全长

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

为验证该设想，本研究建立了一步RT-PCR技术扩增体系，并利用该体系扩增和分析了国内几种蔬菜作物部分CMV分离物的2b基因序列同源性，选择特定分离物的全长2b基因，先利用PVX侵染性载体初步分析其正义和反义序列间的互作，再构建植物表达载体转化烟草和番茄，并研究转基因烟草抗CMV的表现，获得了抗CMV的转基因烟草植株。

Objective: To obtain a full length cDNA of novel deltarnethrin-resistance related gene from Culex pipiens pallens and identify differential expression levels between resistant and susceptible strains.

目的：获取淡色库蚊抗药性相关基因NYD-MLC2的cDNA全长序列并鉴定其在抗性品系和敏感品系中的表达差异。

Laparoscope combined with cystoscope for the treatment of upper tract transitional cell carcinoma is safe and effective.

对肾盂癌和中、上段输尿管癌采取腹腔镜联合膀胱镜行肾、输尿管全长切除可闭合输尿管末端，避免术中肿瘤种植，是一种安全有效的手术方式。

Methods 25 patients with upper tract transitional cell carcinoma underwent resection under Laparoscope and cystoscope. Integrity and the detent of ureterostoma was detected after the operation.

对25例肾盂肿瘤或中上段输尿管肿瘤的患者采取腹腔镜联合膀胱镜行肾、输尿管全长切除，术后检查手术标本的完整性，并行输尿管内压力测定，对输尿管口的封闭性进行评估。

By sequencing randomly-selected clones from the library and usingbioinformatics, 73 novel expressed sequence tags were obtained, in which therewere 24 full-length cDNAs, including cytolysin gene, neurotoxin genes, and greenfluorescence gene. Functional study on these novel genes is an ongoing task in ourlaboratory.

对文库克隆进行序列测定和生物信息学分析，已经获得了73个海葵新表达序列标签，有24个全长cDNA，其中包括海葵细胞毒素、海葵神经毒素、绿色荧光蛋白等具有开发应用价值的新基因。

The cDNA has 752bp and encodes a protein of 216 amino acids. Thenascent Src l contained a signal peptide of 19 amino acids, a propart of 19 amino acidsand a 178-amino-acid mature Src l. The characteristic amphiphilic α-helix of cytolysin islocated at the N-terminus of the mature Src l.

根据文库的EST序列设计引物，从玫瑰红绿海葵克隆到一全长cDNA序列，命名为Src I，利用生物信息学方法对其进行了分析，结果表明，该cDNA序列编码一个216个氨基酸的海葵细胞毒素前体蛋白，它包括19个氨基酸的信号肽、19个氨基酸的propart motif和178个氨基酸的成熟蛋白。

It is located in central Dalmatia, in ibenik-Knin county, just a few kilometers northeast of the city of ibenik.

卡尔卡河全长723公里，三分之二以上流经一条100至200米深的峡谷，然后汇入一些湖波。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果：聚合酶链反应扩增结果与目的片段位置一致，测序结果表明为肝再生增强因子mRNA，基因全长378 bp，在人、牛、小鼠、斑马鱼等物种中高度保守。

Alignment with amino acids sequence of TIR from Carassius auratus gibelio, Homo sapiens and Danio rerio indicated, Phe728、Leu738、Tyr756 and Arg736 of Carassius auratus gibelio played an important role both in the receptor signaling and intracellular localization.

TLR3全长序列起始21个氨基酸残基为信号肽；50至695个氨基酸残基间包含15个富含亮氨酸的重复序列；704至726个氨基酸残基为跨膜区；754至897个氨基酸残基为与白介素-1受体高度同源的区域。

For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。