cables as well as the full length type and the half length type anchor cables.

采用新研制的抗爆结构模型试验装置,对毛洞与自由式锚索,全长粘结式与自由式锚索以及全长粘结式锚索与半全长粘结式锚索对洞室的整体抗爆加固效应进行了对比模拟试验研究。

This paper studies the technology and properties of 60AT NbRE microalloy point rails heat treatment that rail head was heated by medium frequency induction and cooled by pressure air.

AT尖轨全长热处理采用中频感应加热、压缩空气吹冷淬火,对 6 0AT铌稀土微合金道岔尖轨进行轨头全长欠速淬火工艺及性能的研究。

Like Nike's adventure racing Air Zoom Orizaba, the Nike ACG Air Zoom Tallac has Zoom Air unit cushioning in the forefoot and an Air-Sole unit in the heel for full-length impact protection.

和nike的Air Zoom Orizaba一样,Nike Air Zoom Tallac Plus 同样整合了鞋头处的减震Zoom Air以及脚后跟处全长的防止冲撞的Air-Sole鞋底。

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