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One cDNA clone was identified by immunoscreening from a cDNA expression library prepared from Babesia gibsoni merozoite mRNA and its complete nucleotide sequence was 2108bp in length,including one single open reading frame and encoding a 62ku protein which had high homology with that of apical membrane antigen-1(AMA-1) of other species.

通过免疫筛选法,从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆,此cDNA核苷酸全长2108bp,包含1个完整的开放阅读框,编码蛋白的分子质量约为62ku。

Andrew, Scotland, with the 18-hole 72 Par and 7128 yards fairway, which facilitated with high quality creeping bent grass and Scotland straight bunkers on the total area of 1489mu.

占地面积1489亩,球道全长7128码。充分利用自然景观和植物,借鉴苏格兰林克斯风格,设有大面积的原始沙地和茂密的荒草,高品质的本特草球道碧绿如洗,苏格兰式直壁沙坑尽显不凡,整个球场自然、古朴、宁静、耐人寻味。

The DN mutant of fulllength VP22 had the strongest inhibitory effect, reducing the HBsAg concentration by 81.94%.

其中VP22全长构成的DN突变体具有最强的抑制效应,可使上清HBsAg浓度下降81.94%。

CONCLUSION摘要: Our successfully constructed cDNA libraries are fulllength libraries with high efficiency and can be screened by probes and antibodies to find the associated genes of the renal papillary adenocarcinoma.

结论摘要:我们构建的人肾乳头状腺癌及癌旁组织cDNA文库为高效、全长cDNA文库,可以用做探针、抗体等免疫学筛选,进一步探寻和肾乳头状腺癌相关的基因。

The scheme ensures that the LLR value of each bit can be calculated and provides highly reliable LLR by appropriately choosing some discarded paths, retaining them and extending them to fulllength paths.

为此,提出一种改进的软检测方案,通过合适地选取某些删除路径,将这些路径保留并延伸为全长路径,可以确保对每比特都能计算其LLR,并能提供可靠性高的LLR值。

We have successfully attained the fulllength cDNA sequence of FRG4, bioinformatic analysis shows FRG4 gene has 99%homology with Homosapiens synapse associated protein 1 (SYAP1), consists of 9 exons and 8 introns and located in Xp22.2. None transmembrane domains but a BSD domain was discovered, and its encoding protein is probably a water-solubility protein.

结果表明cDNA文库基础上运用热启动PCR获得FRG4全长cDNA序列,生物信息学分析显示FRG4基因与人类的突触相关蛋白有99%同源性;定位在X染色体的短臂2区2带2亚带2次亚带;由9个外显子和8个内含子组成;无跨膜区段;有一结构域BSD;该基因编码蛋白可能为一水溶性蛋白。

It included the oculomotor nerve, the total length of the posterior communicating artery,the posterior cerebral artery segment P1,P2, and the upper basilar artery.

包括动眼神经起始部至入海绵窦后壁,后交通动脉全长,大脑后动脉的P1P2段,基底动脉的上部和分叉部。

In this study, 2 individuals carrying RHD positive, D antigen negative allele (with exon 1 and 10 of RHD) and their mRNA were investigated by using reverse transcriptase PCR and sequencing through one pair of specific primers for 5′- and 3′-non-coding region of RHD, taking a Rh-positive sample as control.

本文分别针对RHD基因mRNA的5′-和3′-非编码区设计一对特异性引物,通过逆转录PCR和cDNA测序,分析2例RHD基因阳性(拥有第1和第10外显子)、D抗原表型阴性个体的全长mRNA/cDNA序列,同时以1例正常Rh阳性个体作对照。

But it was smaller than the former. Its base only get 108 meter and height only have 67 meter which is only 1/10 of Khufu.

但与前者相比,它的规模明显为细,底部只有108米和全长67米,是为胡夫金字塔的一成。

In 1969, Louisiana, Ryan lake was Mr. 2 causeway completion, it put mandela Mandeville and mathevon would connect 38.42 kilometers across, Mr, Ryan lake, which just eight miles you will only see land, water in the lake bridge over the oversight.

1969年,美国路易斯安那州的庞恰特雷恩湖2号堤道竣工,它把曼德韦尔和梅泰里连接起来,全长38.42千米,横跨庞恰特雷恩湖,其中有8英里你会只见水不见陆地,桥在湖的正中央纵贯而过。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。