全长的

Specific primers was designed based on conserver sequence of the other animals,firstly,total DNA was extracted from blood of Mongolian sheep and the BMP15 DNA was amplified by PCR,The purified-PCR.product was cloned into PMD18-T vector.

根据其他物种BMP15基因的保守序列设计特异性引物，扩增蒙古羊的全长DNA序列。从蒙古羊的血液中提取总DNA，采用PCR技术扩增出蒙古羊BMP15DNA序列。

Methods 25 patients with upper tract transitional cell carcinoma underwent resection under Laparoscope and cystoscope. Integrity and the detent of ureterostoma was detected after the operation.

对25例肾盂肿瘤或中上段输尿管肿瘤的患者采取腹腔镜联合膀胱镜行肾、输尿管全长切除，术后检查手术标本的完整性，并行输尿管内压力测定，对输尿管口的封闭性进行评估。

The five sequences, encoding sIgM variable region, were likely to root in the four VH families. The expressions of sIgM, in spleen and head kidney of Xiphophorus helleri, were analyzed by semi-quantitative RT-PCR.

全长序列含有免疫球蛋白的信号序列：FKCLANH，得到的5个可变区序列可能来源于4个不同的VH家族。

Methods: According to the conserved regions of three genes reported, three pairs of specific primers were designed and used to amplify them from genomic DNA of soft hexaploid Chinese spring＇ s young leaves, Results: Through gene cloning and sequencing, they were 447bp, 447bp and 495bp in size, encoding 148, 148 and 164 amino acid residues respectively.

根据已报导的小麦3种基因的保守序列，设计合成了3对特异性引物，对六倍体软质小麦中国春基因组DNA进行基因扩增、克隆和序列测定，得到了3个基因的全长。

Strains containing cry1 genes were the most abundant in our collection (66%), and 21 different cry1-type gene combinations were found. Furthermore, several novel holotype cry genes were found and the full-length sequences of 3 novel cry genes were designated as cry54Aa1, cry30Fa1, and cry30Ga1 by B.

PCR-RFLP鉴定结果表明：此地区的苏云金芽胞杆菌主要含有cry1，cry2，cry3，cry4/10，cry9，cry30和 cry40 等7种cry基因类型；含cry1基因的菌株最丰富，共有21种不同cry1型基因组合；从中发现了新型模式基因，并采用Tail-PCR技术获得了其中3个基因的全长序列，被国际苏云金芽胞杆菌杀虫晶体蛋白基因命名委员会命名为cry54Aa1、cry30Fa1和cry30Ga1。

The growth hormone gene of Southern catfish is growth hormone cDNA full length sequence of Southern catfish obtained with hypophysis mRNA as template and through RT-PCR, 5'RACE and 3'RACE method.

本发明公开了南方鲇的生长激素基因，其特征是它以脑垂体的mRNA为模板经RT-PCR，5'RACE和3'RACE的方法得到南方鲇生长激素cDNA全长序列。

The cattle BMPRⅠA gene full-long cDNA is 4067bp, which encodes 532 amino acids and shares 91%, 93%, 89%, 88%, 91% and 82% similarity in nucleic acid sequences and 97%, 95%, 96%, 95%, 97% and 91% similarity in amino acid sequences with human, chimpanzee, mouse, rat, dog and red jungle fowl, respectively.

克隆得到了牛BMPRⅠA基因4067bp的cDNA全长序列，通过核酸序列分析发现，该基因编码532个氨基酸，与人、黑猩猩、小鼠、大鼠、犬和红原鸡在核酸序列上分别有91%，93%，89%，88%，91%和82%的同源性；在氨基酸序列上分别有97%，95%，96%，95%，97%和91%的同源性。

However, when backpacking, the low-cut boots do not offer as much stability and ankle protection as the full-length models.

但是，当背包，低切皮鞋不提供尽可能多的稳定和脚踝的全长模型的保护。

Methylation is negative correlation with genetic transcription , and the mechanism is probably as follows : directly interfere the combination of specific transcripton and recognition site of each promoter ; mediated transcription inhibition by change the construction of chromatin; induced gene silencing by combination of methylation site and transcription inhibition. P16 gene is also called multiple tumor supresser gene , which is the best easily to methylate in the anti-oncogenes. P16 gene locates in 9p21 of mankind-chromatosome , and it's total length is 8.5kb .

甲基化与基因转录呈负相关，其机制可能是[3]：直接干扰特异的转录子和各自启动子识别位点的结合；通过改变染色质结构，介导转录抑制；甲基化DNA位点直接与转录抑制子结合，诱导基因沉默。p16基因又称为多肿瘤抑制基因( multiple tumor suppressor 1 ，MTS1) [4] ，是肺癌中最易发生甲基化的抑癌基因，该基因位于人类染色体9p21上，全长8.5kb ，由两个内含子和三个外显子组成。

After every 60μl of 30% HRP were injected into abdominal section of the esophagus of 20 rats respectively,the labelled neurons were found bilaterally within nodose ganglions of vagus nerve,dorsal root ganglia(T9～L3),thoracic sympathetic ganglions and coeliac ganglion .

将30％辣根过氧化酶溶液注入大鼠食管腹段近贲门处外膜下，切片用四甲基联苯胺法成色，结果如下：标记细胞主要分布于双侧结状神经节，双侧的脊神经节（主要在T9～L3）、双侧的胸交感节（T9～L1）和腹腔节，在延髓的标记细胞见于疑核全长内，主要分布于疑核咀侧端。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。