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Objective To establish an effective technique in reservation and function reconstruction of a leg, in which all the muscles except gastrocnemius necrosed induced by the delayed necrosis of all the intermuscular vessels, the foot was linked only by skin, fascia and tibia, Methods The leg was linked with foot only by the skin, fascia, caput mediale of gastrocnemius and tibia when all the muscles, intermuscular vessels and fibula were removed.

目的 探索迟发性小腿全长肌间血管全部坏死而导致除腓肠肌内侧头以外小腿肌肉全部坏死,仅以皮肤、筋膜、胫骨连足的情况下肢体保留和功能重建的方法。

The results of cDNA sequencing examined that the sBD-1 cDNA contain a open reading frame of 192 bases which encoded a 64 amino acid prepro-peptide and the prepro-peptide contained the β–defensin consensus sequence of six invariantly spaced cysteine residues.

我们采用 cDNA 末端快速扩增即 RACE 技术,主要包括锚式 PCR RACE 技术和反向嵌套 PCR RACE 技术分别扩增出蒙古绵羊 sBD-1 的 3ˊ末端和 5ˊ末端,经测序得到 sBD-1 的 cDNA 全长序列。

Of the 2,693 NCGR-cDNAs that have no homologies in KOME cDNAs, 1,751 matched to japonica genomic sequences, and were expressed in both varietyes, while their expression varies between two subspecies.

2,693条与粳稻日本晴的全长cDNA无同源匹配cDNA中,1,751条NCGR-cDNA与日本晴的基因组序列有同源匹配。

Results The full-length variant-specific surface antigen gene fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp,编码1条长为713 个氨基酸残基的多肽链,含有一个简单的开放阅读框。

Results The full-length variant-specific surface antigen gone fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp,编码1条长为713个氨基酸残基的多肽链,含有一个简单的开放阅读框。

The results showed that total length of the leptocephali dramatically decreased during the 1st to the 4th day of experiment in the Stage II.

结果,实验第1天体长开始收缩到实验第4天的体长收缩到最短(全长范围20.60 mm ~21.69 mm)为StageⅡ的发育阶段。

There were two nucleotide mutations between F238 and Lumbricus rubellus F-III-2, which caused two amino acids mutations.

结果表明,蚓激酶基因全长为738bp,编码含7个氨基酸残基的信号肽序列和238个氨基酸残基的成熟肽序列。

Objective To obtain the full-length cDNA sequences of CYP2E1, CYP2D5, ECHS1, which may be related with non-alcoholic fatty liver disease, from Microtus fortis.

目的 克隆CYP2E1、CYP2D5、ECHS1三个与非酒精性脂肪肝可能相关基因的cDNA全长基因,为进一步研究非酒精性脂肪肝的发病机制提供依据。

This ORF included 1101bp, encoding 367 amino acids, 92% similar to human and mouse.

结果分离的ORF全长1101bp,编码367个氨基酸,与人鼠氨基酸序列92%同源;利用生物信息学软件分析出此蛋白的理化特性并预测了其一级、二级和高级结构。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞,探讨不同基因对骨髓基质干细胞成骨活性的影响,我们首先利用RT-PCR方法获得了全长BM P-2,VEGF 165及bFGF基因,克隆入真核表达载体pcDNA 3.0,鉴定正确后,经脂质体介导转入分离培养的大鼠骨髓基质干细胞,G 418筛选出稳定表达株。

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