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The cDNA is 987 base pairs of length and encodes a protein of 177 amino acid residues.

该cDNA全长987bp编码一个177氨基酸的蛋白,比较NM23-H1蛋白,NM23-H1B在氨基端多了额外的25个氨基酸。

Such studies are highly dependent on the efficient in vitro GLUT expression and, thus far, there has been no report of a system for high expression of GLUTs in vitro.

本实验利用分子生物学技术,获得了人类GLUT家族GLUT1、GLUT2和GLUT3三个家族成员的全长基因,构建了pESP-GLUTs表达载体,实现了GST-GLUT1、GST-GLUT2、GST-GLUT3融合蛋白在粟酒裂殖酵母中的高效表达。

An identical nucleotide sequence in various passages was obtained by analysis using DNASTAR software, to which the homologies of nucleotides of E2 gene of passages 14, 16, 17, 18 and 23 were 100%, 100%, 99.8%, 99.8%and 99.6%, and those of amino acids were 100%, 100%, 100%, 99.3%and 99.2%, respectively.

结果 Matsuba株E2基因全长846bp,传至23代时仍有很高的同源性,第14、16、17、18和23代与一致序列的核苷酸同源性分别为100%、100%、99.8%、99.8%和99.6%,氨基酸同源性分别为100%、100%、100%、99.3%和99.2%。

Lots of offshore construction methods have been adopted, such as erecting of the upper pylon shafts and main girders with huge floating cranes, prefabricating of the caissons in docks and floating of the caissons to their destinations.

里翁-安蒂里翁大桥采用斜拉桥主桥和两侧引桥组成的方案,其中主桥采用五跨连续结构,跨径组成为(286+3×560+286)m,全长2252m,里翁-安蒂里翁大桥建成后将成为世界上最长的斜拉桥(见图1)。

In addition, the sections of the bridge had to be winched into position over expanses of quicksand-like mudflats.

耗资达118亿元人民币的这座大桥全长36公里,南起宁波,北至浙江嘉兴,它把上海和宁波之间的车程从4个小时缩短到2个半小时。

By Nothern blot, this gene band located between 1383-1908bp maker, length of which was same with different gene obtained by subtractive hybridization. The results showed that the obtained wnt6 gene was a cDNA fragment with full open reading frame, which could be useful for further studies.

提取样本总RNA,与地高辛标记的Wnt6探针进行杂Northernblot杂交,结果阳性杂交带位于1383-1908bp处,与差异片段大小一致,验证了该基因确为全长cDNA片段,具有完整的开放读框,可以用于进一步研究。

EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length of open reading frame and 3'UTR sequence were cloned by RACE technique.

方法]采用简并引物PCR克隆得到黑唇鼠兔LDH-C基因的EST序列,再根据EST序列采用RACE技术克隆出基因的开放阅读框全长,和3'UTR序列。

An analysis of the effect of tooth path and optimum clearance angle on tool life in the up milling and down milling processes is presented.

就铣削轨迹长度而论,在相同的切削情况下顺铣的轨迹较短,但其差量甚小,仅占全长百分之几。

Considering the theory of roadway support in soft rock, we proposed an active support mode based on study of rheology of weak adjacent rock, complex stress environment, and destabilization of original supports. Fully grouted bolt, extension grouted cable that can strengthen self-bearing capacity and entirety are used. Original support scheme and reinforcement scheme are simulated by means of FLAC(superscript 3D).

在分析硐室围岩岩性、软弱围岩流变、复杂应力环境及原支护结构失稳的基础上,结合软岩巷道围岩控制理论,提出了以全长锚固锚杆、锚索增强围岩整体性和自承载能力的主动支护,并以底板锚注加底拱返修加固。

Furthermore, the cDNA sequences of the human and mouse homologues were cloned and named as mNTKL-BP1 and hNTKL-BP1 independently.

我们进一步用RACE等方法克隆得到人和鼠的同源基因mNTKL-BP1和hNTKL-BP1的全长cDNA序列。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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